No Way Out? The Question of Unilateral Withdrawals or Referrals to the ICC and Other Human Rights Courts

This Article addresses the consequent issue: What if a state, self-referring or referring the situation in another country, changes its mind and attempts to withdraw its ICC referral? What is the role and appropriate response of the ICC at that point? This issue becomes especially relevant and pressing as events in Uganda unfold and the possibility looms of an attempted withdrawal of Uganda\u27s referral. This Article examines the Rome Statute, the drafting history, and the expert commentaries, together with the statutory and case law of the other major human rights courts and bodies, and the Vienna Convention on the Law of Treaties ( Vienna Convention ), in an effort to provide a comprehensive analysis of whether a state party can lawfully withdraw a referral from the ICC. To set the stage, it first examines the self-referral phenomenon and explores the reasons why attempted withdrawal of self-referrals is likely to arise

Permethrin and Fenvalerate Hydrolysis in Pseudoplusia Includens (Walker) and Heliothis Virescens (F.).

Fenvalerate, and cis- and trans-permethrin were hydrolyzed by eggs, larvae, pupae, and adults of P. includens and H. virescens. Hydrolysis (per larva) increased logarithmically to a maximum in last instars for the three pyrethroids in both insect species. trans-Permethrin hydrolysis was maximal in midguts, fat bodies, and cuticles of last instar P. includens in the late feeding stage. Changes in rates of hydrolysis appeared to influence toxicity. The midgut of both P. includens and H. virescens was generally the most active tissue in hydrolyzing the three pyrethroids tested. trans-Permethrin was generally hydrolyzed more rapidly than cis-permethrin or fenvalerate. cis- and trans-Permethrin were apparently hydrolyzed by the same enzyme from P. includens midguts, which had a molecular weight of ca. 80,000, a pI of 4.6-4.8, and a Km of 60 (mu)M. The enzyme was susceptible to inhibition by organophosphates, carbamates, chelators, and sulfhydryl groups reagents, and was closer in properties to leucine aminopeptidase than to alpha-naphthyl acetate esterase. Fenvalerate was hydrolyzed by at least four enzymes, with molecular weights of ca. 60,000, and pIs ranging from 4.6 to 6.8. The best organophosphate, chelating, and trifluoropropanone sulfide inhibitors of trans-permethrin hydrolysis in P. includens midguts were relatively nonpolar. Some significant synergism of trans-permethrin to P. includens was seen for compounds in these groups. Field collected H. virescens and P. includens generally hydrolyzed cis- and trans-permethrin and fenvalerate more rapidly than a lab strain. Feeding the lab strain on cotton or tobacco vs. artificial diet generally caused higher rates of hydrolysis in H. virescens, as did feeding the lab strain of P. includens on a resistant variety of soybeans. A strain of H. virescens from California (Imperial Valley) was more tolerant to trans-permethrin (12.5 X), cis-permethrin (5.4 X), and fenvalerate (2.5 X) than a lab strain, but trans-permethrin was equally toxic to both strains when synergized with profenofos. The rates of cis- and trans-permethrin hydrolysis were generally 2-3 X greater in third and last instars of the Imperial Valley than in the lab strain. Isoelectric focusing indicated increased levels and forms of enzyme activity in the Imperial Valley vs. lab strain

Field damage of sorghum (\u3ci\u3eSorghum bicolor\u3c/i\u3e) with reduced lignin levels by naturally occurring insect pests and pathogens

Sorghum (Sorghum bicolor (L.) Moench) brown midrib (bmr) mutant lines have reduced levels of lignin, which is a potentially useful trait for bioenergy production, but the effects of this trait on insect and plant pathogen interactions are unknown under field conditions. Field-grown bmr6, bmr12, and wild-type (WT) plants were examined for insect and disease damage. In most cases, observed frequency, population, or leaf area damage caused by insects or pathogens on bmr6 or bmr12 plants were not greater than those observed on WT plants in the field or laboratory assays. European corn borers [Ostrinia nubilalis (Hübner)(Lepidoptera: Pyralidae)] often caused lower amounts of leaf damage to bmr6 leaves compared to bmr12 and sometimes WT leaves in the field study. Leaf damage by corn earworms [Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae)] and fall armyworms [Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)] in laboratory assays was often lower for bmr versus WT leaves. Incidence of disease lesions was significantly higher on bmr6 compared to WT plants for one of three samplings in 2011, but the opposite trend was observed overall in 2012 and no significant differences were noted in 2013. When corn earworms and fall armyworms were fed the excised pith, bmr6 and/or bmr12 pith caused significant morality to one or both insect species in all 3 years. Damage variability between the 3 years may have been due to hotter and drier than normal conditions in 2012. Thus, bmr lines of sorghum suitable for bioenergy production have potential for sustainable production in the field

Issues in ATM Support of High-Performance, Geographically Distributed Computing

This report experimentally assesses the effect of the underlying network in a cluster-based computing environment. The assessment is quantified by application-level benchmarking, process-level communication, and network file input/output. Two testbeds were considered, one small cluster of Sun workstations and another large cluster composed of 32 high-end IBM RS/6000 platforms. The clusters had Ethernet, fiber distributed data interface (FDDI), Fibre Channel, and asynchronous transfer mode (ATM) network interface cards installed, providing the same processors and operating system for the entire suite of experiments. The primary goal of this report is to assess the suitability of an ATM-based, local-area network to support interprocess communication and remote file input/output systems for distributed computing

Survey of bacterial diversity in chronic wounds using Pyrosequencing, DGGE, and full ribosome shotgun sequencing

<p>Abstract</p> <p>Background</p> <p>Chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. These bacterial populations cooperate to promote their own survival and the chronic nature of the infection. Few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. The use of 3 separate16S-based molecular amplifications followed by pyrosequencing, shotgun Sanger sequencing, and denaturing gradient gel electrophoresis were utilized to survey the major populations of bacteria that occur in the pathogenic biofilms of three types of chronic wound types: diabetic foot ulcers (D), venous leg ulcers (V), and pressure ulcers (P).</p> <p>Results</p> <p>There are specific major populations of bacteria that were evident in the biofilms of all chronic wound types, including <it>Staphylococcus, Pseudomonas, Peptoniphilus, Enterobacter, Stenotrophomonas, Finegoldia</it>, and <it>Serratia </it>spp. Each of the wound types reveals marked differences in bacterial populations, such as pressure ulcers in which 62% of the populations were identified as obligate anaerobes. There were also populations of bacteria that were identified but not recognized as wound pathogens, such as <it>Abiotrophia para-adiacens </it>and <it>Rhodopseudomonas </it>spp. Results of molecular analyses were also compared to those obtained using traditional culture-based diagnostics. Only in one wound type did culture methods correctly identify the primary bacterial population indicating the need for improved diagnostic methods.</p> <p>Conclusion</p> <p>If clinicians can gain a better understanding of the wound's microbiota, it will give them a greater understanding of the wound's ecology and will allow them to better manage healing of the wound improving the prognosis of patients. This research highlights the necessity to begin evaluating, studying, and treating chronic wound pathogenic biofilms as multi-species entities in order to improve the outcomes of patients. This survey will also foster the pioneering and development of new molecular diagnostic tools, which can be used to identify the community compositions of chronic wound pathogenic biofilms and other medical biofilm infections.</p

Antimicrobial and Antiinsectan Phenolic Metabolites of Dalea searlsiae

Continued interest in the chemistry of Dalea spp. led to investigation of Dalea searlsiae, a plant native to areas of the western United States. Methanol extractions of D. searlsiae roots and subsequent chromatographic fractionation afforded the new prenylated and geranylated flavanones malheurans A–D (1–4) and known flavanones (5 and 6). Known rotenoids (7 and 8) and isoflavones (9 and 10) were isolated from aerial portions. Structure determination of pure compounds was accomplished primarily by extensive 1D- and 2D-NMR spectroscopy. The absolute configurations of compounds 1–5, 7, and 8 were assigned using electronic circular dichroism spectroscopy. Antimicrobial bioassays revealed significant activity concentrated in the plant roots. Compounds 1–6 exhibited MICs of 2–8 μg/mL against Streptococcus mutans, Bacillus cereus, and oxacillin-sensitive and -resistant Staphylococcus aureus. Aerial metabolites 7–10 were inactive against these organisms, but the presence of 7 and 8 prompted investigation of the antiinsectan activity of D. searlsiae metabolites toward the major crop pest Spodoptera frugiperda (fall armyworm). While compounds 1–10 all caused significant reductions in larval growth rates, associated mortality (33–66%) was highest with flavanone 4 and rotenoids 7 and 8. These findings suggest a differential allocation of antimicrobial and antiinsectan plant resources to root and aerial portions of the plant, respectively

Retirement and Socioeconomic Differences in Diurnal Cortisol: Longitudinal Evidence From a Cohort of British Civil Servants

Objectives: Early old age and the period around retirement are associated with a widening in socioeconomic inequalities in health. There are few studies that address the stress-biological factors related to this widening. This study examined whether retirement is associated with more advantageous (steeper) diurnal cortisol profiles, and differences in this association by occupational grade. Method: Data from the 7th (2002–2004), 8th (2006), and 9th (2007–09) phases of the London-based Whitehall II civil servants study were analysed. Thousand hundred and forty three respondents who were employed at phase 8 (mean age 59.9 years) and who had salivary cortisol measured from five samples collected across the day at phases 7 and 9 were analysed. Results: Retirement was associated with steeper diurnal slopes compared to those who remained in work. Employees in the lowest grades had flatter diurnal cortisol slopes compared to those in the highest grades. Low-grade retirees in particular had flatter diurnal slopes compared to high-grade retirees. Discussion: Socioeconomic differences in a biomarker associated with stress increase, rather than decrease, around the retirement period. These biological differences associated with transitions into retirement for different occupational groups may partly explain the pattern of widening social inequalities in health in early old age

Hepatitis C virus quasispecies and pseudotype analysis from acute infection to chronicity in HIV-1 co-infected individuals

HIV-1 infected patients who acquire HCV infection have higher rates of chronicity and liver disease progression than patients with HCV mono-infection. Understanding early events in this pathogenic process is important. We applied single genome sequencing of the E1 to NS3 regions and viral pseudotype neutralization assays to explore the consequences of viral quasispecies evolution from pre-seroconversion to chronicity in four co-infected individuals (mean follow up 566 days). We observed that one to three founder viruses were transmitted. Relatively low viral sequence diversity, possibly related to an impaired immune response, due to HIV infection was observed in three patients. However, the fourth patient, after an early purifying selection displayed increasing E2 sequence evolution, possibly related to being on suppressive antiretroviral therapy. Viral pseudotypes generated from HCV variants showed relative resistance to neutralization by autologous plasma but not to plasma collected from later time points, confirming ongoing virus escape from antibody neutralization