99 research outputs found
Disulphide bond reduction of a therapeutic monoclonal antibody during cell culture manufacturing operations
Earliest Triassic microbialites in the South China Block and other areas; controls on their growth and distribution
Earliest Triassic microbialites (ETMs) and inorganic carbonate crystal fans formed after the end-Permian mass extinction (ca. 251.4 Ma) within the basal Triassic Hindeodus parvus conodont zone. ETMs are distinguished from rarer, and more regional, subsequent Triassic microbialites. Large differences in ETMs between northern and southern areas of the South China block suggest geographic provinces, and ETMs are most abundant throughout the equatorial Tethys Ocean with further geographic variation. ETMs occur in shallow-marine shelves in a superanoxic stratified ocean and form the only widespread Phanerozoic microbialites with structures similar to those of the Cambro-Ordovician, and briefly after the latest Ordovician, Late Silurian and Late Devonian extinctions. ETMs disappeared long before the mid-Triassic biotic recovery, but it is not clear why, if they are interpreted as disaster taxa. In general, ETM occurrence suggests that microbially mediated calcification occurred where upwelled carbonate-rich anoxic waters mixed with warm aerated surface waters, forming regional dysoxia, so that extreme carbonate supersaturation and dysoxic conditions were both required for their growth. Long-term oceanic and atmospheric changes may have contributed to a trigger for ETM formation. In equatorial western Pangea, the earliest microbialites are late Early Triassic, but it is possible that ETMs could exist in western Pangea, if well-preserved earliest Triassic facies are discovered in future work
Light-Dependant Biostabilisation of Sediments by Stromatolite Assemblages
For the first time we have investigated the natural ecosystem engineering capacity of stromatolitic microbial assemblages. Stromatolites are laminated sedimentary structures formed by microbial activity and are considered to have dominated the shallows of the Precambrian oceans. Their fossilised remains are the most ancient unambiguous record of early life on earth. Stromatolites can therefore be considered as the first recognisable ecosystems on the planet. However, while many discussions have taken place over their structure and form, we have very little information on their functional ecology and how such assemblages persisted despite strong eternal forcing from wind and waves. The capture and binding of sediment is clearly a critical feature for the formation and persistence of stromatolite assemblages. Here, we investigated the ecosystem engineering capacity of stromatolitic microbial assemblages with respect to their ability to stabilise sediment using material from one of the few remaining living stromatolite systems (Highborne Cay, Bahamas). It was shown that the most effective assemblages could produce a rapid (12–24 h) and significant increase in sediment stability that continued in a linear fashion over the period of the experimentation (228 h). Importantly, it was also found that light was required for the assemblages to produce this stabilisation effect and that removal of assemblage into darkness could lead to a partial reversal of the stabilisation. This was attributed to the breakdown of extracellular polymeric substances under anaerobic conditions. These data were supported by microelectrode profiling of oxygen and calcium. The structure of the assemblages as they formed was visualised by low-temperature scanning electron microscopy and confocal laser microscopy. These results have implications for the understanding of early stromatolite development and highlight the potential importance of the evolution of photosynthesis in the mat forming process. The evolution of photosynthesis may have provided an important advance for the niche construction activity of microbial systems and the formation and persistence of the stromatolites which came to dominate shallow coastal environments for 80% of the biotic history of the earth
‘Albania: €1’ or the story of ‘big policies, small outcomes’: how Albania constructs and engages its diaspora
Since the fall of the communist regime in the early 1990s, Albania has experienced one of the most significant emigrations in the world as a share of its population. By 2010 almost half of its resident population was estimated to be living abroad – primarily in neighbouring Greece and Italy, but also in the UK and North America. This chapter discusses the emergence and establishment of the Albanian diaspora, its temporal and geographical diversity, and not least its involvement with Albania itself. Albania’s policymaking and key institutions are considered, with a focus on matters of citizenship; voting rights; the debate on migration and development; and not least the complex ways in which kin-state minority policies – related to ethnic Albanians living in Kosovo, Montenegro, southern Serbia, Macedonia and Greece – are interwoven with Albania’s emigration policies
Immunohistochemical localization and mRNA expression of aquaporins in the macula utriculi of patients with Meniere’s disease and acoustic neuroma
Meniere’s disease is nearly invariably associated with endolymphatic hydrops (the net accumulation of water in the inner ear endolymphatic space). Vestibular maculae utriculi were acquired from patients undergoing surgery for Meniere’s disease and acoustic neuroma and from autopsy (subjects with normal hearing and balance). Quantitative immunostaining was conducted with antibodies against aquaporins (AQPs) 1, 4, and 6, Na+K+ATPase, Na+K+2Cl co-transporter (NKCC1), and α-syntrophin. mRNA was extracted from the surgically acquired utricles from subjects with Meniere’s disease and acoustic neuroma to conduct quantitative real-time reverse transcription with polymerase chain reaction for AQP1, AQP4, and AQP6. AQP1 immunoreactivity (−IR) was located in blood vessels and fibrocytes in the underlying stroma, without any apparent alteration in Meniere’s specimens when compared with acoustic neuroma and autopsy specimens. AQP4-IR localized to the epithelial basolateral supporting cells in Meniere’s disease, acoustic neuroma, and autopsy. In specimens from subjects with Meniere’s disease, AQP4-IR was significantly decreased compared with autopsy and acoustic neuroma specimens. AQP6-IR occurred in the sub-apical vestibular supporting cells in acoustic neuroma and autopsy samples. However, in Meniere’s disease specimens, AQP6-IR was significantly increased and diffusely redistributed throughout the supporting cell cytoplasm. Na+K+ATPase, NKCC1, and α-syntrophin were expressed within sensory epithelia and were unaltered in Meniere’s disease specimens. Expression of AQP1, AQP4, or AQP6 mRNA did not differ in vestibular endorgans from patients with Meniere’s disease. Changes in AQP4 (decreased) and AQP6 (increased) expression in Meniere’s disease specimens suggest that the supporting cell might be a cellular target
The nuclear receptor ERβ engages AGO2 in regulation of gene transcription, RNA splicing and RISC loading
Current understanding of hypospadias: relevance of animal models
Hypospadias is a congenital abnormality of the penile urethra with an incidence of approximately 1:200-1:300 male births, which has doubled over the past three decades. The aetiology of the overwhelming majority of hypospadias remains unknown but appears to be a combination of genetic susceptibility and prenatal exposure to endocrine disruptors. Reliable animal models of hypospadias are required for better understanding of the mechanisms of normal penile urethral formation and hence hypospadias. Mice and/or rats are generally used for experimental modelling of hypospadias, however these do not fully reflect the human condition. To use these models successfully, researchers must understand the similarities and differences between mouse, rat and human penile anatomy as well as the normal morphogenetic mechanisms of penile development in these species. Despite some important differences, numerous features of animal and human hypospadias are shared: the prevalence of distal penile malformations; disruption of the urethral meatus; disruption of urethra-associated erectile bodies; and a common mechanism of impaired epithelial fusion events. Rat and mouse models of hypospadias are crucial to our understanding of hypospadias to ultimately reduce its incidence through better preventive strategies
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Reflux dolomitization – A Holocene example beneath a coastal salina, West Caicos Island, Turks and Caicos Islands
Holocene reflux dolomitization occurred beneath the Company Point evaporitic coastal salina on West Caicos Island, less than 1 km in from the edge of Caicos Platform, in the southeastern Bahamas. Poorly ordered, calcian-rich protodolomites replaced coralgal grainstones shed into the salina as storm-washover deposits, and also cemented them. Finer-grained carbonate sands were replaced by fine-crystalline dolomites, but coarser-grained skeletal fragments, including Halimeda sp., dissolved out to create late Holocene, precompactional secondary moldic porosity. Precipitation of overlying evaporitic deposits, principally gypsum, generated magnesium-rich brines. Below these evaporites, the downward increase in the degree of replacement dolomitization, toward the impermeable calcrete that caps Pleistocene grainstones at the base of the Holocene sequence, strongly implies reflux dolomitization.
Company Point dolomitization less than 1 km in from the western edge of Caicos Platform demonstrated that this process is not limited to platform- or ramp-interior settings well removed from the open ocean. A semi-arid climate combined with depositional ridge topography and periodic storm surge and groundwater recharge, allowed this coastal salina to initiate and evolve. As these beach-ridge and evaporitic deposits continue to prograde, as they are doing on the eastern side of West Caicos Island, then one can expect associated evaporitic deposits to have the potential not only to serve as an updip sealing facies, but to generate Mg-rich fluids to create precompactional porous dolostones with sheet-like reservoir quality.
•Documents Holocene reflux dolomitization beneath a coastal salina on Caicos Platform in the southeastern Bahamas.•Secondary porosity in Holocene dolomites is comparable to that seen in many subsurface dolostone hydrocarbon reservoirs.•There is potential to develop evaporitic deposits and attendant dolomitization in close proximity to open-ocean settings.•Progradational, evaporitic beach-ridge complexes can develop broad, sheet-like dolostone reservoirs with time and subsidence
Impact of strong easterly trade winds on carbonate petroleum exploration - Relationships developed from Caicos Platform, southeastern Bahamas
Brisk, persistent easterly trade wind influences define Holocene patterns of carbonate sedimentation across the Caicos Platform (southeastern Bahamas). Resultant predictive sedimentary facies models based on trade wind influences are more widely applicable to the exploration for subsurface carbonate plays than are existing models based on the northern Bahamas facies patterns, which are characterized by gentle trade winds and strong platform-margin-related oceanic processes (swells and tidal currents). The Caicos Platform relationships may be more applicable because many ancient shallow carbonate depositional environments were within the trade wind belts and commonly within broad intracratonic seas that were little influenced by oceanic processes.
The grainstone-dominated Caicos Platform exhibits reservoir potential over much of its surface, in contrast to northern Bahamian platforms, where oceanic tidal currents or swells and gentle easterly trade winds confine higher energy environments with reservoir potential to platform margins. Strong easterly trade winds across Caicos Platform promote widespread Holocene platform-interior oolitic, skeletal and grapestone grainstone bodies on this platform. Orientations of ooid sand bodies vary depending on preexisting topography, water depth and bottom energy. Shallow subtidal ooid sand shoals orient parallel to these winds. Ooid sands developed along older shorelines orient parallel to the shorelines but prograde perpendicular into these winds. Deeper platform-interior oolitic sands exist as widespread, sheet-like deposits. These trade winds allow reefs and ooids to coexist in many settings, permit isolated linear reefs to flourish on certain leeward platform margins, and promote effective off-bank transport of carbonate sands that create onlapping grainstone wedges. These relationships are very applicable to the rock record.
Strong trade wind influences, such as seen on Caicos Platform, better explain the occurrence of Cretaceous reef and/or oolitic grainstone reservoirs developed well in from platform margins (Fairway Field in East Texas; Black Lake Field in Louisiana) than do existing northern Bahamian models. Depositional models based on conditions in the northern Bahamian models would predict low-energy facies in these platform-interior settings. A trade-wind-driven depositional model, characterized by strong persistent easterlies, also better explains the origin of the onlapping wedge of skeletal grainstones at Poza Rica oilfield in Mexico.
The Caicos Platform easterly trade-wind-driven depositional model should generally apply to any ancient shallow carbonate environment that developed 5–22° north or south of the paleoequator, whether or not a platform margin was near by. Future carbonate exploration or exploitation should always factor in not only geological age, but also physiographic, latitudinal and climatic setting at a global and local scale.
•We present new models of tropical carbonate sedimentation from Caicos Platform.•Strong easterly trade winds expand the distribution of shallow-marine ooids and reefs.•Such winds create grainstone-dominated platform interiors and produce deep-water onlapping grainstone wedges.•Strong easterly trade-wind-driven carbonate depositional models better explain carbonate hydrocarbon play distribution.•Easterly trade-wind-driven carbonate depositional models expand subsurface carbonate hydrocarbon play potential
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