Modelling Chlamydia and HPV co-infection in patient-derived ectocervix organoids reveals distinct cellular reprogramming

Coinfections with pathogenic microbes continually confront cervical mucosa, yet their implications in pathogenesis remain unclear. Lack of in-vitro models recapitulating cervical epithelium has been a bottleneck to study coinfections. Using patient-derived ectocervical organoids, we systematically modeled individual and coinfection dynamics of Human papillomavirus (HPV)16 E6E7 and Chlamydia, associated with carcinogenesis. The ectocervical stem cells were genetically manipulated to introduce E6E7 oncogenes to mimic HPV16 integration. Organoids from these stem cells develop the characteristics of precancerous lesions while retaining the self-renewal capacity and organize into mature stratified epithelium similar to healthy organoids. HPV16 E6E7 interferes with Chlamydia development and induces persistence. Unique transcriptional and post-translational responses induced by Chlamydia and HPV lead to distinct reprogramming of host cell processes. Strikingly, Chlamydia impedes HPV-induced mechanisms that maintain cellular and genome integrity, including mismatch repair in the stem cells. Together, our study employing organoids demonstrates the hazard of multiple infections and the unique cellular microenvironment they create, potentially contributing to neoplastic progression

Физические и технические аспекты технологии энергосберегающей сушки древесины в камерах с естественной циркуляцией агента

Теоретические и экспериментальные исследования, проведенные авторами, позволили создать технологию сушки древесины, позволяющую снизить энергоемкость процесса на 40-45 % при некотором (15-17 %) снижении производительности сушильного оборудования по сравнению с камерами, работающими с принудительной циркуляцией сушильного агента. The theoretical and experimental studies carried out by the authors made it possible to create a wood drying technology that reduces the energy consumption of the process by 40-45% with a certain (15-17 %) loss in the productivity of drying equipment compared to chambers operating with forced circulation of the drying agent

Physical and technical aspects of the technology of energysaving drying of wood in chambers with natural circulation of the agent

The problems of energy saving are one of the most urgent problems of modern industry and timber processing is no exception in this. In all wood processing, wood drying stands out for its energy consumption. A possible direction for significantly reducing the energy intensity of wood drying is the use of modes based on the phenomenon of thermal and moisture conductivity in chambers with a natural circulation of the drying agent. The theoretical and experimental studies carried out by the authors made it possible to create a wood drying technology that reduces the energy consumption of the process by 40-45% with a certain (15-17 %) loss in the productivity of drying equipment compared to chambers operating with forced circulation of the drying agent. © 2021 Institute of Physics Publishing. All rights reserved