Tetra­kis(μ3-2-{[1,1-bis­(hydroxy­meth­yl)-2-oxidoeth­yl]imino­meth­yl}-6-methoxy­phenol­ato)tetra­nickel(II) tetra­hydrate

The title complex, [Ni4(C12H15NO4)4]·4H2O, has crystal­lographic fourfold inversion symmetry, with each NiII ion coordinated in a slightly distorted square-pyramidal coordination environment and forming an Ni4O4 cubane-like core. In the crystal structure, inter­molecular O—H⋯O hydrogen bonds connect complex and water mol­ecules to form a three-dimensional network. The O atom of one of the unique hydroxy­methyl groups is disordered over two sites, with the ratio of occupancies being approximately 0.79:0.21

Bis[μ-N-(3-meth­oxy-2-oxidobenzyl­idene-1:2κ2 O 2:O 2)-l-isoleucinato-2κ2 N,O]bis­(1,10-phenanthroline-1κ2 N,N′)dinickel(II) methanol tetra­solvate trihydrate

In the title complex, [Ni2(C14H17NO4)2(C12H8N2)2]·4CH3OH·3H2O, the two NiII ions are bridged by two Schiff base anions, leading to a dinuclear complex. One NiII ion is six-coordinated by four O atoms and two N atoms of two tridentate Schiff base ligands derived from the condensation of l-isoleucine and o-vanillin. The other NiII ion is six-coordinated by four N atoms of two 1,10-phenanthroline ligands and two O atoms of the Schiff base ligands. In the crystal, inter­molecular O—H⋯O and C—H⋯O hydrogen bonds lead to a three-dimensional structure. Intra­molecular C—H⋯O hydrogen bonds are also present. One of the methyl groups of the l-isoleucinate moieties is disordered over two sets of sites with an occupancy ratio of 0.687 (19):0.313 (19) and two methanol mol­ecules are half-occupied

[N-(3-Meth­oxy-2-oxidobenzyl­idene-κO 2)threoninato-κ2 O 1,N](1,10-phenanthroline-κ2 N,N′)copper(II) hemihydrate

In the title complex, [Cu(C12H13NO5)(C12H8N2)]·0.5H2O, the CuII ion is five-coordinated by one N atom and two O atoms from a tridentate Schiff base ligand, derived from the condensation of l-threonine and o-vanillin, and two N atoms from a 1,10-phenanthroline ligand in a distorted square-pyramidal geometry. In the crystal, inter­molecular O—H⋯O hydrogen bonds form a one-dimensional left-handed helical structureextending parallel to [001]. The water molecule of crystallization shows half-occupancy

{2-(4-Hy­droxy­phen­yl)-2-[(3-meth­oxy-2-oxidobenzyl­idene)amino-κ2 O 2,N]propanoato-κO}(1,10-phenanthroline-κ2 N,N′)copper(II) dihydrate

In the title complex, [Cu(C17H15NO5)(C12H8N2)]·2H2O, the central CuII ion is five-coordinate, bound to one N atom and two O atoms from the Schiff base ligand and by two N atoms from a 1,10-phenanthroline ligand in a distorted square-pyramidal configuration. In the crystal, inter­molecular O—H⋯O and C—H⋯O hydrogen bonds form a two-dimensional network parallel to (001)

Direct deposition of anatase TiO2 on thermally unstable gold nanobipyramid: Morphology-conserved plasmonic nanohybrid for combinational photothermal and photocatalytic cancer therapy

Deposition of crystalline titanium dioxide (TiO2) on gold nanostructures has been considered as a promising strategy for near-infrared (NIR) light-activated photocatalysis. A typical route comprises pre-deposition of amorphous TiO2 on the gold surface and its ensuing crystallization by high-temperature annealing. Such condition, however, is not compatible with highly plasmonic but thermally unstable sharp-tipped gold nanostructures, causing structural disruption and plasmonic decline. Herein, we report a hybridization method excluding high-temperature annealing, i.e., direct deposition of anatase TiO2 onto sharp-tipped gold nanobipyramid (Au NBP/a-TiO2) with conserving their morphology without agglomeration via low-temperature hydrothermal reaction. In addition to keeping the plasmonic photothermal performance, Au NBP/a-TiO2 exhibits enhanced photocatalytic generation of reactive oxygen species in response to the NIR excitation, evidencing the efficient injection of hot electrons from the Au NBP to the anatase shell. In vitro and in vivo studies revealed that the efficient photocatalytic/photothermal responses of Au NBP/a-TiO2, along with dispersion stability in biological media and minimal toxicity, hold potential for synergistic photothermal and photodynamic therapy. We believe that the low-temperature synthetic method introduced here might offer a general way of crystalline deposition of TiO2 on a variety of gold nanostructures, broadening the spectrum of NIR-responsive photocatalytic hybrid nanostructures for biomedical applications

The first clinical data of the SAPIEN 3 aortic valve in the treatment of aortic stenosis in China

BackgroundData on outcomes following transcatheter aortic valve replacement with SAPIEN 3 in China is limited as it was approved by the National Medical Products since 2020. The present study was designed to collect clinical data on the SAPIEN 3 aortic valve in Chinese patients with bicuspid aortic valve and tricuspid aortic valve stenosis.MethodsWe analyzed the patient characteristics, procedural features and procedural outcomes of the first 438 patients (223 for bicuspid aortic valve and 215 tricuspid aortic valve) from 21 provinces in 74 sites treated with the SAPIEN 3 valve system for transcatheter aortic valve replacement between September 2020 and May 2022.ResultsProcedural mortality was 0.7%. 5 cases during the operation were converted to surgery. Among 438 cases, permanent pacemaker implantation was performed in a total of 12 cases (2.7%). The patient had severe leaflet calcification of the aortic valve, with moderate and severe calcification reaching 39.7% and 35.2% respectively. The size of the implanted valves was predominantly 26 mm and 23 mm, reaching 42.5% and 39.5% respectively. The incidence of moderate or severe perivalvular leak in the postoperative period was 0.5%, with a predominance of 90/10 and 80/20 valve deployment height. There was a significant difference in the deployment height of the valve between bicuspid aortic valve and tricuspid aortic valve, with the bicuspid aortic valve having a more deployment height of 90/10. Annulus size in bicuspid aortic valve group was significantly larger than tricuspid aortic valve group. Valve sizing for oversized, within size, and undersized were different between bicuspid aortic valve and tricuspid aortic valve.ConclusionsProcedural success rates were high, with similar and good results for bicuspid aortic valve and tricuspid aortic valve, low perivalvular leak for both valve types, and low permanent pacemaker implantation rates for both valve types. Annulus size, valve sizing and coronary artery height were significantly different in the BAV and TAV group

Genomic Analyses Reveal Mutational Signatures and Frequently Altered Genes in Esophageal Squamous Cell Carcinoma

Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide and the fourth most lethal cancer in China. However, although genomic studies have identified some mutations associated with ESCC, we know little of the mutational processes responsible. To identify genome-wide mutational signatures, we performed either whole-genome sequencing (WGS) or whole-exome sequencing (WES) on 104 ESCC individuals and combined our data with those of 88 previously reported samples. An APOBEC-mediated mutational signature in 47% of 192 tumors suggests that APOBEC-catalyzed deamination provides a source of DNA damage in ESCC. Moreover, PIK3CA hotspot mutations (c.1624G>A [p.Glu542Lys] and c.1633G>A [p.Glu545Lys]) were enriched in APOBEC-signature tumors, and no smoking-associated signature was observed in ESCC. In the samples analyzed by WGS, we identified focal (<100 kb) amplifications of CBX4 and CBX8. In our combined cohort, we identified frequent inactivating mutations in AJUBA, ZNF750, and PTCH1 and the chromatin-remodeling genes CREBBP and BAP1, in addition to known mutations. Functional analyses suggest roles for several genes (CBX4, CBX8, AJUBA, and ZNF750) in ESCC. Notably, high activity of hedgehog signaling and the PI3K pathway in approximately 60% of 104 ESCC tumors indicates that therapies targeting these pathways might be particularly promising strategies for ESCC. Collectively, our data provide comprehensive insights into the mutational signatures of ESCC and identify markers for early diagnosis and potential therapeutic targets

Integrated Genomic Analysis of the Ubiquitin Pathway across Cancer Types

Protein ubiquitination is a dynamic and reversibleprocess of adding single ubiquitin molecules orvarious ubiquitin chains to target proteins. Here,using multidimensional omic data of 9,125 tumorsamples across 33 cancer types from The CancerGenome Atlas, we perform comprehensive molecu-lar characterization of 929 ubiquitin-related genesand 95 deubiquitinase genes. Among them, we sys-tematically identify top somatic driver candidates,including mutatedFBXW7with cancer-type-specificpatterns and amplifiedMDM2showing a mutuallyexclusive pattern withBRAFmutations. Ubiquitinpathway genes tend to be upregulated in cancermediated by diverse mechanisms. By integratingpan-cancer multiomic data, we identify a group oftumor samples that exhibit worse prognosis. Thesesamples are consistently associated with the upre-gulation of cell-cycle and DNA repair pathways, char-acterized by mutatedTP53,MYC/TERTamplifica-tion, andAPC/PTENdeletion. Our analysishighlights the importance of the ubiquitin pathwayin cancer development and lays a foundation fordeveloping relevant therapeutic strategies

Household, community, sub-national and country-level predictors of primary cooking fuel switching in nine countries from the PURE study

Introduction. Switchingfrom polluting (e.g. wood, crop waste, coal)to clean (e.g. gas, electricity) cooking fuels can reduce household air pollution exposures and climate-forcing emissions.While studies have evaluated specific interventions and assessed fuel-switching in repeated cross-sectional surveys, the role of different multilevel factors in household fuel switching, outside of interventions and across diverse community settings, is not well understood. Methods.We examined longitudinal survey data from 24 172 households in 177 rural communities across nine countries within the Prospective Urban and Rural Epidemiology study.We assessed household-level primary cooking fuel switching during a median of 10 years offollow up (∼2005–2015).We used hierarchical logistic regression models to examine the relative importance of household, community, sub-national and national-level factors contributing to primary fuel switching. Results. One-half of study households(12 369)reported changing their primary cookingfuels between baseline andfollow up surveys. Of these, 61% (7582) switchedfrom polluting (wood, dung, agricultural waste, charcoal, coal, kerosene)to clean (gas, electricity)fuels, 26% (3109)switched between different polluting fuels, 10% (1164)switched from clean to polluting fuels and 3% (522)switched between different clean fuels