9 research outputs found
Effects of Prostaglandin E1 in the genesis of blood capillaries in the ischemic skeletal muscle of rats: histological study and ultra-structural analysis
Objetivo: o objetivo deste trabalho foi estudar os aspectos morfológicos e ultra-estruturais na
gênese de capilares sanguíneos em músculo esquelético do membro caudal de ratos
submetidos à isquemia sob a ação da Prostaglandina E1 (PGE1), administrada por via
intramuscular ou endovenosa. Métodos: foram utilizados 60 ratos (Rattus norvegicus
albinus), linhagem Wistar-UEM, distribuídos aleatoriamente em três grupos de 20,
redistribuídos igualmente em dois subgrupos, observados no 7o
e 14o
dias, sendo um grupo
controle onde apenas foi provocada a isquemia no membro, outro com a isquemia e a injeção
da PGE1 via intramuscular (IM), e outro com a isquemia e a injeção da PGE1 endovenosa
(EV). Para análise dos resultados, foram realizadas a coloração com hematoxilina & eosina
(HE), a imuno-histoquímica e a microscopia eletrônica de transmissão (MET). Resultados:
constatou-se um aumento estatisticamente significante no número de capilares nos subgrupos
com o uso da PGE1 IM e EV, através da contagem nos cortes corados com HE. Houve
marcação de capilares e vasos de maior calibre nestes mesmos subgrupos, porém, esta reação
não foi eficiente para a quantificação dos capilares. Na MET encontraram-se evidências de
formação de novos capilares. Conclusões: a PGE1, administrada por via IM ou EV,
promoveu, após 14 dias de observação, um aumento no número de capilares no músculo
esquelético de ratos submetido à isquemia, identificáveis histologicamente com a coloração
em HE. Na análise ultra-estrutural encontraram-se alterações que sugerem, nos animais sob a
ação da PGE1, que a neoformação vascular possa ter ocorrido por angiogênese e
vasculogênese. A imuno-coloração, apesar da marcação de capilares e vasos maiores, não
permitiu estabelecer uma correlação com o aumento de vasos encontrados na coloração com
HE.Objective: The objective of this work was to study the morphologic and ultra-structural
aspects in the genesis of blood capillaries in the lower limb skeletal muscle of rats submitted
to ischemia under the action of intramuscular or endovenous Prostaglandin E1 (PGE1).
Methods: Sixty Wistar-UEM rats (Rattus norvegicus albinus) were used, randomly
distributed into three groups of 20, equally redistributed into two subgroups, observed at the
7th and 14th days as follows: group only with ischemia was considered as control (C), group
with ischemia and intramuscular injection of PGE1 (IM), and group with ischemia and
endovenous injection of the PGE1 (EV). The analysis of results was performed with the HE
staining, imuno-histochemistry and transmission electronic microscopy (TEM). Results: an
increase statistically significant was verified in the number of capillaries in the subgroups
with PGE1 using IM or EV, through the counting in the samples with HE staining.
Demarcation of capillaries and larger vases in these subgroups were observed, however, this
reaction was not efficient for the quantification of the capillaries. In the electronic
microscopy, evidences of new capillary formation were found. Conclusions: intramuscular or
endovenous PGE1 promoted an increase on the number of capillaries in the skeletal muscle of
rats submitted to ischemia after 14 days of observation histologically identifiable through HE
staining. In the ultra-structural analysis, alterations found suggest, for animals under the
action of PGE1 that the vascular neoformation might have occurred through angiogenesis
and/or vasculogenesis. The imuno-staining, despite the demarcation of capillary and larger
vases, did not allow the establishment of a correlation with the increase of vases found in the
HE staining
Glutamine dipeptide supplementation improves clinical responses in patients with diabetic foot syndrome
The effect of glutamine dipeptide (GDP) supplementation in patients with diabetic foot syndrome was evaluated. A total of 22 patients took part in the study. GDP was supplied in 10 g sachets, and was dissolved in water immediately before use, with ingestion once a day, after lunch or after dinner (20 g/day) over a period of 30 days. Quantification of foot insensitive areas, oxidative stress, blood cytokines, and biochemical, hematological and toxicological parameters was performed before and after GDP supplementation. We observed an increase in blood levels of interferon-α (P=0.023), interferon-γ (P=0.038), interleukin-4 (P=0.003), interleukin-6 (P=0.0025), interleukin-7 (P=0.028), interleukin-12 p40 (P=0.017), interleukin-13 (P=0.001), leukocytes (P=0.037), eosinophils (P=0.049), and typical lymphocytes (
Glutamine dipeptide supplementation improves clinical responses in patients with diabetic foot syndrome
ABSTRACT The effect of glutamine dipeptide (GDP) supplementation in patients with diabetic foot syndrome was evaluated. A total of 22 patients took part in the study. GDP was supplied in 10 g sachets, and was dissolved in water immediately before use, with ingestion once a day, after lunch or after dinner (20 g/day) over a period of 30 days. Quantification of foot insensitive areas, oxidative stress, blood cytokines, and biochemical, hematological and toxicological parameters was performed before and after GDP supplementation. We observed an increase in blood levels of interferon-α (P=0.023), interferon-γ (P=0.038), interleukin-4 (P=0.003), interleukin-6 (P=0.0025), interleukin-7 (P=0.028), interleukin-12 p40 (P=0.017), interleukin-13 (P=0.001), leukocytes (P=0.037), eosinophils (P=0.049), and typical lymphocytes (P<0.001) due to GDP administration. In addition, we observed a reduced number (P=0.048) of insensitive areas on the foot, and reduction (P=0.047) of fasting hyperglycemia. Patients also showed increased blood high density lipoprotein (P<0.01) and protein thiol groups (P=0.004). These favorable results were associated with the absence of renal and hepatic toxicity. These results are of clinical relevance, since supplementation with GDP over 30 days improved clinical responses in patients with diabetic foot syndrome
Avaliação do número de alvéolos de pulmões remanescentes de ratos após lobectomia ou bilobectomia Evaluation of the number of alveoli of the remaining rat lungs after lobectomy or bilobectomy
Realizou-se este estudo com objetivo de analisar a resposta adaptativa da ressecção pulmonar, tendo-se como parâmetro a histologia do parênquima pulmonar remanescente. Foram utilizados quarenta e cinco <em>Rattus novergicus albinus</em>, Wistar, fêmeas, adultos. Distribuídos em grupo I, animais submetidos a lobectomia média direita, e redistribuídos em dois subgrupos para avaliação do pós-operatório aos 30 e 60 dias. No grupo II, os animais foram submetidos a bilobectomia cranial e média direita e também foram redistribuídos em subgrupos para posterior avaliação. Os animais que constituíram o grupo controle não sofreram intervenção cirúrgica. Os resultados mostraram que após a ressecção, ocorreu hiperplasia do parênquima pulmonar remanescente, além de dilatação dos espaços aéreos. Nos animais do grupo controle, o número de alvéolos foi sempre maior quando comparados aos animais submetidos à ressecção pulmonar<br>The aim of this study was to analyze the lung resection adaptive response, using as a parameter the histology of the remaining pulmonary parenchyma. Forty-five adult female Wistar rats, <em>Rattus novergicus albinus</em>, were used distributed into group I, II. The group I animals underwent a right-medium lobectomy, and were re-arranged in two subgroups for post-surgical evaluation after 30 and 60 days, respectively. The group II animals underwent both a cranial and a medium-right bilobectomy, being re-arranged in sub-groups for subsequent evaluation. The control group animals did not undergo any surgical procedure. The results showed that there was a hyperplasia of the remaining pulmonary parenchyma after resection, besides the dilation of the aerial spaces. The number of alveoli in the control group animals was always higher when compared to the animals that experienced pulmonary resectio
Investigation of the use of regional heparinization during temporary arterial ischemia performed in rabbits
The purpose of this study is to evaluate the effect of the regional heparinization during temporary arterial ischemia performed in rabbits. The evaluated parameters served for measuring were activated partial thromboplastin times (aPTT) as well as the presence or absence of thrombi or endothelial edema in the analyzed vessels. Forty New Zealand rabbits were utilized. They were distributed into two groups of twenty animals. After dissection and repair of the left illiac artery, an arteriotomy was carried out and then a polyethylene catheter was fitted in so that heparin solution could be injected into the experimental group and physiological serum into the control group. The aPTT was measured at the beginning of the experiment and after ninety minutes of ischemia. There were no significant differences found from the initial values to the end of the experiment. Throughout microscopic evaluation the presence of thrombi in the vessels of the animals from both groups was not found. Intimal thickening as well as disruption of the middle layer regarded as endothelial edema ocurred in only one animal from the control group, however it was not considered a significant data. It was concluded that the injection of heparin (60 UI/kg weight) did not provoke significant alterations in the systemic coagulation of the rabbit. Furthermore, in the absence of endothelial lesion thrombosis of the analyzed vessels did not occur, neither with nor without the utilization of heparin.O objetivo deste trabalho foi avaliar o efeito da heparinização regional durante a isquemia arterial temporária em coelhos. Os parâmetros avaliados foram a medida do tempo de tromboplastina parcial ativada (TTPa) e a presença ou ausência de trombos ou edema endotelial nos vasos estudados. Foram utilizados 40 coelhos Nova Zelândia, distribuídos em dois grupos de 20 animais. Após dissecção e reparo da artéria ilíaca esquerda, realizou uma arteriotomia e introduziu-se um cateter de polietileno para injeção de solução de heparina no grupo experimento ou de soro fisiológico no grupo controle. O TTPa foi medido no início do experimento e após 90 minutos de isquemia, não sendo encontrado diferenças significantes entre os valores iniciais e ao final do experimento. Na avaliação microscópica não foi encontrada a presença de trombos nos vasos dos animais de ambos os grupos. Em apenas um animal do grupo controle houve a ocorrência de espessamento intimal e desarranjo da camada média, interpretado como edema endotelial, porém este dado não foi significante. Concluiu-se que a injeção intra-arterial de heparina na dose de 60 UI/kg peso não provocou alterações significantes na coagulação sistêmica do coelho, e que na ausência de lesão endotelial não ocorreu trombose dos vasos estudados, com ou sem a utilização de heparina.Universidade Estadual de Maringá (UEM)UNIFESPEPMUEMUNIFESPEPMSciEL
INVESTIGAÇÃO DO USO DE HEPARINIZAÇÃO REGIONAL DURANTE ISQUEMIA ARTERIAL TEMPORÁRIA EM COELHOS
O objetivo deste trabalho foi avaliar o efeito da heparinização regional durante a isquemia arterial temporária em coelhos. Os parâmetros avaliados foram a medida do tempo de tromboplastina parcial ativada (TTPa) e a presença ou ausência de trombos ou edema endotelial nos vasos estudados. Foram utilizados 40 coelhos Nova Zelândia, distribuídos em dois grupos de 20 animais. Após dissecção e reparo da artéria ilíaca esquerda, realizou uma arteriotomia e introduziu-se um cateter de polietileno para injeção de solução de heparina no grupo experimento ou de soro fisiológico no grupo controle. O TTPa foi medido no início do experimento e após 90 minutos de isquemia, não sendo encontrado diferenças significantes entre os valores iniciais e ao final do experimento. Na avaliação microscópica não foi encontrada a presença de trombos nos vasos dos animais de ambos os grupos. Em apenas um animal do grupo controle houve a ocorrência de espessamento intimal e desarranjo da camada média, interpretado como edema endotelial, porém este dado não foi significante. Concluiu-se que a injeção intra-arterial de heparina na dose de 60 UI/kg peso não provocou alterações significantes na coagulação sistêmica do coelho, e que na ausência de lesão endotelial não ocorreu trombose dos vasos estudados, com ou sem a utilização de heparina
Glutamine dipeptide supplementation improves clinical responses in patients with diabetic foot syndrome
ABSTRACT The effect of glutamine dipeptide (GDP) supplementation in patients with diabetic foot syndrome was evaluated. A total of 22 patients took part in the study. GDP was supplied in 10 g sachets, and was dissolved in water immediately before use, with ingestion once a day, after lunch or after dinner (20 g/day) over a period of 30 days. Quantification of foot insensitive areas, oxidative stress, blood cytokines, and biochemical, hematological and toxicological parameters was performed before and after GDP supplementation. We observed an increase in blood levels of interferon-α (P=0.023), interferon-γ (P=0.038), interleukin-4 (P=0.003), interleukin-6 (P=0.0025), interleukin-7 (P=0.028), interleukin-12 p40 (P=0.017), interleukin-13 (P=0.001), leukocytes (P=0.037), eosinophils (P=0.049), and typical lymphocytes (P<0.001) due to GDP administration. In addition, we observed a reduced number (P=0.048) of insensitive areas on the foot, and reduction (P=0.047) of fasting hyperglycemia. Patients also showed increased blood high density lipoprotein (P<0.01) and protein thiol groups (P=0.004). These favorable results were associated with the absence of renal and hepatic toxicity. These results are of clinical relevance, since supplementation with GDP over 30 days improved clinical responses in patients with diabetic foot syndrome