Providing Foundations for an Educational Revolution: Moving Towards an Integrated Perspective

The pandemic of Spring 2020 necessitated a rapid switch in teaching methods around the world. Most significantly was the revolutionary transition from face to face instruction to remote, distance, or virtual teaching/learning and the resultant online “new normal” that continues to ripple across the academy and society at large. This new reality has necessitated a paradigmatic shift in how scholars, teachers and administrators understand, create, employ, and assess teaching/learning. It has likewise resulted in a shift in how students, parents, families, and employers understand, value, desire, and prefer educational formats and settings. The authors point to the importance of considering aspects of theory, research, and best practices related to this transition. The article surveys resulting first response scholarship and forecast types of questions that loom large regarding the practice of online teaching in the new economic, academic, social framework

Eyewitnesses to the Suddenly Online Paradigm Shift in Education: Perspectives on the Experience, Sustaining Effective Teaching and Learning, and Forecasts for the Future

Introducing this special issue of the Journal of Literacy and Technology, the second part of the two-part special issues focusing on the COVID-19 “suddenly online” transition to remote/virtual eLearning modalities during the Spring of 2020. This article introduces the emergency voices from the field arising from the COVID-19 “suddenly online” transition to remote/virtual eLearning modalities during the Spring of 2020. This rare, and perhaps “once in a lifetime” momentous COVID-19 pandemic induced a paradigmatic shift in teaching and learning modalities. The first-hand eyewitness accounts which emerged from the turbulent months of the “suddenly online” transition in education are important to capture direct reports from participant observers of the experience. That in this case, many of these participant-observers are also trained educators, academic researchers, and able to provide meta-perspectives on those experiences makes recollections, reports, and perspectives even more remarkable and essential

Effect of Low Shear Modeled Microgravity (LSMMG) on the Probiotic Lactobacillus Acidophilus ATCC 4356

The introduction of generally recognized as safe (GRAS) probiotic microbes into the spaceflight food system has the potential for use as a safe, non-invasive, daily countermeasure to crew microbiome and immune dysregulation. However, the microgravity effects on the stress tolerances and genetic expression of probiotic bacteria must be determined to confirm translation of strain benefits and to identify potential for optimization of growth, survival, and strain selection for spaceflight. The work presented here demonstrates the translation of characteristics of a GRAS probiotic bacteria to a microgravity analog environment. Lactobacillus acidophilus ATCC 4356 was grown in the low shear modeled microgravity (LSMMG) orientation and the control orientation in the rotating wall vessel (RWV) to determine the effect of LSMMG on the growth, survival through stress challenge, and gene expression of the strain. No differences were observed between the LSMMG and control grown L. acidophilus, suggesting that the strain will behave similarly in spaceflight and may be expected to confer Earth-based benefits

Highly efficient 5\u27 capping of mitochondrial RNA with NAD+ and NADH by yeast and human mitochondrial RNA polymerase

Bacterial and eukaryotic nuclear RNA polymerases (RNAPs) cap RNA with the oxidized and reduced forms of the metabolic effector nicotinamide adenine dinucleotide, NAD+ and NADH, using NAD+ and NADH as non-canonical initiating nucleotides for transcription initiation. Here, we show that mitochondrial RNAPs (mtRNAPs) cap RNA with NAD+ and NADH, and do so more efficiently than nuclear RNAPs. Direct quantitation of NAD+- and NADH-capped RNA demonstrates remarkably high levels of capping in vivo: up to ~60% NAD+ and NADH capping of yeast mitochondrial transcripts, and up to ~15% NAD+ capping of human mitochondrial transcripts. The capping efficiency is determined by promoter sequence at, and upstream of, the transcription start site and, in yeast and human cells, by intracellular NAD+ and NADH levels. Our findings indicate mtRNAPs serve as both sensors and actuators in coupling cellular metabolism to mitochondrial transcriptional outputs, sensing NAD+ and NADH levels and adjusting transcriptional outputs accordingly. © 2018, Bird et al

An eddy-stimulated hotspot for fixed nitrogen-loss from the Peru oxygen minimum zone

Fixed nitrogen (N) loss to biogenic N2 in intense oceanic O2 minimum zones (OMZ) accounts for a large fraction of the global N sink and is an essential control on the ocean's N budget. However, major uncertainties exist regarding microbial pathways as well as net impact on the magnitude of N-loss and the ocean's overall N budget. Here we report the discovery of a N-loss hotspot in the Peru OMZ associated with a coastally trapped mesoscale eddy that is marked by an extreme N deficit matched by biogenic N2 production, high NO2− levels, and the highest isotope enrichments observed so far in OMZ's for the residual NO3−. High sea surface chlorophyll (SSC) in seaward flowing streamers provides evidence for offshore eddy transport of highly productive, inshore water. Resulting pulses in the downward flux of particles likely stimulated heterotrophic dissimilatory NO3− reduction and subsequent production of biogenic N2. The associated temporal/spatial heterogeneity of N-loss, mediated by a local succession of microbial processes, may explain inconsistencies observed among prior studies. Similar transient enhancements of N-loss likely occur within all other major OMZ's exerting a major influence on global ocean N and N isotope budgets

Chromosome looping at the human alpha-globin locus is mediated via the major upstream regulatory element (HS-40)

Previous studies in the mouse have shown that high levels of alpha-globin gene expression in late erythropoiesis depend on long-range, physical interactions between remote upstream regulatory elements and the globin promoters. Using quantitative chromosome conformation capture (q3C), we have now analyzed all interactions between 4 such elements lying 10 to 50 kb upstream of the human alpha cluster and their interactions with the alpha-globin promoter. All of these elements interact with the alpha-globin gene in an erythroid-specific manner. These results were confirmed in a mouse model of human alpha globin expression in which the human cluster replaces the mouse cluster in situ (humanized mouse). We have also shown that expression and all of the long-range interactions depend largely on just one of these elements; removal of the previously characterized major regulatory element (called HS -40) results in loss of all the interactions and alpha-globin expression. Reinsertion of this element at an ectopic location restores both expression and the intralocus interactions. In contrast to other more complex systems involving multiple upstream elements and promoters, analysis of the human alpha-globin cluster during erythropoiesis provides a simple and tractable model to understand the mechanisms underlying long-range gene regulation

Interim Report on Studies of the Mw ~7.9 Earthquake of 3 May 2006, Kingdom of Tonga

The very large and rare Mw ~7.9 Earthquake of 3 May 2006 in the Kingdom of Tonga aroused great interest among both Tongan scientists and their colleagues in Australia, New Zealand, and the United States. To investigate the earthquake we formed a collaborative research group of scientists from Australia, New Zealand, Tonga, and the United States. We brought in seven seismographs from Australia and the US to supplement the three-station network already in Tonga and eight GPS receivers primarily for the islands west of the earthquake epicenter. In addition, we made coastal observations to determine the regional pattern of subsidence associated with the earthquake. The GPS instruments can measure horizontal and vertical motion quite precisely, but only after the earthquake from the time of deployment onward, except for some sites on Tongatapu, Vava’u, and Lifuka that had been occupied by GPS receivers in the past. This report describes our efforts.Ministry of Lands and Survey, Kingdom of TongaGeoscience AustraliaGeological and Nuclear Sciences, New ZealandThe National Science Foundation, U.S.A.Institute for Geophysic