2,969 research outputs found
Quantifying the efficiency and biases of forest Saccharomyces sampling strategies
Saccharomyces yeasts are emerging as model organisms for ecology and evolution, and researchers need environmental Saccharomyces isolates to test ecological and evolutionary hypotheses. However, methods for isolating Saccharomyces from nature have not been standardized and isolation methods may influence the genotypes and phenotypes of studied strains. We compared the effectiveness and potential biases of an established enrichment culturing method against a newly developed direct plating method for isolating forest floor Saccharomyces spp. In a European forest, enrichment culturing was both less successful at isolating S. paradoxus per sample collected and less labor intensive per isolated S. paradoxus colony than direct isolation. The two methods sampled similar S. paradoxus diversity: the number of unique genotypes sampled (i.e., genotypic diversity) per S. paradoxus isolate and average growth rates of S. paradoxus isolates did not differ between the two methods, and growth rate variances (i.e., phenotypic diversity) only differed in one of three tested environments. However, enrichment culturing did detect rare S. cerevisiae in the forest habitat, and also found two S. paradoxus isolates with outlier phenotypes. Our results validate the historically common method of using enrichment culturing to isolate representative collections of environmental Saccharomyces. We recommend that researchers choose a Saccharomyces sampling method based on resources available for sampling and isolate screening. Researchers interested in discovering new Saccharomyces phenotypes or rare Saccharomyces species from natural environments may also have more success using enrichment culturing. We include step-by-step sampling protocols in the supplemental materials
Fluctuations and Correlations in Lattice Models for Predator-Prey Interaction
Including spatial structure and stochastic noise invalidates the classical
Lotka-Volterra picture of stable regular population cycles emerging in models
for predator-prey interactions. Growth-limiting terms for the prey induce a
continuous extinction threshold for the predator population whose critical
properties are in the directed percolation universality class. Here, we discuss
the robustness of this scenario by considering an ecologically inspired
stochastic lattice predator-prey model variant where the predation process
includes next-nearest-neighbor interactions. We find that the corresponding
stochastic model reproduces the above scenario in dimensions 1< d \leq 4, in
contrast with mean-field theory which predicts a first-order phase transition.
However, the mean-field features are recovered upon allowing for
nearest-neighbor particle exchange processes, provided these are sufficiently
fast.Comment: 5 pages, 4 figures, 2-column revtex4 format. Emphasis on the lattice
predator-prey model with next-nearest-neighbor interaction (Rapid
Communication in PRE
Two-photon-induced photoconductivity enhancement in semiconductor microcavities: a theoretical investigation
We describe a detailed theoretical investigation of two-photon absorption photoconductivity in semiconductor microcavities. We show that high enhancement (by a factor of >10, 000) of the nonlinear response can be obtained as a result of the microcavity effect. We discuss in detail the design and performance (dynamic range, speed) of such a device with the help of the example of an AlGaAs/GaAs microcavity operating at 900 nm. This device shows promise for low-intensity, fast autocorrelation and demultiplexing applications
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Remarkable dynamics of nanoparticles in the urban atmosphere
Nanoparticles emitted from road traffic are the largest source of respiratory exposure for the general public living in urban areas. It has been suggested that the adverse health effects of airborne particles may scale with the airborne particle number, which if correct, focuses attention on the nanoparticle (less than 100 nm) size range which dominates the number count in urban areas. Urban measurements of particle size distributions have tended to show a broadly similar pattern dominated by a mode centred on 20â30 nm diameter particles emitted by diesel engine exhaust. In this paper we report the results of measurements of particle number concentration and size distribution made in a major London park as well as on the BT Tower, 160 m high. These measurements taken during the REPARTEE project (Regents Park and BT Tower experiment) show a remarkable shift in particle size distributions with major losses of the smallest particle class as particles are advected away from the traffic source. In the Park, the traffic related mode at 20â30 nm diameter is much reduced with a new mode at <10 nm. Size distribution measurements also revealed higher number concentrations of sub-50 nm particles at the BT Tower during days affected by higher turbulence as determined by Doppler Lidar measurements and indicate a loss of nanoparticles from air aged during less turbulent conditions. These results suggest that nanoparticles are lost by evaporation, rather than coagulation processes. The results have major implications for understanding the impacts of traffic-generated particulate matter on human health
Validation of Dunbar's number in Twitter conversations
Modern society's increasing dependency on online tools for both work and
recreation opens up unique opportunities for the study of social interactions.
A large survey of online exchanges or conversations on Twitter, collected
across six months involving 1.7 million individuals is presented here. We test
the theoretical cognitive limit on the number of stable social relationships
known as Dunbar's number. We find that users can entertain a maximum of 100-200
stable relationships in support for Dunbar's prediction. The "economy of
attention" is limited in the online world by cognitive and biological
constraints as predicted by Dunbar's theory. Inspired by this empirical
evidence we propose a simple dynamical mechanism, based on finite priority
queuing and time resources, that reproduces the observed social behavior.Comment: 8 pages, 6 figure
Identification of signaling pathways in early mammary gland development by mouse genetics
The mammary gland develops as an appendage of the ectoderm. The prenatal stage of mammary development is hormone independent and is regulated by sequential and reciprocal signaling between the epithelium and the mesenchyme. A number of recent studies using human and mouse genetics, in particular targeted gene deletion and transgenic expression, have identified some of the signals that control specific steps in development. This process involves cell specification and proliferation, reciprocal tissue interactions and cell migration. Since some of these events are recapitulated during tumorigenesis, an understanding of these signaling pathways may contribute to the development of targeted therapies and novel drugs
Widespread Receptivity to Neuropeptide PDF throughout the Neuronal Circadian Clock Network of Drosophila Revealed by Real-Time Cyclic AMP Imaging
SummaryThe neuropeptide PDF is released by sixteen clock neurons in Drosophila and helps maintain circadian activity rhythms by coordinating a network of âŒ150 neuronal clocks. Whether PDF acts directly on elements of this neural network remains unknown. We address this question by adapting Epac1-camps, a genetically encoded cAMP FRET sensor, for use in the living brain. We find that a subset of the PDF-expressing neurons respond to PDF with long-lasting cAMP increases and confirm that such responses require the PDF receptor. In contrast, an unrelated Drosophila neuropeptide, DH31, stimulates large cAMP increases in all PDF-expressing clock neurons. Thus, the network of âŒ150 clock neurons displays widespread, though not uniform, PDF receptivity. This work introduces a sensitive means of measuring cAMP changes in a living brain with subcellular resolution. Specifically, it experimentally confirms the longstanding hypothesis that PDF is a direct modulator of most neurons in the Drosophila clock network
Seasonal effects on reconciliation in Macaca Fuscata Yakui
Dietary composition may have profound effects on the activity budgets, levelof food competition, and social behavior of a species. Similarly, in seasonally breeding species, the mating season is a period in which competition for mating partners increases, affecting amicable social interactions among group members. We analyzed the importance of the mating season and of seasonal variations in dietary composition and food competition on econciliation
in wild female Japanese macaques (Macaca fuscata yakui) on Yakushima Island, Japan. Yakushima macaques are appropriate subjects because they are seasonal breeders and their dietary composition significantly changes among the seasons. Though large differences occurred between the summer months and the winter and early spring months in activity budgets and the consumption of the main food sources, i.e., fruits, seeds, and leaves, the level
of food competition and conciliatory tendency remained unaffected. Conversely,conciliatory tendency is significantly lower during the mating season than in the nonmating season. Moreover, conciliatory tendency is lower when 1 or both female opponents is in estrous than when they are not. Thus the mating season has profound effects on reconciliation, whereas seasonal changes in activity budgets and dietary composition do not. The detrimental effects of the mating season on female social relationships and reconciliation may be due to the importance of female competition for access to male partners in multimale, multifemale societies
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