Ecoliteracy through imagery: a close reading of two wordless picture books

The purpose of this paper is to propose some ways of reading two wordless picture storybooks which have recently been published, by Suzy Lee and Bernardo Carvalho respectively. The objective is to analyse the ways in which the visual discourse can create an effective narrative using a variety of pictorial strategies. These works were not idly chosen, however. Both are concerned with ecoliteracy, having narratives that tell a story about a specific relationship between humans and the sea. Depicting maritime scenarios, the two picture books promote a special and symbolic approach to the natural environment. The narratives help to configure the environment as more than merely an undifferentiated space inhabited by living beings, this being the most elementary concept of ‘‘environment’’; rather, it is seen as an ecological arena involving the interaction between the landscape and the living beings it harbours. As such, the books assume the role of promoting not only habits of proto-literacy, but of ecoliteracy too.CIEC – FCT research unit 31

Trial application of a technique for human error analysis (ATHEANA)

The new method for HRA, ATHEANA, has been developed based on a study of the operating history of serious accidents and an understanding of the reasons why people make errors. Previous publications associated with the project have dealt with the theoretical framework under which errors occur and the retrospective analysis of operational events. This is the first attempt to use ATHEANA in a prospective way, to select and evaluate human errors within the PSA context

FACT, the Bur Kinase Pathway, and the Histone Co-Repressor HirC Have Overlapping Nucleosome-Related Roles in Yeast Transcription Elongation

Gene transcription is constrained by the nucleosomal nature of chromosomal DNA. This nucleosomal barrier is modulated by FACT, a conserved histone-binding heterodimer. FACT mediates transcription-linked nucleosome disassembly and also nucleosome reassembly in the wake of the RNA polymerase II transcription complex, and in this way maintains the repression of ‘cryptic’ promoters found within some genes. Here we focus on a novel mutant version of the yeast FACT subunit Spt16 that supplies essential Spt16 activities but impairs transcription-linked nucleosome reassembly in dominant fashion. This Spt16 mutant protein also has genetic effects that are recessive, which we used to show that certain Spt16 activities collaborate with histone acetylation and the activities of a Bur-kinase/Spt4–Spt5/Paf1C pathway that facilitate transcription elongation. These collaborating activities were opposed by the actions of Rpd3S, a histone deacetylase that restores a repressive chromatin environment in a transcription-linked manner. Spt16 activity paralleling that of HirC, a co-repressor of histone gene expression, was also found to be opposed by Rpd3S. Our findings suggest that Spt16, the Bur/Spt4–Spt5/Paf1C pathway, and normal histone abundance and/or stoichiometry, in mutually cooperative fashion, facilitate nucleosome disassembly during transcription elongation. The recessive nature of these effects of the mutant Spt16 protein on transcription-linked nucleosome disassembly, contrasted to its dominant negative effect on transcription-linked nucleosome reassembly, indicate that mutant FACT harbouring the mutant Spt16 protein competes poorly with normal FACT at the stage of transcription-linked nucleosome disassembly, but effectively with normal FACT for transcription-linked nucleosome reassembly. This functional difference is consistent with the idea that FACT association with the transcription elongation complex depends on nucleosome disassembly, and that the same FACT molecule that associates with an elongation complex through nucleosome disassembly is retained for reassembly of the same nucleosome

A Solve-RD ClinVar-based reanalysis of 1522 index cases from ERN-ITHACA reveals common pitfalls and misinterpretations in exome sequencing

Purpose Within the Solve-RD project (https://solve-rd.eu/), the European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies aimed to investigate whether a reanalysis of exomes from unsolved cases based on ClinVar annotations could establish additional diagnoses. We present the results of the “ClinVar low-hanging fruit” reanalysis, reasons for the failure of previous analyses, and lessons learned. Methods Data from the first 3576 exomes (1522 probands and 2054 relatives) collected from European Reference Network for Intellectual disability, TeleHealth, Autism and Congenital Anomalies was reanalyzed by the Solve-RD consortium by evaluating for the presence of single-nucleotide variant, and small insertions and deletions already reported as (likely) pathogenic in ClinVar. Variants were filtered according to frequency, genotype, and mode of inheritance and reinterpreted. Results We identified causal variants in 59 cases (3.9%), 50 of them also raised by other approaches and 9 leading to new diagnoses, highlighting interpretation challenges: variants in genes not known to be involved in human disease at the time of the first analysis, misleading genotypes, or variants undetected by local pipelines (variants in off-target regions, low quality filters, low allelic balance, or high frequency). Conclusion The “ClinVar low-hanging fruit” analysis represents an effective, fast, and easy approach to recover causal variants from exome sequencing data, herewith contributing to the reduction of the diagnostic deadlock

Alien Registration- Drouin, Mary (Biddeford, York County)

https://digitalmaine.com/alien_docs/1601/thumbnail.jp

Alien Registration- Drouin, Mary (Biddeford, York County)

https://digitalmaine.com/alien_docs/1601/thumbnail.jp

Alien Registration- Drouin, Mary (Biddeford, York County)

https://digitalmaine.com/alien_docs/1601/thumbnail.jp

Refinement of Impedance Models for Single and Two Degree of Freedom Linings based on Grazing Flow Duct Predictions and Measurements

A previously developed predictive model for impedance of single-degree-of-freedom and two-degree-of-freedom acoustic linings is extended to examine the effect of non-linearity on the assessment of fidelity of the model. Non-linearity was originally included in the impedance model via dependence of face sheet and septum flow resistance on acoustic particle velocity. Benchmarking was done by comparison of the acoustic field predicted by a propagation code with the imbedded impedance model and measurements made in a grazing flow duct test facility. The non-linear behavior of the lining model in the propagation code depended on the acoustic spectrum of the source. In this investigation the propagation code is extended to make the non-linear behavior of the lining model dependent on the local acoustic spectrum seen by the lining, accounting for the possibly significant local variation of sound pressure level created by the lining. This introduces an additional level of non-linearity that is approached by an iterative application of the propagation model. The principal conclusion drawn is that the significance of local variation of SPL is dependent on the increase of grazing flow resistance with grazing flow Mach number that tends to mask the flow resistance related to perforate flow resistance. The present study also reexamines the face sheet impedance model with the goal to improve the consistency of the linear resistance component. The septum impedance model is repeated for completeness, as is the grazing flow resistance model

Observations on Impedance Eduction in a Grazing Flow Duct Facility

This paper focuses on details of impedance eduction based on grazing flow duct testing. In this indirect approach the impedance of a sample of acoustic material, imbedded in one wall of the duct, is deduced (educed) from measurements of the acoustic field in the duct and comparison with a theoretical model of the acoustic field in the duct. The theoretical model is adjusted with a systematic variation of impedance to search for and achieve the best match of measurement and prediction. For measurement 0 ≤M ≤0.4 accuracy the mean flow is limited to approximately, and to a maximum frequency dependent on the duct geometry and grazing flow Mach number. The goal of the eduction procedure reported here is to provide a research quality capability to determine the impedance of samples of uniform normally reacting acoustic liners designed for turbo-fan engine inlet and by-pass ducts. Additional goals include relatively low cost of instrumentation and rapid turnaround of testing and data post-processing. Instrumentation includes only two microphones, one fixed reference microphone and one traversing microphone. The measured acoustic field is defined by FFT processing at multiple dwell points of the traversing microphone on the duct wall opposite to the lining sample to produce an acoustic transfer function. A finite element propagation code is used for theoretical prediction of the acoustic transfer function. Specific details addressed here include a unique implementation of the finite element method for the convected wave equation tailored for the duct geometry and frequency range. The propagation model allows the duct termination plane to be represented by measured acoustic pressure or by a separately educed termination impedance. Comparisons are made of educed impedance spectra obtained with the two termination choices and it is found that the best quality impedance spectra are obtained with the termination impedance boundary condition. A supporting analysis is provided to define the range of frequency and duct grazing flow Mach number for which the principal limitation of plane wave propagation can be achieved consistent with the placement of source and termination microphones. A refinement of the eduction process is the introduction of a two level search with an initial iteration based on a fixed effective duct Mach number accounting for the duct flow profile and a final iteration that allows effective Mach number to vary to produce improved iteration error. Multiple examples of educed impedance spectra for single and two degree of freedom liners representative of current designs document the quality that can be achieved, as well as limitations

Impedance Models For Single And Two Degree Of Freedom Linings With An Improved Data Base And Local Non-linearity

Previously developed predictive models for impedance of single-degree-of-freedom and two-degree-of-freedom acoustic linings driven by a broad band acoustic source are reexamined. Two issues are addressed, the first being improvement of the conventional perforate face sheet impedance model. Data correlations based on flow bench measurements of steady flow pressure drop are reevaluated with emphasis on low flow velocity to improve the consistency of the prediction of linear resistance. In addition, for two-degree-of-freedom linings, face sheet mass reactance is modified to account for the presence of the septum. The second issue addresses the implication that for a non-linear lining, with impedance a function of the local sound pressure level, the installed performance of the lining depends on the local impedance, as opposed to impedance based on the source sound pressure level. This is investigated in the benchmarking of the impedance models by comparison of the acoustic transfer function predicted by a propagation code with the imbedded impedance model and transfer function measurements made in a grazing flow duct test facility. The propagation code is extended to make the non-linear behavior of the lining model dependent on the local acoustic spectrum, introducing an additional level of non-linearity and an iterative application of the propagation code. A principal conclusion is that with no grazing flow both the lining model and grazing flow duct transfer function measurements show a significant effect of local variation of the acoustic spectrum. With increasing grazing flow Mach number, this effect is reduced and effectively disappears at the highest Mach number. With increasing grazing flow Mach number the grazing flow contribution to face sheet resistance dominates and tends to mask the non-linear behavior of the component of resistance not related to grazing flow