Tentative identification of the apoproteins of iron-sulfur centers of Photosystem I

AbstractA newly purified Photosystem (PS) I particle is described, with still active iron-sulfur acceptors: A, B and X. Apart from the apoprotein of P700, 3 other main polypeptides of this particle are located at 20, 17 and 10 kDa, and two minor ones are detectable at 16.5 and 8 kDa. Both in vivo 35S labeling and carboxymethylation with iodo[14C]acetate show that most of the cysteine residues are located in the 8-kDa band. The amino acid composition of this band reveals important common features with small iron-sulfur proteins of the ferredoxin type

The health benefits of autonomous vehicles: public awareness and receptivity in Australia

Objectives: The substantial public health benefits of autonomous vehicles will be optimised once all vehicles operate in autonomous mode. This form of disruptive technology will need to be widely accepted by the community to facilitate the regulatory and behavioural adjustments required to achieve rapid adoption. The aim of this study was to assess: i) receptiveness to autonomous vehicles; ii) the salience of various health benefits (e.g. crash prevention, emission reduction, driving stress reduction, cyclist safety, increased mobility for those unable to drive); and iii) prompted awareness of these health benefits. Methods: Quantitative and qualitative data were generated via a national online survey of a broad range of Australians aged 16 years and over (n=1,624). Results: There were neutral levels of receptiveness and very low salience of health benefits, but more substantial levels of prompted awareness of positive health outcomes. Conclusions: These results suggest that the public may be interested in information relating to the individual and societal health benefits of autonomous vehicles, which in turn may increase overall support for this innovation. Implications for public health: Australians are likely to be receptive to autonomous vehicles when provided with information relating to their public health benefits

Heterogeneous Agglomeration

Many prior treatments of agglomeration explicitly or implicitly assume that all industries agglomerate for the same reasons. This paper uses U.K. establishment-level coagglomeration data to document substantial heterogeneity across industries in the microfoundations of agglomeration economies. It finds robust evidence of organizational and adaptive agglomeration forces as discussed by Chinitz (1961), Vernon (1960), and Jacobs (1969). These forces interact with the traditional Marshallian (1890) factors of input sharing, labor pooling, and knowledge spillovers, establishing a previously unrecognized complementarity between the approaches of Marshall and Jacobs, as well as others, to the analysis of agglomeration

Defining a city — delineating urban areas using cell-phone data

What is a city? Researchers use different criteria and datasets to define it—from population density to traffic flows. We argue there is one dataset that could serve as a proxy of the temporal and spatial connections that make cities what they are: geolocated data from the world’s more than 7 billion cell-phone users. Cell-phone data are a proxy of people’s presence in a given area and of their movement between areas. Combined with computational methods, these data can support city delineations that are dynamic, responding to multiple statistical and administrative requirements, and tailored to different research needs, thus accelerating ongoing work in urban science

Developing intermediate cities

Intermediate cities have experienced economic dynamism in recent years, but, with the focus firmly on large metropoles and sprawling megacities, the development potential of intermediate cities has stayed out of the limelight. This paper upholds the relevance and potential of intermediate cities, arguing that they can play as important a role – if not a more important one – than the large metropoles that, until now, have been the focus of attention. Intermediate cities hold considerable advantages, in particular for poverty reduction and as more efficient ecosystems to live and work. Untapping the potential of intermediate cities requires, however, more territorially balanced, place-sensitive strategies

Accelerated apoptotic death and <i>in vivo</i> turnover of erythrocytes in mice lacking functional mitogen- and stress-activated kinase MSK1/2

The mitogen- and stress-activated kinase MSK1/2 plays a decisive role in apoptosis. In analogy to apoptosis of nucleated cells, suicidal erythrocyte death called eryptosis is characterized by cell shrinkage and cell membrane scrambling leading to phosphatidylserine (PS) externalization. Here, we explored whether MSK1/2 participates in the regulation of eryptosis. To this end, erythrocytes were isolated from mice lacking functional MSK1/2 (msk−/−) and corresponding wild-type mice (msk+/+). Blood count, hematocrit, hemoglobin concentration and mean erythrocyte volume were similar in both msk−/− and msk+/+ mice, but reticulocyte count was significantly increased in msk−/− mice. Cell membrane PS exposure was similar in untreated msk−/− and msk+/+ erythrocytes, but was enhanced by pathophysiological cell stressors ex vivo such as hyperosmotic shock or energy depletion to significantly higher levels in msk−/− erythrocytes than in msk+/+ erythrocytes. Cell shrinkage following hyperosmotic shock and energy depletion, as well as hemolysis following decrease of extracellular osmolarity was more pronounced in msk−/− erythrocytes. The in vivo clearance of autologously-infused CFSE-labeled erythrocytes from circulating blood was faster in msk−/− mice. The spleens from msk−/− mice contained a significantly greater number of PS-exposing erythrocytes than spleens from msk+/+ mice. The present observations point to accelerated eryptosis and subsequent clearance of erythrocytes leading to enhanced erythrocyte turnover in MSK1/2-deficient mice