9,652 research outputs found
Globular Clusters in the Sombrero Galaxy (NGC 4594)
The Sombrero galaxy, NGC 4594, contains the most numerous globular cluster
system of any nearby spiral. It is an ideal candidate in which to study the
globular clusters and contrast them with those in Local Group spirals. Here we
present B and I imaging from the CTIO Schmidt telescope which gives a
field-of-view of 31' x 31'. Using DAOPHOT we have detected over 400 globular
clusters and derived their magnitudes, B--I colors and photometric
metallicities. We have attempted to separate our sample into disk and
bulge/halo globular cluster populations, based on location in the galaxy. There
is some evidence that the disk population is more metal--rich than the
bulge/halo globular clusters, however contamination, dust reddening and small
number statistics makes this result very tentative. We find that the median
metallicity of the bulge/halo globular clusters is [Fe/H] = -0.8. This
metallicity is consistent with previous estimates based on smaller samples. It
is also similar to the metallicity predicted by the globular cluster
metallicity -- galaxy luminosity relation. As with our Galaxy, there is no
radial metallicity gradient in the halo globular clusters. This suggests that
the spheriodal component of NGC 4594 did not form by a dissipational process.Comment: 7 pages, Latex. To be published in the Astronomical Journal. Full
paper available at http://www.ucolick.org/~forbes/home.htm
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The effect of rule retrieval on activity in the default mode network.
The default mode network (DMN) is often associated with internally-directed cognition, distinct from the constraints of the external environment. However, a recent finding is that the DMN shows strong activation after large task switches during a demanding externally-directed task (Crittenden et al., 2015; Smith et al., 2018). Following other proposals, we have suggested that the DMN encodes cognitive or environmental context, and that context representations are momentarily strengthened during large cognitive switches, perhaps so that new activity can be checked against current environmental constraints. An alternative account, consistent with the role of the DMN in episodic memory, might be that switches to a substantially new task increase demands on rule retrieval. To test this alternative, we directly manipulated rule retrieval demands. Contrary to the retrieval account, increased retrieval demand led to reduced DMN activity, accompanied by increased activation in prefrontal and lateral parietal cognitive control areas. Unlike episodic retrieval, with its rich contextual representations, rule retrieval does not drive DMN activity. Accordingly, it cannot explain increased DMN activity during large cognitive switches
Activities of \gamma-ray emitting isotopes in rainwater from Greater Sudbury, Canada following the Fukushima incident
We report the activity measured in rainwater samples collected in the Greater
Sudbury area of eastern Canada on 3, 16, 20, and 26 April 2011. The samples
were gamma-ray counted in a germanium detector and the isotopes 131I and 137Cs,
produced by the fission of 235U, and 134Cs, produced by neutron capture on
133Cs, were observed at elevated levels compared to a reference sample of
ice-water. These elevated activities are ascribed to the accident at the
Fukushima Dai-ichi nuclear reactor complex in Japan that followed the 11 March
earthquake and tsunami. The activity levels observed at no time presented
health concerns.Comment: 4 pages, 8 figure
Cytochrome cM decreases photosynthesis under photomixotrophy in Synechocystis sp. PCC 6803
Photomixotrophy is a metabolic state that enables photosynthetic microorganisms to simultaneously perform photosynthesis and metabolism of imported organic carbon substrates. This process is complicated in cyanobacteria, since many, including Synechocystis sp. PCC 6803, conduct photosynthesis and respiration in an interlinked thylakoid membrane electron transport chain. Under photomixotrophy, the cell must therefore tightly regulate electron fluxes from photosynthetic and respiratory complexes. In this study, we demonstrate, via characterization of photosynthetic apparatus and the proteome, that photomixotrophic growth results in a gradual inhibition of QA- reoxidation in wild-type Synechocystis, which largely decreases photosynthesis over 3 d of growth. This process is circumvented by deleting the gene encoding cytochrome cM (CytM), a cryptic c-type heme protein widespread in cyanobacteria. The ΔCytM strain maintained active photosynthesis over the 3-d period, demonstrated by high photosynthetic O2 and CO2 fluxes and effective yields of PSI and PSII. Overall, this resulted in a higher growth rate compared to that of the wild type, which was maintained by accumulation of proteins involved in phosphate and metal uptake, and cofactor biosynthetic enzymes. While the exact role of CytM has not been determined, a mutant deficient in the thylakoid-localized respiratory terminal oxidases and CytM (ΔCox/Cyd/CytM) displayed a phenotype similar to that of ΔCytM under photomixotrophy. This, in combination with other physiological data, and in contrast to a previous hypothesis, suggests that CytM does not transfer electrons to these complexes. In summary, our data suggest that CytM may have a regulatory role in photomixotrophy by modulating the photosynthetic capacity of cells
One-pot access to L-5,6-dihalotryptophans and L-alknyltryptophans using tryptophan synthase
The authors thank the European Research Council under the European Union’s Seventh Framework Programme (FP7/2007-2013/ERC grant agreement no 614779, and the University of St Andrews for a studentship (to D. R. M. Smith).We report, for the first time, the use of tryptophan synthase in the generation of L- dihalotryptophans and L-alkynyltryptophans. These previously unpublished compounds will be useful tools in the generation of probes for chemical biology, in biosynthetic diversification and as convenient building blocks for synthesis.PostprintPeer reviewe
Common Rocks, Minerals and Fossils Found in Indiana
Indiana Geological Survey Circular 3Rocks, minerals, and fossils of Indiana are the foundation, directly or indirectly, of the economic well-being of the state. Utilization of rocks, minerals, and fossils has contributed to the development of civilization. Those persons who have the ability, initiative, and vigor to utilize the earth's mineral resources advantageously are most likely to progress and prosper.
One of the responsibilities of the Indiana Geological Survey is the investigation of mineral wealth within the state. Not only should the citizens of Indiana be informed about these resources, but also people in other states should know that Indiana is a mining, as well as an agricultural and a manufacturing, state.
Because of rather extensive exploitation of limestone, dolomite, clay) shale, sand, gravel, coal, water, and oil resources in Indiana, more people are becoming aware that the science of geology exists and that much can be gained by an understanding of it. The interest of Hoosiers) as well as other people, in Indiana's rocks, minerals, and fossils is revealed both by the many inquiries received by the Geological Survey for information concerning Indiana's mineral resources and by the specimen samples sent to the Survey for identification. This circular has been prepared, therefore, primarily to accompany a set of common rocks, minerals, and fossils found in Indiana. These specimens are described in some detail in nontechnical terms in order that students in secondary schools, amateur collectors, scout organizations, and other interested persons and groups can become familiar with them.
The authors can neither cover fully in this brief report all characteristics and variations of any given specimen nor describe in detail the infinite varieties of each rock, mineral, and fossil found in Indiana. For special information regarding Indiana geology, the reader should consult the more detailed reports of the Geological Survey and its predecessors. The staff of the Indiana Geological Survey hope that the study of specimen rocks, minerals, and fossils. will help in answering some of the questions about Indiana geology.Indiana Department of Conservatio
The effects of cationic surfactants on marine biofilm growth on hydrogels
A method for the quantification of biofilm formation on hydrogel protective coatings for optical sensors and cameras has been developed using fluorescein diacetate (FDA) hydrolysis. In conjunction with these measurements the release of the fouling resistant cationic surfactants benzalkonium chloride, tallowbenzyldimethylammonium chloride and dicocodimethylammonium chloride was measured, using high performance liquid chromatography (HPLC), to enable correlation to be made between release and biofilm resistance and thus determine the active lifetime of such coatings. Results indicate that the twin-chained material, dicocodimethylammonium chloride, produced superior biofouling resistance as, at the 12 week time point, little fouling was detected on this coating. The hydrogel containing the long-chained tallowbenzyldimethylammonium chloride (mainly C-16 and C-18 chains) was the next best fouling resistant material, withstanding biofilm formation for 9 weeks. This correlates with the fact that each of these materials had an extremely slow to zero release rate, due to their irreversible binding to the hydrogel over the experimental timescale. In comparison the shorter chained benzalkonium chloride (mainly C-12 and C-14). showed signs of biofilm formation at the 3 week time point
The influence of cycling intensity upon cognitive response during inferred practice and competition conditions
Pif1-family helicases cooperatively suppress widespread replication-fork arrest at tRNA genes
Saccharomyces cerevisiae encodes two distinct Pif1-family helicases – Pif1 and Rrm3 – which have been reported to play distinct roles in numerous nuclear processes. Here, we systematically characterize the roles of Pif1 helicases in replisome progression and lagging-strand synthesis in S. cerevisiae. We demonstrate that either Pif1 or Rrm3 redundantly stimulate strand-displacement by DNA polymerase δ during lagging-strand synthesis. By analyzing replisome mobility in pif1 and rrm3 mutants, we show that Rrm3, with a partially redundant contribution from Pif1, suppresses widespread terminal arrest of the replisome at tRNA genes. Although both head-on and codirectional collisions induce replication fork arrest at tRNA genes, head-on collisions arrest a higher proportion of replisomes. Consistent with this observation, we find that head-on collisions between tRNA transcription and replication are under-represented in the S. cerevisiae genome. We demonstrate that tRNA-mediated arrest is R-loop independent, and propose that replisome arrest and DNA damage are mechanistically separable
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