PVP2005-71322 INTRODUCTION OF THE ELEMENT INTERACTION TECHNIQUE FOR WELDING ANALYSIS AND SIMULATION

ABSTRACT The residual stresses generated due to welding in pressure components may have several harmful effects such as decrease in the resistance to cycling load and corrosive environments. The analysis of the welding process has been developed extensively in 2D and 3D. The element movement technique has been shown to be very effective in simulating the filler material deposition leading to a reduction in the analysis time. However, when attempted for wider fields of applications, it had some limitations, especially when moving the elements towards the base-plate. In this paper, the element interaction technique is introduced utilizing the concepts of both the element movement and element birth techniques. The new technique is verified versus the currently developed procedures. In this technique, the elements of the weld pool are held in place in contact with the elements of the base-plate and the interaction is made to be a function of time. This gave several flexibilities in modeling the welding process. Hence, the technique is then used to analyze simple fillet welding of a plate and circumferential butt-welding of a pipe. INTRODUCTION The residual stresses generated during welding vary with the change in the welding parameters depending on the procedure of the joining process. Many researches have been done to investigate the residual stress for specific problem. Due to the complexity of the different applications, several assumptions and approximations, such as reducing the model from 3D to 2D, are made to facilitate the analysis. These approximations help mainly in reducing modeling efforts and analysis time. The element birth technique has been used in simulating metal deposition in both 3D and 2D models. Brown and Song [1] have used the technique in an axisymmetric simulation of a fillet-weld of ringstiffened cylinder. Wilkening and Snow [2] have also solved an axisymmetric simulation of a two-pass butt-weld of a cylinder using the element birth technique. Also, for a plate, they assumed that the through thickness variation of the residual stress is insignificant, so they modeled a single-pass butt-weld of plate using 2D plane model of the plate. Bouchard et a

The non-octarepeat copper binding site of the prion protein is a key regulator of prion conversion

The conversion of the prion protein (PrP(C)) into prions plays a key role in transmissible spongiform encephalopathies. Despite the importance for pathogenesis, the mechanism of prion formation has escaped detailed characterization due to the insoluble nature of prions. PrP(C) interacts with copper through octarepeat and non-octarepeat binding sites. Copper coordination to the non-octarepeat region has garnered interest due to the possibility that this interaction may impact prion conversion. We used X-ray absorption spectroscopy to study copper coordination at pH 5.5 and 7.0 in human PrP(C) constructs, either wild-type (WT) or carrying pathological mutations. We show that mutations and pH cause modifications of copper coordination in the non-octarepeat region. In the WT at pH 5.5, copper is anchored to His96 and His111, while at pH 7 it is coordinated by His111. Pathological point mutations alter the copper coordination at acidic conditions where the metal is anchored to His111. By using in vitro approaches, cell-based and computational techniques, we propose a model whereby PrP(C) coordinating copper with one His in the non-octarepeat region converts to prions at acidic condition. Thus, the non-octarepeat region may act as the long-sought-after prion switch, critical for disease onset and propagation

Propagation of RML Prions in Mice Expressing PrP Devoid of GPI Anchor Leads to Formation of a Novel, Stable Prion Strain

PrPC, a host protein which in prion-infected animals is converted to PrPSc, is linked to the cell membrane by a GPI anchor. Mice expressing PrPC without GPI anchor (tgGPI- mice), are susceptible to prion infection but accumulate anchorless PrPSc extra-, rather than intracellularly. We investigated whether tgGPI− mice could faithfully propagate prion strains despite the deviant structure and location of anchorless PrPSc. We found that RML and ME7, but not 22L prions propagated in tgGPI− brain developed novel cell tropisms, as determined by the Cell Panel Assay (CPA). Surprisingly, the levels of proteinase K-resistant PrPSc (PrPres) in RML- or ME7-infected tgGPI− brain were 25–50 times higher than in wild-type brain. When returned to wild-type brain, ME7 prions recovered their original properties, however RML prions had given rise to a novel prion strain, designated SFL, which remained unchanged even after three passages in wild-type mice. Because both RML PrPSc and SFL PrPSc are stably propagated in wild-type mice we propose that the two conformations are separated by a high activation energy barrier which is abrogated in tgGPI− mice

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An overview of common programming security vulnerabilities and possible solutions Proefschrift ingediend met het oog op het behalen van de graad van Licentiaat i

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Rapid advances in speech recognition theory, as well as computing hardware, have led to the development of machines that can take human speech as input, decode the information content of the speech, and respond accordingly. Real-time performance of such systems is often dominated by the evaluation of likelihoods in the statistical modeling component of the system. Statistical models are typically implemented using Gaussian mixture distributions. The primary objective of this thesis was to develop an extension of the Bucket Box Intersection algorithm in which the dimension with the optimal number of splits can be selected when multiple minima are present. The effects of normalization of mixture weights and Gaussian clipping have also been investigated. We show that the Extended BBI algorithm (EBBI) reduces run-time by 21 % without introducing any approximation error. EBBI also produced a 12 % lower word error rate than Gaussian clipping for the same computational complexity. These approaches were evaluated on a wide variety of tasks including conversational speech. DEDICATIO