Nanofabricated biohybrid structures for controlled drug delivery

In medical science and health care, development of new approaches to\ud specifically treat a disease without damaging healthy tissue is of high importance. In this context, the use of drug delivery vehicles that can recognize a disease cell and can specifically and locally deliver a therapeutic agent to that cell offers a good perspective. In this thesis we present the design, fabrication, and characterization of a new hybrid vehicle for controlled drug delivery

Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution

Mechanosensitive (MS) ion channels are membrane proteins that detect and respond to membrane tension in all branches of life. In bacteria, MS channels prevent cells from lysing upon sudden hypoosmotic shock by opening and releasing solutes and water. Despite the importance of MS channels and ongoing efforts to explain their functioning, the molecular mechanism of MS channel gating remains elusive and controversial. Here we report a method that allows single-subunit resolution for manipulating and monitoring “mechanosensitive channel of large conductance” from Escherichia coli. We gradually changed the hydrophobicity of the pore constriction in this homopentameric protein by modifying a critical pore residue one subunit at a time. Our experimental results suggest that both channel opening and closing are initiated by the transmembrane 1 helix of a single subunit and that the participation of each of the five identical subunits in the structural transitions between the closed and open states is asymmetrical. Such a minimal change in the pore environment seems ideal for a fast and energy-efficient response to changes in the membrane tension.

Data for: Short Term Trading Model for Asian Equity Index Futures – Using Hurst Exponent

Historical daily price data series for Equity Index futures has been sourced from Investing.com and formatted in the appropriate format. Daily price data of last 10 years has been considered for this research for following Asian Equity Index futures: Japan’s Nikkei, South Korea’s KOSPI, Australia’s ASX, India’s Nifty, Hong Kong’s HSI & HSCEI and Taiwan’s TSEC. Raw data is uploaded in .csv format

Nanofabricated biomimetic structures for smart targeting and drug delivery

We present a new approach to hybrid artificial cells (AC) designed for specific targeting and active drug delivery by combining an impermeable non-biological scaffold with an artificial bilayer lipid membrane (BLM) that supports the functioning bio-molecules required to provide AC functionality. We report on the fabrication of the scaffold using nanotechnology, as well as on loading of the scaffold and the functionalization of the AC. The results presented here are a first step towards the development of a biomimetic AC using nanotechnology