134 research outputs found
The effect of fat supplementation of concentrates on digestion and utilization of energy by productive dairy cows.
Two energy balance experiments each used 6 high-yielding dairy cows. In the first, a 3 X 3 Latin square with one replicate, rations were of 7 kg hay and 11 to 14 kg of concentrates having crude protein 251 g and gross energy 17.9 MJ/kg, given alone or with 5% tallow or 5% soya bean oil. Three weeks of adaptation were followed by 10 to 12 days when excreta were collected and gas exchange estimated. Digestibility and metabolizable energy of concentrates and hay were also assessed using wethers fed to maintenance only. Intakes of DM and digestibility of nutrients other than lipid did not differ among treatments with either sheep or cows. There were no apparent differences in rumen fermentation in the one cannulated cow used, and energy loss in urine was also unaffected. Lipid supplement reduced methane loss. Milk yield and milk energy were increased with tallow, with a simultaneous reduction of milk fat and percentage of protein. Soya bean oil reduced the percentage of milk fat and milk energy produced. The second trial, using similar animals and with similar management had a change-over design in which the concentrate was given alone or with 7% tallow. Results confirmed those of trial 1. The effect of level of feeding on the efficiency of utilization of energy is discussed. (Abstract retrieved from CAB Abstracts by CABI’s permission
Further studies on the effect of fat supplementation of concentrates fed to lactating dairy cows. II. Total digestion and energy utilization.
For wether sheep given 300 g hay and 800 g concentrates the addition of fat (beef tallow:palm kernel oil 9:1) to concentrates at 7 or 12% tended to decrease crude fibre digestibility and increased crude fat digestibility. For 2 rumen-fistulated cows given concentrates containing 7% fat, or 12% fat free or adsorbed on carrier, and 2 cows with fistulae and reentrant duodenal cannulae given concentrates with 12% fat in rations with hay:concentrate 1:2, there were no significant differences between diets in the digestibility of any nutrient; fat digestibility was decreased at feed intakes above maintenance, particularly with the 12% fat concentrates. The use of a carrier in the 12% fat concentrates did not affect nutrient digestibilities for sheep or cows. Inclusion of fat in concentrates increased the metabolizability of sheep diets but there were no significant differences among cattle diets, although methane losses tended to decrease as fat content increased. Additional energy from fat tended to be retained rather than used for milk yield. ME utilization for milk, storage and maintenance was 61-62.5%, tending to be highest with the 12% adsorbed fat. [See DSA 45, 5398 for part I.] (Abstract retrieved from CAB Abstracts by CABI’s permission
Extremely thin layer plastification for focused‐ion beam scanning electron microscopy: an improved method to study cell surfaces and organelles of cultured cells
Separate Pathways for Antigen Presentation by CD1 Molecules
AbstractThe ability to sample relevant intracellular compartments is necessary for effective antigen presentation. To detect peptide antigens, MHC class I and II molecules differentially sample cytosolic and endosomal compartments. CD1 constitutes another lineage of lipid antigen-presenting molecules. We show that CD1b traffics deeply into late endosomal compartments, while CD1a is excluded from these compartments and instead traffics independently in the recycling pathway of the early endocytic system. Further, CD1b but not CD1a antigen presentation is dependent upon vesicular acidification. Since lipids and various bacteria are known to traffic differentially, either penetrating deeply into the endocytic system or following the route of recycling endosomes, these findings elucidate efficient monitoring of distinct components of the endocytic compartment by CD1 lipid antigen-presenting molecules
Response Functions to Critical Shocks in Social Sciences: An Empirical and Numerical Study
We show that, provided one focuses on properly selected episodes, one can
apply to the social sciences the same observational strategy that has proved
successful in natural sciences such as astrophysics or geodynamics. For
instance, in order to probe the cohesion of a policy, one can, in different
countries, study the reactions to some huge and sudden exogenous shocks, which
we call Dirac shocks. This approach naturally leads to the notion of structural
(as opposed or complementary to temporal) forecast. Although structural
predictions are by far the most common way to test theories in the natural
sciences, they have been much less used in the social sciences. The Dirac shock
approach opens the way to testing structural predictions in the social
sciences. The examples reported here suggest that critical events are able to
reveal pre-existing ``cracks'' because they probe the social cohesion which is
an indicator and predictor of future evolution of the system, and in some cases
foreshadows a bifurcation. We complement our empirical work with numerical
simulations of the response function (``damage spreading'') to Dirac shocks in
the Sznajd model of consensus build-up. We quantify the slow relaxation of the
difference between perturbed and unperturbed systems, the conditions under
which the consensus is modified by the shock and the large variability from one
realization to another
Trafficking of prion proteins through a caveolae-mediated endosomal pathway
To understand the posttranslational conversion of the cellular prion protein (PrPC) to its pathologic conformation, it is important to define the intracellular trafficking pathway of PrPC within the endomembrane system. We studied the localization and internalization of PrPC in CHO cells using cryoimmunogold electron microscopy. At steady state, PrPC was enriched in caveolae both at the TGN and plasma membrane and in interconnecting chains of endocytic caveolae. Protein A–gold particles bound specifically to PrPC on live cells. These complexes were delivered via caveolae to the pericentriolar region and via nonclassical, caveolae-containing early endocytic structures to late endosomes/lysosomes, thereby bypassing the internalization pathway mediated by clathrin-coated vesicles. Endocytosed PrPC-containing caveolae were not directed to the ER and Golgi complex. Uptake of caveolae and degradation of PrPC was slow and sensitive to filipin. This caveolae-dependent endocytic pathway was not observed for several other glycosylphosphatidyl inositol (GPI)-anchored proteins. We propose that this nonclassical endocytic pathway is likely to determine the subcellular location of PrPC conversion
Consumer evaluation of complaint handling in the Dutch health insurance market
<p>Abstract</p> <p>Background</p> <p>How companies deal with complaints is a particularly challenging aspect in managing the quality of their service. In this study we test the direct and relative effects of service quality dimensions on consumer complaint satisfaction evaluations and trust in a company in the Dutch health insurance market.</p> <p>Methods</p> <p>A cross-sectional survey design was used. Survey data of 150 members of a Dutch insurance panel who lodged a complaint at their healthcare insurer within the past 12 months were surveyed. The data were collected using a questionnaire containing validated multi-item measures. These measures assess the service quality dimensions consisting of functional quality and technical quality and consumer complaint satisfaction evaluations consisting of complaint satisfaction and overall satisfaction with the company after complaint handling. Respondents' trust in a company after complaint handling was also measured. Using factor analysis, reliability and validity of the measures were assessed. Regression analysis was used to examine the relationships between these variables.</p> <p>Results</p> <p>Overall, results confirm the hypothesized direct and relative effects between the service quality dimensions and consumer complaint satisfaction evaluations and trust in the company. No support was found for the effect of technical quality on overall satisfaction with the company. This outcome might be driven by the context of our study; namely, consumers get in touch with a company to resolve a specific problem and therefore might focus more on complaint satisfaction and less on overall satisfaction with the company.</p> <p>Conclusions</p> <p>Overall, the model we present is valid in the context of the Dutch health insurance market. Management is able to increase consumers' complaint satisfaction, overall satisfaction with the company, and trust in the company by improving elements of functional and technical quality. Furthermore, we show that functional and technical quality do not influence consumer satisfaction evaluations and trust in the company to the same extent. Therefore, it is important for managers to be aware of the type of consumer satisfaction they are measuring when evaluating the handling of complaints within their company.</p
Extracellular matrix formation after transplantation of human embryonic stem cell-derived cardiomyocytes
Transplantation of human embryonic stem cell-derived cardiomyocytes (hESC-CM) for cardiac regeneration is hampered by the formation of fibrotic tissue around the grafts, preventing electrophysiological coupling. Investigating this process, we found that: (1) beating hESC-CM in vitro are embedded in collagens, laminin and fibronectin, which they bind via appropriate integrins; (2) after transplantation into the mouse heart, hESC-CM continue to secrete collagen IV, XVIII and fibronectin; (3) integrin expression on hESC-CM largely matches the matrix type they encounter or secrete in vivo; (4) co-transplantation of hESC-derived endothelial cells and/or cardiac progenitors with hESC-CM results in the formation of functional capillaries; and (5) transplanted hESC-CM survive and mature in vivo for at least 24 weeks. These results form the basis of future developments aiming to reduce the adverse fibrotic reaction that currently complicates cell-based therapies for cardiac disease, and to provide an additional clue towards successful engraftment of cardiomyocytes by co-transplanting endothelial cells
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