Measurements of nitric oxide and ammonia soil fluxes from a wet savanna ecosystem site in West Africa during the DACCIWA field campaign

Biogenic fluxes from soil at a local and regional scale are crucial to study air pollution and climate. Here we present field measurements of soil fluxes of nitric oxide (NO) and ammonia (NH3) observed over four different land cover types, i.e. bare soil, grassland, maize field, and forest, at an inland rural site in Benin, West Africa, during the DACCIWA field campaign in June and July 2016. At the regional scale, urbanization and a massive growth in population in West Africa have been causing a strong increase in anthropogenic emissions. Anthropogenic pollutants are transported inland and northward from the megacities located on the coast, where the reaction with biogenic emissions may lead to enhanced ozone production outside urban areas, as well as secondary organic aerosol formation, with detrimental effects on humans, animals, natural vegetation, and crops. We observe NO fluxes up to 48.05 ngNm 2 s 1. NO fluxes averaged over all land cover types are 4:79 5:59 ngNm 2 s 1, and maximum soil emissions of NO are recorded over bare soil. NH3 is dominated by deposition for all land cover types. NH3 fluxes range between 6:59 and 4.96 ngNm 2 s 1. NH3 fluxes averaged over all land cover types are 0:911:27 ngNm 2 s 1, and maximum NH3 deposition is measured over bare soil. The observations show high spatial variability even for the same soil type, same day, and same meteorological conditions. We compare point daytime average measurements of NO emissions recorded during the field campaign with those simulated by GEOS-Chem (Goddard Earth Observing System Chemistry Model) for the same site and find good agreement. In an attempt to quantify NO emissions at the regional and national scale, we also provide a tentative estimate of total NO emissions for the entire country of Benin for the month of July using two distinct methods: upscaling point measurements and using the GEOS-Chem model. The two methods give similar results: 1:170:6 and 1.44 GgN month 1, respectively. Total NH3 deposition estimated by upscaling point measurements for the month of July is 0.21 GgN month1

Th17 Cells Are Involved in the Local Control of Tumor Progression in Primary Intraocular Lymphoma

BACKGROUND: Th17 cells play an important role in the pathogenesis of many autoimmune diseases, but despite some reports of their antitumor properties, too little is known about their presence and role in cancers. Specifically, knowledge is sparse about the relation of Th17 to lymphoma microenvironments and, more particularly, to the microenvironment of primary intraocular B-cell lymphoma (PIOL), an aggressive lymphoma with a poor prognosis. METHODS AND PRINCIPAL FINDINGS: In this work, we investigated the presence of Th17 cells and their related cytokines in a syngeneic model of PIOL, a subtype of non-Hodgkin lymphoma. The very small number of lymphocytes trafficking in normal eyes, which represent a low background as compared to tumor-bearing eyes, allows us to develop the present model to characterize the different lymphocyte subsets present when a tumor is developing. IL-21 mRNA was expressed concomitantly with IL-17 mRNA in tumor-bearing eyes and intracellular expression of IL-17A and IL-21 in infiltrating CD4(+) T lymphocytes. Interestingly, IL-17A production by T cells was negatively correlated with tumor burden. We also showed that IL-21 but not IL-17 inhibits tumor cell proliferation in vitro. CONCLUSIONS: These data demonstrate that IL-17A and IL-21-producing CD4(+) T cells, referred as Th17 cells, infiltrate this tumor locally and suggest that Th17-related cytokines may counteract tumor progression via IL-21 production. Thus, Th17 cells or their related cytokines could be considered to be a new therapeutic approach for non-Hodgkin B-cell lymphomas, particularly those with an ocular localization

Estimation of the Global Solar Radiation Received on the Soil in Nalohou and Natitingou (Northern Benin)

The design of solar energy systems requires knowledge of the global solar radiation received by solar panels on the ground. However, for many developing countries such as the Benin Republic, there is a serious lack of radiometric stations everywhere in the country. The unavailability of these data, therefore, limits knowledge regarding the optimal functioning of solar installations. To cope with these difficulties, a method for estimating the global irradiation is developed at Nalohou AMMA CATCH station and Natintingou synoptic station, two sites in North-West Benin. This model depends on meteorological and astronomical parameters. Meteorological data such as sunshine duration, relative humidity, and air temperature have been collected on the synoptic station of Natitingou, and observed data of irradiation are collected from Nalohou. They are used to validate the model. The results show that the estimation of the mean square deviation value is around 10 -2 . Overall solar radiation is significant in this region and its annual average value varies between 9.80 kWh/m2 and 10.50 kWh/m2. This model can, therefore, be used by solar system operators to optimize energy production in the region

Vertical Profile of Wind Diurnal Cycle in the Surface Boundary Layer over the Coast of Cotonou, Benin, under a Convective Atmosphere

The characteristics of the wind vertical profile over the coast of Cotonou during wind convective diurnal cycle were explored in this study. Wind data at 10 m above the ground and the radiosonde data in the lower 60 m of the surface boundary layer were used over the period from January 2013 to December 2016. Based on Monin–Obukhov theory, the logarithmic and power laws have allowed characterizing the wind profile. The error estimators of the Root Mean Square Error (RMSE) and the Mean Absolute Error (MAE) were, respectively, evaluated at 0.025; 0.016 (RMSE; MAE) and 0.018; 0.015. At the site of Cotonou, the atmosphere is generally unstable from 09:00 to 18:00 MST and stable for the remainder of the time. The annual mean value of the wind shear coefficient is estimated at 0.20 and that of the ground surface roughness length and friction velocity are, respectively, of 0.007 m, 0.38 m·s−1. A comparative study between the wind extrapolation models and the data was carried out in order to test their reliability on our study site. The result of this is that whatever the time of the year is, only the models proposed (best fitting equation) are always in good agreement with the data unlike the other models evaluated. Finally, from the models suitable for our site, the profile of wind convective diurnal cycle was obtained by extrapolation of the wind data measured at 10 m from the ground. The average wind speed during this cycle is therefore evaluated to 8.07 m·s−1 for August which is the windiest month and to 4.98 m·s−1 for the least windy month (November) at 60 m of the ground. Considering these results, we can so consider that the site of Cotonou coastal could be suitable for the installation of wind turbines

Naturally occurring CD4+CD25+Foxp3+ regulatory T cells participate in but do not govern immune escape in primary intraocular lymphoma

International audienc

Surface atmosphere exchanges of chemical compounds in a wet savanna ecosystem site in West Africa during the DACCIWA field campaign

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Preclinical study of Ublituximab, a Glycoengineered anti-human CD20 antibody, in murine models of primary cerebral and intraocular B-cell lymphomas

International audiencePURPOSE: Primary cerebral lymphoma (PCL) and primary intraocular lymphoma (PIOL) belong to the systemic diffuse large B-cell lymphoma family and are characterized by the presence of CD20(+) lymphoma B cells in the brain or the eye. These highly aggressive malignancies have a poor prognosis and no specific therapy. The presence of effector immune cells in the damaged brain and vitreous suggests that treatment with anti-human CD20 (hCD20) monoclonal antibodies might be effective. We developed murine models of PCL and PIOL to assess the intracerebral and intraocular antitumor effect of ublituximab, a promising glycoengineered anti-hCD20 mAb with a high affinity for FcgammaRIIIa (CD16) receptors. METHODS: The murine lymphoma B-cell line A20.IIA-GFP-hCD20 (H-2(d)) was injected into the right cerebral striatum or the vitreous of immunocompetent adult BALB/c mice (H-2(d)). Four to 7 days later, ublituximab was injected intracerebrally or intravitreously into the tumor site. Rituximab was the reference compound. Survival was monitored for injected mice; histopathological and flow cytometric analyses were performed to study tumor growth and T-cell infiltration. RESULTS: Single doses of ublituximab, injected intracerebrally or intravitreously, had a marked antitumor effect, more pronounced than that obtained with the same dose of rituximab in these conditions. The reduction in tumor cells was correlated with an increased proportion of CD8(+) T cells. This efficacy was observed only against lymphoma B cells expressing hCD20. CONCLUSIONS: These in vivo results confirm the potential of the glycoengineered anti-hCD20 mAb ublituximab as an innovative therapeutic approach to treat primary central nervous system lymphoma and other B-cell lymphomas