Interactions of charged particles with double-layer graphene

Nowadays we are witnesses of a development of one promising branch of applied physics, called plasmonics. Especially interesting issue is plasmonics in quasi-two-dimensional (q2D) crystals deposited on various dielectric substrates. Here arise the main questions: how to increase the 2D plasmon propagation length and how to excite 2D plasmon most efficiently. We will focus on describing the interaction between electronic excitations in two graphene layers with phonons in aluminium oxide (Al2O3) slab on which the graphene layers are deposited. Special attention will be paid to explain the hybridization between 2D plasmons and surface (TO) phonons. Because of multiple intersections of 2D plasmons and TO phonons the 2D plasmon moves to lower energies which allows it to be excited by charged particles moving at subthreshold speeds, [1]. We will present the results for wake potential induced by a charged particle which moves parallel to the graphene/dielectric interface. It will be shown how various substrates modify the efficiency of particle/plasmon coupling. The electronic excitations in graphene are obtained by using plane waveab initio approach where we have addressed the problem of calculation of EELS spectra in the optical limit ( → 0) and how to avoid intersupercell Coulomb interaction [2].28th Summer School and International Symposium on the Physics of Ionized Gases - SPIG 2016, August 29 - September 2, 2016, Belgrad

Entomological and ecological index for risk of infection causing lyme disease in territory of Vojvodina, Serbia

In Europe, of all the vector transmitted diseases, the occurrence of lyme disease is the one most often registered, and the most significant vector Borrelia burgdorferi is the tick Ixodes ricinus. Both humans and animals contract lyme disease. The risk of the occurrence of lyme disease is in correlation with potential exposure to tick bites and depends on the density of the tick population in the endemic area, the percentage of ticks infected with the cause of lyme disease, the duration and the nature of the activity of the susceptible population in a certain area. The objective of these investigations was to determine the entomological and the ecological risk index, as well as to assess the risk of transmission of the cause of lyme disease in the territory of Vojvodina Province in the Republic of Serbia. Ticks were collected at 12 locations in the South Bačka District of Vojvodina. A total of 1400 ticks were identified up to the level of species. After establishing the infection of ticks with the cause of lyme disease, the entomological and the ecological index was determined for the given regions using microscopic examination in a dark field. Two species of ticks aere identified in this geographic region (Ixodes ricinus and Dermacentor marginatus). Examining I. ricinus, the prevalence of infection B. burgdorferi was established, ranging up to 33.1%. The ecological risk index indicates that there is a potential risk of humans and animals becoming infected at 8 localities. It was determined for 3 localities that there is a definite actual risk of the transferrence of causes of lyme disease

The first year (2011) Implementation of New Projects

The first year (2011) Implementation of Projects of Department of Ethnology and Anthropology Faculty of Philosophy University of Belgrade, financed by the Ministry of Culture, Information and Information Society R

Concept of urban village: the application of the concept as a foundation for new typology of urban villages

Whole 20th century was marked with many new movements (“-isms”) in urbanism and architecture. Some of them, such as modernism and post-modernism, were especially important and influential. But, current situation is a bit different; there is no prevalent movement or concept. Many actual theories and concepts are the “mixes” of previous movements, so they can be described as “hybrid” ones. One of these hybrid concepts is the concept of urban village. The “hybridity” of the concept is visible in its name, which looks confusing at glance. But, the meaning of the concept is clear; it should be understood as a construct of sustainable community based on mixture of advantages from urban and rural/suburban life. The definition and main principles of the concept of urban village have been quite general, which has led to its wide application. Consequently, there are a bulk of new or renewed neighbourhoods and communities named as “urban villages” all over the World today. They often have various or even opponent characteristics. Thus, this gap between theoretical fundaments and application “in situ” has made the whole idea doubtful and unstable. This research tries to clarify this gap by the way of possible typology of urban villages. This proposition will be checked through theoretical explanation and the analysis of two different cases of urban villages. The research should present the stability of proposed typology hereof. Finally, whole research will accent the complexity of the concept of urban village in global context

The illegal settlements in Belgrade vs. taming city growth: case study of Belgrade

The paper will explore the genesis and growth of illegal settlements in Serbian capital, with the aim to present the specificity of informal housing areas and to offer a possible approach for taming its further growth. Serbia is, like some other Balkan countries, facing the problems of incoherent urban and regional development, of tackling the urban growth and of deficit of integrated urban strategies. On the other hand, specific problems of Serbia are related to the possibilities of integration of special socio-economic groups, such as low income citizens. This pressure of these problems is most visible in Belgrade since it deals with the demographic pressure and growth for a long time. As a consequence of this pressure many previously agriculture areas at the fringes of the city have become illegal settlements. On one hand, the paper will discuss spatial distribution and overall impact of illegal housing settlements in Belgrade territory, related to the sustainable urban growth and sustainable urban land use. One the other hand, it will offer the methodological framework given as the choice of aspects that should be treated in the process of integration of illegal settlements, while regulatory framework will point out the issues related to the shaping of settlements as liveable places. Conclusion remarks will emphasize the benefits and constraints of the chosen path for the integration process

Ispitivanje oksidativnog stresa i krvne slike ovaca odgajanih u regiji koja je bila izložena delovanju municije sa osiromašenim urnijumom (DU)

The paper presents the results of several health status parameters of sheep bred in the area exposed to depleted uranium (DU) ammunition during NATO bombing of Serbia and Montenegro in 1999. The blood samples of sheep were collected randomly in the region of Bujanovac, in 2004. Complete blood count was performed according to standard laboratory procedures. Concentration of red blood cells malondialdehyde (RBC MDA) and activity of erythrocyte superoxid dismutase (SOD) were determined spectrophotometrically, while the functional activity of leukocytes was investigated by NBT reduction and adhesion test. The results of complete red blood cells count indicated normocytic hypochromic anaemia. The total number of leukocytes and the differential leukocytes count were within the physiological range. Although the leukocytes adhesiveness was not changed in DU exposed animals, their increased NBT reduction revealed stimulated leukocytes' oxidative burst. This finding, together with significantly increased RBC MDA concentrations, as well as the activity of RBC antioxidant SOD, point to the existence of oxidative stress. Although the results indicated that animals were under oxidative stress, still there are no conclusive data that it was due to the exposure of DU that entered the environment during military operations.U ovom radu su prikazani rezultati ispitivanja zdravstvenog stanja ovaca odgajanih u regionu koji je bio izložen delovanju municije sa osiromašenim uranijumom (DU) tokom bombardovanja Srbije i Crne Gore 1999. godine, od strane NATO pakta. Uzorci krvi su uzeti tokom 2004. godine od ovaca u regionu Bujanovca, metodom slučajnog izbora. Kompletan pregled krvi obavljen je standardnim laboratorijskim procedurama. Koncentracija malondialdehida u ertirocitima (RBC MDA) i aktivnost eritrocitne superoksid dismutaze (SOD) određene su spektrofotometrijski, dok je funkcionalna aktivnost leukocita ispitana NBT testom redukcije i adhezije. Rezultati kompletnog pregleda eritrocita su ukazali na postojanje normocitne hipohromne anemije. Ukupan broj leukocita i leukocitna formula su bili u fiziološkim okvirima. Kod životinja izloženih delovanju osiromašenog uranijuma nije bila izmenjena adhezivnost leukocita, ali je pojačana NBT redukcija ukazivala na to da je bio podstaknut njihov oksidativni prasak. Ovaj nalaz, uz značajan porast koncentracije RBC MDA i aktivnosti eritrocitnog antioksidativnog enzima SOD, ukazivali su na postojanje oksidativnog stresa. Rezultati dobijeni ovim ispitivanjima su upućivali na to da su životinje bile pod oksidativnim stresom, ali nemamo sigurne podatke da je on bio posledica izlaganja dejstvu osiromašenog uranijuma (DU) koji je dospeo u njihovu životnu sredinu tokom vojne akcije u tom regionu

Development of SARS-CoV-2 N-protein specific capture ELISA

Ta na dijagnoza ljudi sa sumnjom na infekciju SARS-CoV-2 je od suštinskog zna aja za suzbijanje globalnog širenja COVID-19. Prisustvo SARS-CoV-2 može se otkriti RT-PCRom (otkriva RNK virusa) ili detekcijom prisustva virusnih antigena u biološkim te nostima ELISA-om ili sli nom tehnikom koje koriste antitela razvijena u životinjama. Cilj studije je bio uspostavljanje kvantitativnog testa koji se zasniva na koriš enju poliklonskih seruma za rutinsko odre ivanje koncentracije SARS-CoV-2 nukleokapsidnog proteina merenjam apsorbancije u standardnoj mikrotitarskoj plo ici sa 96 bunara. Za potrebe razvoja testa proizveden je rekombinantni N-protein i koriš en za proizvodnju antiseruma u miševima i ze evima. Proizvedeni antiserumi su pre iš eni i odre en im je titar. Poliklonskiantiserumi visokog afiniteta specifi ni za N-protein koriš eni su za razvoj ELISA testa specifi nog za ovaj protein. Test se zasniva na koriš enju poliklonskih seruma miševa koji su adherirani na dno bunara mikrotitarske plo ice za hvatanje N-proteina iz uzorka. Razli ite koncentracije rekombinantnog N-proteina su koriš ene za standardnu krivu za kvantifikaciju proteina. N-protein vezan za antitela miševa je detektovan ze jim poliklonskim serumom i anti-ze jim antitelom povezanim sa enzimom koji obezbe uje spektrofotometrijsko merenje. Uspešno smo razvili prototip ELISA testa za kvantifikaciju N-proteina sa granicom detekcije u opsegu od ng/mL. Prose na vrednost LOD za prototip ELISA testa za detekciju N-proteina je 9,2 ng/mL, dok je prose na vrednost LOQ 10,2 ng/mL. Pokazali smo da su proizvedeni poliklonski antiserumi pogodni za detekciju N-proteina sa sli nim ili boljim afinitetom i specifi noš u od komercijalnih antitela. Štaviše, prototip ELISA testa se može koristiti sa zadovoljavaju om pouzdanoš u za kvantifikaciju N-proteina u uzorcima bogatim proteinima, poput ljudskih seruma.The accurate diagnosis of people with suspected infection with the SARS-CoV-2 is essential to curb the global spread of COVID-19. The presence of SARS-CoV-2 can be detected by RT-PCR (it detects RNA of the virus) or by the presence of viral antigens in biological fluids in ELISA or similar techniques using antibodies developed in animals. The aim of the study was the establishment of a quantitative polyclonal sera-based test for routine measurement of the concentration of SARS CoV-2 nucleocapsid protein using absorbance measurement in a standard 96-well microtiter plate. For the purposes of the test development, recombinant N protein was produced and used for the production of mice and rabbit antisera. Produced antisera were purified and titer was determined. High-affinity polyclonal N-protein specific antisera were used for N-protein specific ELISA test development. The test is based on mice polyclonal sera adhered to microtiter plate bottom for the capture of the N protein from the specimen. Various concentrations of the recombinant N-protein were used to generate a standard curve for protein quantification. The N-protein bound to the mice antibodies was detected with rabbit polyclonal sera and anti-rabbit antibody coupled to an enzyme that provides spectrophotometric measurement. We have successfully developed the prototype ELISA for the quantification of N-protein with the detection limit being in the range of ng/mL. The average LOD value for the prototype ELISA was determined to be 9.2 ng/mL, while the average LOQ value was 10.2 ng/mL. We have demonstrated that produced polyclonal antisera are suitable for the detection of N-protein with affinity and specificity similar to, or better than commercial antibodies. Furthermore, the prototype ELISA can be used with satisfactory confidence for quantification of the N-protein in protein-rich samples, similar to human sera.Poster: [https://cherry.chem.bg.ac.rs/handle/123456789/5362

Role of dogs in contamination of urban environment with causes of parasitic zoonoses

Dogs belong to the group of animals that were the first to be domesticated. They live in cohabitation with humans and share their environment much more intimately than any other animal specie. The close contact between strays and pets, on the one side, and the pollution of urban areas with the feces of these animals, on the other, close the chain of infection with parasites, which jeopardizes also human health in the final link of that chain. Dogs are carriers and the true hosts to large numbers of species of zoonotic parasites - Cryptosporidium parvum, Giardia lamblia, Echinoccocus granulosus, Dipyllidium caninum, Toxocara canis, Ancylostomidae spp. and others, whose eggs or other developmental forms they eliminate into the environment through feces. The increase in the number of cases of toxocarosis in humans (syndrome of visceral larvae migrans), ancylostomosis (cutanea larvae migrans), hydatidosis, toxoplasmosis, or cryptosporidiosis are the best indicators of these relations. In order to resolve this problem, it is necessary to conduct systematic investigations of their parasitic fauna with the maximum cooperation of the animal owners, compulsory health education of the population in the area of the diseases that are transferred from animals to humans, and, certainly, carrying out the dehelminthization of dogs

Image of sustainable places

Contemporary moment requires redefinition and attentive attitude towards the phenomenon of place, his image, duration and especially the topic of his sustainability. The fact that all the architectural and urban design practice topical theme of responsibility and sustainability requires that such ideas are put into research focus and questions the concept, design thinking and design, and the planing critically assessed, analyzed and evaluated in the new framework of a paradigm. Images of contemporary place are reflected in images of modern cities, their parts, urban landscapes so they request to review the viability and make her to give new meaning seen in the idea of duration, the identity of place, scale and form.With this work we try to show some of the potential of the concept of sustainability through its basic building elements such as location, nature, time, duration, experience, form, change, and how these elements participate in the context of a new ideology of sustainability. Integrated and sustainable architectural design, which involves the formulation of ideas, concepts, shapes, materials, details and style, consistent with the properties of space on which to build, natural resources, capacities and needs of life processes will reflect, in many ways, to incorporate new process of thinking architecture, places and of its images, as the main impression (duration) of sustainability

Use of collaborative research between SME and University-research institutes to build new infant formula products

Breast milk is the best food for infants during the first 6 months of life, because of the valuable nutrition composition and presence of biologically active ingredients and therefore is considered the gold standard in feeding a baby. Understanding the role of human milk on infant health is fundamental in realising opportunities in the design and production of next generation infant formula. Scientific institutions have significant research expertise in examination of nutrition effect of food, designing formulas and technologies which can be accessed by food industry including production of infant formulas