Chapter Coherent Resonant Properties of Cardiac Cells

Materials / States of matte

Photonics4All - The OmniLight Laboratory

<p>This video shows the presentation about the "OmniLight Laboratory" held by Dusan Chorvat (International Laser Center) at the European Optical Society (EOS) Annual Meeting (EOSAM) in Berlin 2016</p

Photonics for experiments - development of the "OmniLight Laboratory"

<p>The presentation describes the functions of the "OmniLght Laboratory". The “OmniLight Laboratory” creates a light-based scientific show to provide an interactive light laboratory for presentation and education of photonics. This edgy tool was developed by the Photonics4All Partner International Laser Centre as a prototype of modular instrumentation for light-based scientific shows, aiming to provide an interactive light laboratory for presentation and education of photonics in many related outreach activities (namely training workshops, exhibitions, scientific events).</p

Preparation of metal nanoparticles by femtosecond laser ablation

Nanoparticles (NPs) proved to have numerous applications in various fields, including biomedicine and environmental sciences. In this work, we designed and created an apparatus for fabrication of metal NPs directly in liquids initiated by femtosecond laser pulses. The laser parameters leading to ~10 μJ/pulse energy and 0.1 GW peak power resulted in predominantly spherical particles with the sizes varying from <10 nm to ~100 nm in diameter. NPs generated from Cobalt and Zinc targets were smaller in order of magnitude compared to that of Nickel. The fabricated NPs were characterized by scanning electron microscopy, while spectroscopic properties were investigated by absorption spectroscopy and spectrally resolved fluorescence imaging. We also tested the possible interaction of the created NPs with living algae for their potential use for environmental research. Employing such ultrashort laser opens route to provide on-demand production of NP's in-situ at even factory environment

Biosensing the presence of nanoparticles using endogenous fluorescence in live algae

&lt;p&gt;Nanoparticles (NPs) from various metals (Zinc, Nickel, Cobalt, Copper) were designed and fabricated by direct synthesis using femtosecond laser ablation in liquids. Employing confocal microscopy with spectral detection and fluorescence lifetime imaging (FLIM), we have evaluated interaction of fabricated NPs with living Chlorella sp. algae by means of their naturally presented endogenous fluorescence. Live cell imaging was done in spectral region 500-550 nm and 650-710 nm to evaluate the effect of NPs on both, the green and the red fluorescence that is derived from flavonoids/carotenoids and chlorophylls respectively. We observed fluorescence intensity decrease in the red spectral region by all but Ni NPs. The presence of NPs also lead to an increase in the blue fluorescence at 477-488 nm, possibly resulting from reflected light. Gathered observations constitute the first step towards creation of methodological approaches for fast natural biosensing of the effects of environmental pollution directly in live algae.&lt;/p&gt

Evaluation of newly synthesized potential NLO-phores for 2-photon and SHG imaging

&lt;p&gt;Our contribution is focused on broadening of the spectrum of available non-linear optical (NLO)-phores (contrast agents for nonlinear optical microscopy) by design and synthesis of new organic dyes with appropriate optical properties. One of the main pre-requisites of microscopy utilizing non-linear excitation is the existence of molecules that are able to provide NLO response for the second-harmonic generation (SHG) or for the two-photon excited fluorescence (TPEF). Many molecules naturally occurring in living tissue such as collagens or NAD(P)H were successfully used in this regard, but there is a natural interest in broadening of the spectrum of available NLO-phores. Gathered results confirm applicability of the newly synthesized dyes as new potential NLO-phores for confocal laser scanning microscopy with nonlinear excitation in rat aorta.&lt;/p&gt