Cloning, expression and nuclear localization of human NPM3, a member of the nucleophosmin/nucleoplasmin family of nuclear chaperones

BACKGROUND: Studies suggest that the related proteins nucleoplasmin and nucleophosmin (also called B23, NO38 or numatrin) are nuclear chaperones that mediate the assembly of nucleosomes and ribosomes, respectively, and that these activities are accomplished through the binding of basic proteins via their acidic domains. Recently discovered and less well characterized members of this family of acidic phosphoproteins include mouse nucleophosmin/nucleoplasmin 3 (Npm3) and Xenopus NO29. Here we report the cloning and initial characterization of the human ortholog of Npm3. RESULTS: Human genomic and cDNA clones of NPM3 were isolated and sequenced. NPM3 lies 5.5 kb upstream of FGF8 and thus maps to chromosome 10q24-26. In addition to amino acid similarities, NPM3 shares many physical characteristics with the nucleophosmin/nucleoplasmin family, including an acidic domain, multiple potential phosphorylation sites and a putative nuclear localization signal. Comparative analyses of 14 members of this family from various metazoans suggest that Xenopus NO29 is a candidate ortholog of human and mouse NPM3, and they further group both proteins closer with the nucleoplasmins than with the nucleophosmins. Northern blot analysis revealed that NPM3 was strongly expressed in all 16 human tissues examined, with especially robust expression in pancreas and testis; lung displayed the lowest level of expression. An analysis of subcellular fractions of NIH3T3 cells expressing epitope-tagged NPM3 revealed that NPM3 protein was localized solely in the nucleus. CONCLUSIONS: Human NPM3 is an abundant and widely expressed protein with primarily nuclear localization. These biological activities, together with its physical relationship to the chaparones nucleoplasmin and nucleophosmin, are consistent with the proposed function of NPM3 as a molecular chaperone functioning in the nucleus

The LISA Pathfinder Mission

LISA Pathfinder (formerly known as SMART-2) is an European Space Agency mission designed to pave the way for the joint ESA/NASA Laser Interferometer Space Antenna (LISA) mission by testing in flight the critical technologies required for space-borne gravitational wave detection; it will put two test masses in a near-perfect gravitational free-fall and control and measure their motion with unprecedented accuracy. This is achieved through technology comprising inertial sensors, high precision laser metrology, drag-free control, and an ultra precise micro-Newton propulsion system. LISA Pathfinder (LPF) essentially mimics one arm of spaceborne gravitational wave detectors by shrinking the million kilometre scale armlengths down to a few tens of centimetres, giving up the sensitivity to gravitational waves, but keeping the measurement technology. The scientific objective of the LISA Pathfinder mission consists then of the first in-flight test of low frequency gravitational wave detection metrology. In this paper I will give a brief overview of the mission, focusing on scientific and technical goals

Yerel sektör politikalarının sistem dinamiği yaklaşımıyla analizi: Alanya turizm sektörü.

The purpose of this work is indicating System Dynamics Approach to be an appropriate tool for analysis of policies suggested for local sectors. Local sectors are very important for stability of the country’s economy as well as welfare of the local community. Therefore, feasible and effective policies should be created and implemented in order to contribute to the local sectoral development. But before any policy implementation, policy analysis is required on to evaluate whether effectiveness and feasibility would be ensured in alternative policies. In this study, firstly the issues of Local Sectoral Dynamics and Policies are examined and specifically, the situation in Alanya Tourism Sector is studied. Afterwards, in accordance with policy analysis steps, problems of Alanya Tourism Sector are detailed and alternative policies that would aid in solution of the problem are idenfined. For evaluation of alternative policies, Dynamics of Alanya Tourism Sector are modeled by System Dynamics approach and the ‘Formal Model’ is implemented in Stella 9.0.1. The model is simulated for all policy alternatives and the policy outcomes of each alternative are forecasted. Finally the performance of each policy are evaluated using the previously established criteria and combined policies having more superior outcomes than the present ones are created.M.S. - Master of Scienc

Gerhardi Dumbar, Reipublicae Daventriensi ab Actis, Analecta, Seu Vetera aliquot scripta inedita, ab ipso publici juris facta

Vorlageform des Erscheinungsvermerks: Apud Johannem Van WykErschienen: T. 1 (1719) - 3 (1722

Historical Biology

ix 500hal.;ill.;28 c

Lettre de Fred A. Dumbar à G. Baby

4 page

TOWARDS A CLASSIFICATION OF VISUAL IMPAIRMENT

For the rehabilitation of people with impaired vision, it is essential to have adequate (preferably quantitative) information about their residual visual functions. Special attention is given to the extra information provided by the results of measurement of the contrast sensitivity, especially in combination with the results of other measurements, such as the visual field, the amount of intraocular straylight and the visual acuity. The value of the contrast sensitivity function as a predictor of the extent of dysfunctioning in the visual activities of everyday life, such as outdoor vision, reading and recognition of faces, will be discussed. As far as this is concerned, a comparison is made between the value of visual acuity and contrast sensitivity as measures of the extent of vision and visual dysfunctioning

The Informational Patterns of Laughter

Abstract: Laughter is one of the most characteristic -and enigmatic- communicational traits of human individuals. Its analysis has to take into account a variety of emotional, social, cognitive, and communicational factors densely interconnected. In this article we study laughter just as an auditive signal (as a 'neutral' information carrier), and we compare its structure with the regular traits of linguistic signals. In the experimental records of human laughter that we have performed, the most noticeable trait is the disorder content of frequencies. In comparison with the sonograms of vowels, the information content of which appears as a characteristic, regular function of the first vibration modes of the dynamic system formed, for each vowel, by the vocal cords and the accompanying resonance of the vocalization apparatus, the sonograms of laughter are highly irregular. In the episodes of laughter, a highly random content in frequencies appears, reason why it cannot be considered as a genuine codification of patterned information like in linguistic signals. In order to numerically gauge the disorder content of laughter frequencies, we have performed several "entropy" measures of the spectra -trying to unambiguously identify spontaneous laughter from "faked", articulated laughter. Interestingly, Shannon's entropy (the most natural candidate) performs rather poorly