The protein import apparatus of chloroplasts

Routing of cytosolically synthesized precursor proteins into chloroplasts is a specific process which involves a multitude of soluble and membrane components. In this review we wil1 focus on early events of the translocation pathway of nuclear coded plastidic precursor proteins and compare import routes for polypeptide of the outer chloroplast envelope to that of internal chloroplast compartments. A number of proteins housed in the chloroplast envelopes have been implied to be involved in the translocation process, but so far a certain function has not been assigned to any of these proteins. The only exception could be an envelope localized hsc 70 homologue which could retain the import competence of a precursor protein in transit into the organelle

Relations between PTSD symptoms and pain interference among older adults

Both the older adult population and the frequency of natural disasters (e.g., hurricanes) increase in the U.S. each year. Consequently, understanding the health experiences that affect older adults specifically following a natural disaster is important for providing effective healthcare and promoting health expectancy. For example, the extent to which PTSD symptoms following a natural disaster contribute to pain severity (beyond the contributions of other psychosocial experiences such as depression) among older adults is not yet clear, and whether this contribution differs by gender or age has yet to be established. The aims of this study were (1) to determine whether symptoms of PTSD after a natural disaster uniquely contribute to pain interference in daily activities, and (2) to examine the potential moderating effects of gender and age on this relation. Secondary analyses were conducted using an existing longitudinal dataset from the Ongoing Research on Aging in New Jersey: Bettering Opportunities for Wellness in Life (ORANJ BOWL) project (N =1,809, MAge = 67.7 years, 68% women). Results showed that PTSD symptoms are a unique contributor to pain interference and that avoidance symptoms accounted for the greatest amount of variance among the PTSD symptoms subscales. However, neither age nor gender moderated the relation between PTSD (and avoidance) symptoms and pain interference. These results may assist public health officials with response planning to natural disasters, as resources and treatments can be provided to address the relation between PTSD and pain interference in the increasing population of older adults

Hope Mediates the Relation between Income and Subjective Well-Being

In this study, we examine whether the positive effect of income gains on subjective well-being (SWB) can in part be explained by the improved future prospects that are generated by a more solid financial situation. Using three-wave panel data from the US, we inspect the relation between changes in income, hope and SWB using a fixed-effects model. Results show that hope indeed partially mediates the relation between income and SWB, but only for monthly incomes over $1800. Positive expectations, on the other hand, mediate the relation for all income levels. From the two components of hope, agency, the belief that we can achieve our goals, seems to be the strongest mediator. We find no indications that extremely high levels of hope or expectations will harm SWB through disappointment

Adipose-derived stem/stromal cells in kidney transplantation: Status quo and future perspectives

Kidney transplantation (KT) is the gold standard treatment of end-stage renal disease. Despite progressive advances in organ preservation, surgical technique, intensive care, and immunosuppression, long-term allograft survival has not significantly improved. Among the many peri-operative complications that can jeopardize transplant outcomes, ischemia-reperfusion injury (IRI) deserves special consideration as it is associated with delayed graft function, acute rejection, and premature transplant loss. Over the years, several strategies have been proposed to mitigate the impact of IRI and favor tolerance, with rather disappointing results. There is mounting evidence that adipose stem/stromal cells (ASCs) possess specific characteristics that could help prevent, reduce, or reverse IRI. Immunomodulating and tolerogenic properties have also been suggested, thus leading to the development of ASC-based prophylactic and therapeutic strategies in pre-clinical and clinical models of renal IRI and allograft rejection. ASCs are copious, easy to harvest, and readily expandable in culture. Furthermore, ASCs can secrete extracellular vesicles (EV) which may act as powerful mediators of tissue repair and tolerance. In the present review, we discuss the current knowledge on the mechanisms of action and therapeutic opportunities offered by ASCs and ASC-derived EVs in the KT setting. Most relevant pre-clinical and clinical studies as well as actual limitations and future perspective are highlighted

Migraine and cluster headache show impaired neurosteroids patterns

Background: Perturbation of neuronal excitability contributes to migraine. Neurosteroids modulate the activity of γ-aminobutyric acid A and N-methyl-d-aspartate receptors, and might be involved in the pathogenesis of migraine. Here, we measured plasma levels of four neurosteroids, i.e., allopregnanolone, epiallopregnanolone, dehydroepiandrosterone and deydroepiandrosterone sulfate, in patients affected by episodic migraine, chronic migraine, or cluster headache. Methods: Nineteen female patients affected by episodic migraine, 51 female patients affected by chronic migraine, and 18 male patients affected by cluster headache were recruited to the study. Sex- and age-matched healthy control subjects (31 females and 16 males) were also recruited. Patients were clinically characterized by using validated questionnaires. Plasma neurosteroid levels were measured by liquid chromatography-tandem mass spectrometry. Results: We found disease-specific changes in neurosteroid levels in our study groups. For example, allopregnanolone levels were significantly increased in episodic migraine and chronic migraine patients than in control subjects, whereas they were reduced in patients affected by cluster headache. Dehydroepiandrosterone and dehydroepiandrosterone sulfate levels were reduced in patients affected by chronic migraine, but did not change in patients affected by cluster headache. Conclusion: We have shown for the first time that large and disease-specific changes in circulating neurosteroid levels are associated with chronic headache disorders, raising the interesting possibility that fluctuations of neurosteroids at their site of action might shape the natural course of migraine and cluster headache. Whether the observed changes in neurosteroids are genetically determined or rather result from exposure to environmental or intrinsic stressors is unknown. This might also be matter for further investigation because stress is a known triggering factor for headache attacks in both migraineurs and cluster headache patients

Hope, Goals, and Pathways: Further Validating the Hope Scale with Observer Ratings

Objective: For the past two decades, hope theory has been an important framework for conceptualizing goal pursuits. Surprisingly there has been little effort to test the underlying suppositions of hope theory or to further validate the Hope Scale. Method: In Study 1, participants (N = 162, Mage = 19, 61% female) completed the Hope Scale and nominated goals they would like to accomplish in the next few months. Goals were coded on several dimensions. In Study 2, participants (N = 118, Mage = 19, 59% female) completed the Hope Scale, measures of optimism and self-efficacy, and generated workable pathways for achieving standardized goals. Results: Hope scores predicted setting objectively important, prosocial, long-term, and challenging goals. Hope (but not optimism or self-efficacy) was associated with generating more pathways for standardized goals. Conclusions: The results of these studies generally support the tenets of hope theory and provide further validation for the Hope Scale. As expected, people with higher hope were more likely than their lower-hope counterparts to engage in what has been considered successful goal-setting behavior. Hope is associated with important goal-relevant behaviors and efforts to increase hopeful thought may be important in helping individuals to move toward important life outcomes

Processing graded feedback: Electrophysiological correlates of learning from small and large errors

Feedback processing is important for learning and therefore may affect the consolidation of skills. Considerable research demonstrates electrophysiological differences between correct and incorrect feedback, but how we learn from small versus large errors is usually overlooked. This study investigated electrophysiological differences when processing small or large error feedback during a time estimation task. Data from high-learners and low-learners were analyzed separately. In both high- and low-learners, large error feedback was associated with higher feedback-related negativity (FRN) and small error feedback was associated with a larger P300 and increased amplitude over the motor related areas of the left hemisphere. In addition, small error feedback induced larger desynchronization in the alpha and beta bands with distinctly different topographies between the two learning groups: The high-learners showed a more localized decrease in beta power over the left frontocentral areas, and the low-learners showed a widespread reduction in the alpha power following small error feedback. Furthermore, only the high-learners showed an increase in phase synchronization between the midfrontal and left central areas. Importantly, this synchronization was correlated to how well the participants consolidated the estimation of the time interval. Thus, although large errors were associated with higher FRN, small errors were associated with larger oscillatory responses, which was more evident in the high-learners. Altogether, our results suggest an important role of the motor areas in the processing of error feedback for skill consolidation

The effect of platelet rich plasma on the treatment of androgenetic alopecia compared to placebo

Objective: To assess the efficacy of plasma rich proteins (PRP) compared to placebo in the treatment of androgenetic alopecia (AGA). Design: Systematic literature review Methods: Searches were done in PubMed utilizing the terms PRP, plasma rich protein, autologous activated PRP, autologous activated plasma rich protein, androgenetic alopecia, placebo, hair count, hair density, hair follicles, hair loss, therapeutic effect, growth factors, dermatology, and regenerative medicine. Articles were excluded if they were published before 2013, involved animal studies, compared minoxidil only in their research, included only female participants, non-English articles, studies with no quantifiable data, review research articles, and injections besides the scalp Results: A meta-analysis yielded five adequate articles meeting both the inclusion and exclusion criteria including 143 patients. All five studies resulted in beneficial outcomes and statistically significant results in patients treated with PRP. Conclusion: PRP injections revealed clinically significant improvement in hair growth and density along with overall patient satisfaction. PRP injections were shown to be well tolerated with mild pain and without any major adverse effect

Pengaruh Pelatihan Berpikir Positif Terhadap Depresi Pada Penderita Diabetes Mellitus

Individu yang menderita diabetes mellitus mengalami banyak Perubahan dalam hidupnya mulai dari pengaturan pola makan, harus patuh kepada treatment pengobatan, kondisi tubuh yang menjadi mudah lemas, dan hal tersebut harus terjadi sepanjang hidupnya. Perubahan dalam hidup tersebut membuat penderita diabetes mellitus menunjukkan beberapa reaksi psikologis yang negatif seperti marah, merasa tidak berguna, kecemasan yang meningkat, stres dan berujung pada depresi. Tujuan penelitian ini adalah menurunkan depresi pada penderita diabetes mellitus dengan menggunakan pelatihan berpikir positif. Penelitian ini menggunakan metode dengan pendekatan Experiment Single Case Design. Subjek dalam penelitian ini adalah dua orang dewasa penderita diabetes mellitus yang mengalami depresi dan tidak sedang mengikuti pelatihan atau terapi psikologi lainnya. Pengumpulan data menggunakan BDI (Beck Depression Inventory). Analisis data menggunakan analisis kuantitatif dan kualitatif. Hasil penelitian menunjukkan bahwa pelatihan berpikir positif dapat menurunkan depresi pada penderita diabetes mellitus yang mengalami depresi