Collective bremsstrahlung emission from plasmas containing energetic particle fluxes

Collective bremsstrahlung emission from plasmas containing energetic particle fluxe

Acute neuroinflammation induces AIS structural plasticity in a NOX2-dependent manner

Background Chronic microglia-mediated inflammation and oxidative stress are well-characterized underlying factors in neurodegenerative disease, whereby reactive inflammatory microglia enhance ROS production and impact neuronal integrity. Recently, it has been shown that during chronic inflammation, neuronal integrity is compromised through targeted disruption of the axon initial segment (AIS), the axonal domain critical for action potential initiation. AIS disruption was associated with contact by reactive inflammatory microglia which wrap around the AIS, increasing association with disease progression. While it is clear that chronic microglial inflammation and enhanced ROS production impact neuronal integrity, little is known about how acute microglial inflammation influences AIS stability. Here, we demonstrate that acute neuroinflammation induces AIS structural plasticity in a ROS-mediated and calpain-dependent manner. Methods C57BL/6J and NOX2−/− mice were given a single injection of lipopolysaccharide (LPS; 5 mg/kg) or vehicle (0.9% saline, 10 mL/kg) and analyzed at 6 h–2 weeks post-injection. Anti-inflammatory Didox (250 mg/kg) or vehicle (0.9% saline, 10 mL/kg) was administered beginning 24 h post-LPS injection and continued for 5 days; animals were analyzed 1 week post-injection. Microglial inflammation was assessed using immunohistochemistry (IHC) and RT-qPCR, and AIS integrity was quantitatively analyzed using ankyrinG immunolabeling. Data were statistically compared by one-way or two-way ANOVA where mean differences were significant as assessed using Tukey’s post hoc analysis. Results LPS-induced neuroinflammation, characterized by enhanced microglial inflammation and increased expression of ROS-producing enzymes, altered AIS protein clustering. Importantly, inflammation-induced AIS changes were reversed following resolution of microglial inflammation. Modulation of the inflammatory response using anti-inflammatory Didox, even after significant AIS disruption occurred, increased the rate of AIS recovery. qPCR and IHC analysis revealed that expression of microglial NOX2, a ROS-producing enzyme, was significantly increased correlating with AIS disruption. Furthermore, ablation of NOX2 prevented inflammation-induced AIS plasticity, suggesting that ROS drive AIS structural plasticity. Conclusions In the presence of acute microglial inflammation, the AIS undergoes an adaptive change that is capable of spontaneous recovery. Moreover, recovery can be therapeutically accelerated. Together, these findings underscore the dynamic capabilities of this domain in the presence of a pathological insult and provide evidence that the AIS is a viable therapeutic target

Soft X-ray coronal spectra at low activity levels observed by RESIK

The quiet-Sun X-ray emission is important for deducing coronal heating mechanisms, but it has not been studied in detail since the Orbiting Solar Observatory (OSO) spacecraft era. Bragg crystal spectrometer X-ray observations have generally concentrated on flares and active regions. The high sensitivity of the RESIK (REntgenovsky Spectrometer s Izognutymi Kristalami) instrument on the CORONAS-F solar mission has enabled the X-ray emission from the quiet corona to be studied in a systematic way for the first time. Our aim is to deduce the physical conditions of the non-flaring corona from RESIK line intensities in several spectral ranges using both isothermal and multithermal assumptions. We selected and analyzed spectra in 312 quiet-Sun intervals in January and February 2003, sorting them into 5 groups according to activity level. For each group, the fluxes in selected spectral bands have been used to calculate values parameters for the best-fit that lead to a intensities characteristic of each group. We used both isothermal and multitemperature assumptions, the latter described by differential emission measure (DEM) distributions. RESIK spectra cover the wavelength range (3.3-6.1 A). This includes emission lines of highly ionized Si, S, Cl, Ar, and K, which are suitable for evaluating temperature and emission measure, were used. The RESIK spectra during these intervals of very low solar activity for the first time provide information on the temperature structure of the quiet corona. Although most of the emission seems to arise from plasma with a temperature between 2MK and 3MK, there is also evidence of a hotter plasma (T approx. 10MK) with an emission measure 3 orders smaller than the cooler component. Neither coronal nor photospheric element abundances appear to describe the observed spectra satisfactorily.Comment: Submitting 1 Latex and 7 figure file