ESTIMATION OF SHEA TREES (Vitellaria paradoxa C.F. GAERTN.) FRUIT PRODUCTION BY ASSESSING THE CORRELATION BETWEEN YIELD PARAMETERS AND DENDROMETRIC FEATURES IN NORTHERN OF CÔTE D'IVOIRE

Vitellaria paradoxa, commonly known as the shea tree, is a tree of the family Sapotaceae and represents a traditional African food plant. It has been claimed to have the potential to improve nutrition, boost food supply, foster rural development, and support sustainable land care. Despite its multiple potentials, statistical data relating to its production are non-existent and/or unexploited in several African communities. To contrast this tendency, the present study aims to assess the intra-seasonal variation in fruit production of a sample of 115 shea trees and then to establish a correlation between yield parameters and several dendrometric features. Dendrometric (i.e. tree height, trunk girth, and crown basal area) and pomological (i.e. fruit and nut length and width) parameters, as well as yield parameters by monitoring daily fallen fruit from each sampled shea tree, carried out for five years consecutively, were considered for this study. The results showed inter-year fluctuation of shea fruit/nut number and shea fruit/nut weight. In addition, the results showed a significant increase in the annual average of shea fruit/nut yield per tree and as well per girth and/or crown basal area interval class, randomly generated by Sturge and Yule's formula. Interestingly, potentially high producing trees emerged within each considered interval class. Then, observed intraclass variation between trees determining shea yield can be exploited in selecting elite shea trees

Morphological diversity patterns among selected elite Shea trees (Vitellaria paradoxa C.F. Gaertn.) from Tchologo and Bagoué districts in Northern Côte d'Ivoire

peer reviewedAgromorphological diversity structure of the elite shea trees identified in village lands and conserved in situ in the districts of Bagoué and Tchologo by the shea breeding program of the University of Peleforo Gon Coulibaly (UPGC, Côte d’Ivoire), are not known. In the present study, we characterized the agromorphological parameters of 220 elite shea trees using a set of 12 quantitative traits. The results showed that elite shea trees population has been structured into three morphological clusters or genetic pools that do not overlap with the original geographic areas. Morphological Cluster I contain elite shea trees with small trunk diameters carrying large leaves and producing fewer fruits per tree. Morphological Cluster II consisted of elite shea trees with stronger trunks bearing small leaves and producing a high number of fruits per tree. Morphological Cluster III regrouped elite shea trees of medium trunk diameters carrying medium sized leaves; fruit production level is intermediate compare to preceding groups. The elite shea trees of morphological Clusters II, which are more interesting from an agronomic point of view, can be used as grafting trees for the production of high-yielding grafted plants for farmers in Côte d'Ivoire

Towards a re-emergence of chloroquine sensitivity in Côte d’Ivoire?

Abstract Background Resistance of Plasmodium falciparum to anti-malarial drugs has hampered efforts to eradicate malaria. Recent reports of a decline in the prevalence of chloroquine-resistant P. falciparum in several countries, including Malawi and Zambia, is raising the hope of reintroducing chloroquine in the near future, ideally in combination with another anti-malarial drug for the treatment of uncomplicated malaria. In Côte d’Ivoire, the decrease in the clinical efficacy of chloroquine, in addition to a high proportion of clinical isolates carrying the Thr-76 mutant allele of the pfcrt gene, had led to the discontinuation of the use of chloroquine in 2004. Previous studies have indicated the persistence of a high prevalence of the Thr-76 mutant allele despite the withdrawal of chloroquine as first-line anti-malarial drug. This present study is conducted to determine the prevalence of the Thr-76T mutant allele of the Pfcrt gene after a decade of the ban on the sale and use of chloroquine in Côte d’Ivoire. Results Analysis of the 64 sequences from all three study sites indicated a prevalence of 15% (10/64) of the Thr-76 mutant allele against 62% (40/64) of the Lys-76 wild-type allele. No mutation of the allele Thr-76 was observed at Anonkoua Kouté while this mutant allele was in 31% (5/16) and 25% (5/20) of isolate sequences from Port-Bouët and Ayamé respectively. Conclusion More than a decade after the discontinuation of the use of chloroquine in Côte d’Ivoire, the proportion of parasites sensitive to this anti-malarial seems to increase in Anonkoua-kouté, Port-bouët and Ayamé

Genetic Variants Assessing Crohn’s Disease Pattern in Pediatric Inflammatory Bowel Disease Patients by a Clinical Exome Survey

12Background: Inflammatory bowel diseases (IBDs) are complex, multifactorial disorders that comprise Crohn’s disease (CD) and ulcerative colitis (UC). Recent discoveries have brought much attention to the genetic predisposition of patients with IBD. Here we evaluate the interaction between IBD genetic risk factors susceptibility and CD occurrence in an IBD pediatric patient population, performing a clinical exome survey. Methods: From February 2018 to April 2019, we collected blood samples from 7 pediatric patients with IBD concerns from several collaborating health centers and/or hospitals. Blood samples were processed by extracting and sequencing DNA for a clinical exome survey. Shophia-DDM-v3-4 platform allowed sequenced reads alignment on hg19 genome as well as genetic variant calling. Both IBD risk and pathogenic genetic variants covered by at least 20 reads were selected for subjacent analysis. Results: Normality and Bartlett tests of both risk and pathogenic genetic variants suggested random and heterogeneous distribution of these variants in this group of IBD pediatric patients. P value clustering analysis by processing 157 IBD risk factors revealed genetic heterogeneity in IBD population and suggested two pathways influencing IBD development. In particular, (1) genetic variants associated with autoimmune and (2) metabolic diseases and CD risk factors (rs2066844 and rs2241880 single nucleotide polymorphism variants, respectively, of genes NOD2 and ATG16L) were identified in distinct clusters of IBD patients (P <.05). Moreover, the heterogeneous distribution of the following variants rs10065172 (IRGM), rs1805010 (IL4R), rs5030737 (MBL2), and rs33995883 (LRRK2) in this group of IBD patients was consistent with their random distribution in that population. Conclusion: Our study revealed specific genetic variants linked to CD susceptibility, autoimmune and/or innate immunodeficiency as well as to metabolic defects, as favoring factors of IBD, suggesting the valuable role of next generation sequencing (NGS) approaches in IBD molecular diagnostic procedures.openopenDago Dougba Noel, Pinelli Marinella, Giacomelli Mauro, Serena Ilaria Tripodi, Alessia Pin, Arrigo Serena, Bramuzzo Matteo, Fuoti Maurizio Giuseppe, Alvisi Patrizia, Calza Stefano, Alberto Tommasini, Badolato RaffaeleDago Dougba Noel, ; Pinelli, Marinella; Giacomelli, Mauro; Serena Ilaria Tripodi, ; Pin, Alessia; Arrigo, Serena; Bramuzzo, Matteo; Fuoti Maurizio Giuseppe, ; Alvisi, Patrizia; Calza, Stefano; Tommasini, Alberto; Badolato, Raffael

Estrogen receptor beta impacts hormone-induced alternative mRNA splicing in breast cancer cells.

BackgroundEstrogens play an important role in breast cancer (BC) development and progression; when the two isoforms of the estrogen receptor (ERα and ERβ) are co-expressed each of them mediate specific effects of these hormones in BC cells. ERβ has been suggested to exert an antagonist role toward the oncogenic activities of ERα, and for this reason it is considered an oncosuppressor. As clinical evidence regarding a prognostic role for this receptor subtype in hormone-responsive BC is still limited and conflicting, more knowledge is required on the biological functions of ERβ in cancer cells. We have previously described the ERβ and ERα interactomes from BC cells, identifying specific and distinct patterns of protein interactions for the two receptors. In particular, we identified factors involved in mRNA splicing and maturation as important components of both ERα and ERβ pathways. Guided by these findings, here we performed RNA sequencing to investigate in depth the differences in the early transcriptional events and RNA splicing patterns induced by estradiol in cells expressing ERα alone or ERα and ERβ.ResultsExon skipping was the most abundant splicing event in the post-transcriptional regulation by estradiol. We identified several splicing events induced by ERα alone and by ERα+ERβ, demonstrating for the first time that ERβ significantly affects estrogen-induced splicing in BC cells, as revealed by modification of a subset of ERα-dependent splicing by ERβ, as well as by the presence of splicing isoforms only in ERβ+cells. In particular, we observed that ERβ+BC cell lines exhibited around 2-fold more splicing events than the ERβ- cells. Interestingly, we identified putative direct targets of ERβ-mediated alternative splicing by correlating the genomic locations of ERβ and ERα binding sites with estradiol-induced differential splicing in the corresponding genes.ConclusionsTaken together, these results demonstrate that ERβ significantly affects estrogen-induced early transcription and mRNA splicing in hormone-responsive BC cells, providing novel information on the biological role of ERβ in these tumors

Estrogen receptor beta impacts hormone-induced alternative mRNA splicing in breast cancer cells

Estrogens play an important role in breast cancer (BC) development and progression; when the two isoforms of the estrogen receptor (ERα and ERβ) are co-expressed each of them mediate specific effects of these hormones in BC cells. ERβ has been suggested to exert an antagonist role toward the oncogenic activities of ERα, and for this reason it is considered an oncosuppressor. As clinical evidence regarding a prognostic role for this receptor subtype in hormone-responsive BC is still limited and conflicting, more knowledge is required on the biological functions of ERβ in cancer cells. We have previously described the ERβ and ERα interactomes from BC cells, identifying specific and distinct patterns of protein interactions for the two receptors. In particular, we identified factors involved in mRNA splicing and maturation as important components of both ERα and ERβ pathways. Guided by these findings, here we performed RNA sequencing to investigate in depth the differences in the early transcriptional events and RNA splicing patterns induced by estradiol in cells expressing ERα alone or ERα and ERβ