A comparative study of genotoxicity and oxidative stress before and after using lemon balm and cinnamon in subjects exposed to Nickel Welding Fumes

     By producing reactive oxygen and nitrogen species, metal-induced toxicity and carcinogenicity alter DNA bases, increase  lipid peroxidation, and change calcium and sulfhydryl homeostasis. The purpose of the study was to investigate putative effects of Lemon balm and Cinnamomum zeylanicum on the blood markers of welding workers. We measured nickel and chromium levels, biochemical parameters, blood oxidative stress markers, total antioxidant capacity (TAC), Myeloperoxidase (MPO), lipid peroxidation (LPO), and DNA damage. The study was conducted on 55 male workers who worked in an industrial subjects exposed to Nickel Welding Fumes. The participants were administered Lemon balm and Cinnamon extract infusion 1.5 (0.12 extract) and 0.25(0.013 extract) g/100 mL, respectively, they have drunken twice a day for 30 days at 7:00 AM and 2:00 PM every day. In order to analyze the achieved data, paired t-test and Pearson correlation coefficient have been used. After using the extract infusion, a significant increase revealed in TAC (p= 0.007). Also, administration of infusion decreased DNA damage but it was not statistically significant. After administration of infusion, a decrease in LPO and MPO were observed (p= 0.014, p=0.000 respectively). Also there is positive correlation between ALP and Ni with 8-oH-dG and also, between Ni and TAC. The results indicate that using infusion causes to raise in a TAC and reduce in DNA damage

Profile of micronucleus frequencies and DNA damage in different species of fish in a eutrophic tropical lake

Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species (Geophagus brasiliensis, Cichla temensis, Hoplias malabaricus, Astyanax bimaculatus lacustres, Oreochromis niloticus, Cyprinus carpio and Steindachnerina insculpita) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in Cichla temensis and Hoplias malabaricus, which were statistically significant when compared to the other species. However, Steindachnerina insculpita (a detritivorous and lake-floor feeder species) showed the highest index of DNA damage in the comet assay, followed by C. temensis (piscivorous). Nuclear abnormalities, such as binucleated, blebbed, lobed and notched cells, were used as evidence of cytotoxicity. Oreochromis niloticus followed by Hoplias malaricus, ominivorous/detritivotous and piscivorous species, respectively, presented the highest frequency of nuclear abnormalities, especially notched cells, while the herbivorous Astyanax bimaculatus lacustres showed the lowest frequency compared to the other species studied. Thus, for biomonitoring aquatic genotoxins under field conditions, the food web should also be considered

Cobalt-vitamin B-12 interrelationships in liver of fetuses and infants

mean +-SEM). The percentage of cobalt as vitamin B-12 was not affected by fetal development measured as liver weight (r = 0.08; P < 0.4). Liver weight was not significantly correlated with concentrations of either cobalt . However. the correlation between cobalt and vitamin B-12 concentrations was highly significant (r = 0.83; P < 0.000