Experimental Entanglement Concentration and Universal Bell-state Synthesizer

We report a novel Bell-state synthesizer in which an interferometric entanglement concentration scheme is used. An initially mixed polarization state from type-II spontaneous parametric down-conversion becomes entangled after the interferometric entanglement concentrator. This Bell-state synthesizer is universal in the sense that the output polarization state is not affected by spectral filtering, crystal thickness, and, most importantly, the choice of pump source. It is also robust against environmental disturbance and a more general state, partially mixed$-$partially entangled state, can be readily generated as well.Comment: Minor update (Newer data

Identification and characterization of stimulator of interferon genes as a robust adjuvant target for early life immunization

Immunization is key to preventing infectious diseases, a leading cause of death early in life. However, due to age-specific immunity, vaccines often demonstrate reduced efficacy in newborns and young infants as compared to adults. Here, we combined in vitro and in vivo approaches to identify adjuvant candidates for early life immunization. We employed newborn and adult bone marrow-derived dendritic cells (BMDCs) to perform a screening of pattern recognition receptor agonists and found that the stimulator of interferon genes ligand 2'3'-cGAMP (hereafter cGAMP) induces a comparable expression of surface maturation markers in newborn and adult BMDCs. Then, we utilized the trivalent recombinant hemagglutinin (rHA) influenza vaccine, Flublok, as a model antigen to investigate the role of cGAMP in adult and early life immunization. cGAMP adjuvantation alone could increase rHA-specific antibody titers in adult but not newborn mice. Remarkably, as compared to alum or cGAMP alone, immunization with cGAMP formulated with alum (Alhydrogel) enhanced newborn rHA-specific IgG2a/c titers ~400-fold, an antibody subclass associated with the development of IFN\uce\ub3-driven type 1 immunity in vivo and endowed with higher effector functions, by 42 days of life. Highlighting the amenability for successful vaccine formulation and delivery, we next confirmed that cGAMP adsorbs onto alum in vitro. Accordingly, immunization early in life with (cGAMP+alum) promoted IFN\uce\ub3 production by CD4+T cells and increased the proportions and absolute numbers of CD4+CXCR5+PD-1+T follicular helper and germinal center (GC) GL-7+CD138+B cells, suggesting an enhancement of the GC reaction. Adjuvantation effects were apparently specific for IgG2a/c isotype switching without effect on antibody affinity maturation, as there was no effect on rHA-specific IgG avidity. Overall, our studies suggest that cGAMP when formulated with alum may represent an effective adjuvantation system to foster humoral and cellular aspects of type 1 immunity for early life immunization

Multiple host-specificity loci of the broad host-range Rhizobium sp. NGR234 selected using the widely compatible legume Vigna unguiculata

International audienc

Multiple host-specificity loci of the broad host-range Rhizobium sp. NGR234 selected using the widely compatible legume Vigna unguiculata

International audienc