Study on wound healing effect of low-carbon topical dressings with new green packaging

In order to verify the effect of the new green and low-carbon hydrogel dressing on promoting wound healing, this project applied a sodium alginate hydrogel dressing product containing Escherichia coli and taro toxin analgesic polypeptide (The specific ingredients of the dressing) to skin wounds in common rats. Effects of the hydrogel dressing on promoting skin wound healing was evaluated by observing the occurrence and frequency of behavioral changes in rats, observing wwhistological sections under a high-power microscope, changes in serum cytokine indicators, and Image J analysis of collagen fiber reconstruction ratios in tissue sections. Through comprehensive evaluation, it can be found that hydrogel dressing has analgesic, anti-inflammatory and anti-infection effects on rat wound surface, and acts on promoting wound healing, promoting the formation of new blood vessels in the damaged skin tissue area, promoting the growth of granulation tissue, and promoting the reconstruction of collagen fibers in wound tissue

Fusaric acid instigates the invasion of banana by Fusarium oxysporum f. sp. cubense TR4

CITATION: Liu, S. et al. 2020. Fusaric acid instigates the invasion of banana by Fusarium oxysporum f. sp. cubense TR4. New Phytologist, 225:913–929, doi:10.1111/nph.16193.The original publication is available at https://nph.onlinelibrary.wiley.comFusaric acid (FSA) is a phytotoxin produced by several Fusarium species and has been associated with plant disease development, although its role is still not well understood. Mutation of key genes in the FSA biosynthetic gene (FUB) cluster in Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) reduced the FSA production, and resulted in decreased disease symptoms and reduced fungal biomass in the host banana plants. When pretreated with FSA, both banana leaves and pseudostems exhibited increased sensitivity to Foc TR4 invasion. Banana embryogenic cell suspensions (ECSs) treated with FSA exhibited a lower rate of O2 uptake, loss of mitochondrial membrane potential, increased reactive oxygen species (ROS) accumulation, and greater nuclear condensation and cell death. Consistently, transcriptomic analysis of FSA-treated ECSs showed that FSA may induce plant cell death through regulating the expression of genes involved in mitochondrial functions. The results herein demonstrated that the FSA from Foc TR4 functions as a positive virulence factor and acts at the early stage of the disease development before the appearance of the fungal hyphae in the infected tissues.https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.16193Publisher's versio

Groundwater Flow Determination Using an Interval Parameter Perturbation Method

Groundwater flow simulation often inevitably involves uncertainty, which has been quantified by a host of methods including stochastic methods and statistical methods. Stochastic methods and statistical methods face great difficulties in applications. One of such difficulties is that the statistical characteristics of random variables (such as mean, variance, covariance, etc.) must be firstly obtained before the stochastic methods can be applied. The dilemma is that one is often unclear about such statistical characteristics, given the limited available data. To overcome the problems met by stochastic methods, this study provides an innovative approach in which the hydrogeological parameters and sources and sinks of groundwater flow are represented by bounded but uncertain intervals of variables called interval of uncertainty variables (IUVs) and this approach is namely the interval uncertain method (IUM). IUM requires only the maximum and minimum values of the variable. By utilizing the natural interval expansion, an interval-based parametric groundwater flow equation is established, and the solution of that equation can be found. Using a hypothetical steady-state flow case as an example, one can see that when the rate of change is less than 0.2, the relative error of this method is generally limited to less than 5%; when the rate of change is less than 0.3, the relative error of this method can be kept within 10%. This research shows that the proposed method has smaller relative errors and higher computational efficiency than the Monte Carlo methods. It is possible to use this method to analyze the uncertainties of groundwater flow when it is difficult to obtain the statistical characteristics of the hydrogeological systems. The proposed method is applicable in linear groundwater flow system. Its validity in nonlinear flow systems such as variably saturated flow or unconfined flow with considerable variation of water table will be checked in the future

Prognostic significance of the epithelial-to-mesenchymal transition markers e-cadherin, vimentin and twist in bladder cancer

ObjectiveThe goal of this study was to utilize long-term patient follow-up to determine whether epithelial-to-mesenchymal transition (EMT)-related markers can predict bladder cancer patient survival and progression of disease.Materials and MethodsThis study included 121 patients with bladder cancer. Sixty-four of these patients presented with non-muscle invasive (NMI, stage T1) bladder cancer and 57 with muscle invasive (MI, stage T2, T3). The patients were diagnosed and treated between May 1998 and July 2012. The EMT markers E-cadherin, Twist, and Vimentin were detected via immunohistochemistry. Univariate and multivariate/Cox analyses were then utilized to determine whether these EMT markers could be useful prognostic markers for predicting bladder cancer patient outcomes.ResultsAnalysis of the 121 bladder cancer patients in this study revealed that the frequency of E-cadherin expression was 59.5% (72/121), Twist was 54.5% (66/121), and Vimentin was 24.8% (30/121). Twist and Vimentin were found to have statistically significant correlations with grade, recurrence, and progression but not with stage, whereas E-cadherin was associated with stage but not with the other parameters. In the univariate analysis, grade (p = 0.02) was the only significant predictor for progression-free survival (PFS). Stage, grade, and expression of E-cadherin, Vimentin and Twist were included in the multivariate analysis of predicting PFS. In this analysis, grade (p = 0.01) and Vimentin expression (p = 0.001) were found to be significant prognostic factors in predicting PFS.ConclusionsGrade and Vimentin are potential independent indicators in predicting bladder cancer progression and survival

Sex Differences in the Association between Night Shift Work and the Risk of Cancers: A Meta-Analysis of 57 Articles

Objectives. To identify the association between night shift work and the risk of various cancers with a comprehensive perspective and to explore sex differences in this association. Methods. We searched PubMed, Embase, and Web of Science for studies on the effect of night shift work on cancer, including case-control, cohort, and nested case-control studies. We computed risk estimates with 95% confidence intervals (CIs) in a random or fixed effects model and quantified heterogeneity using the I2 statistic. Subgroup, metaregression, and sensitivity analyses were performed to explore potential sources of heterogeneity. Contour-enhanced funnel plots and the trim and fill method were used together to analyze bias. Linear dose–response analysis was used to quantitatively estimate the accumulative effect of night shift work on the risk of cancer. Results. Fifty-eight studies were eligible for our meta-analysis, including 5,143,838 participants. In the random effects model, the pooled odds ratio (OR) of cancers was 1.15 (95% CI = 1.08–1.22, P<0.001; I2=76.2%). Night shift work increased the cancer risk in both men (OR = 1.14, 95% CI = 1.05–1.25, P=0.003) and women (OR = 1.12, 95% CI = 1.04–1.20, P=0.002). Subgroup analyses showed that night shift work positively increased the risk of breast (OR = 1.22, 95% CI = 1.08–1.38), prostate (OR = 1.26, 95% CI = 1.05–1.52), and digestive system (OR = 1.15, 95% CI = 1.01–1.32) cancers. For every 5 years of night shift work, the cancer risk increased by 3.2% (OR = 1.032, 95% CI = 1.013–1.051). Conclusion. This is the first meta-analysis identifying the positive association between night shift work and the risk of cancer and verifying that there is no sex difference in the effect of night shift work on cancer risk. Cancer risk increases with cumulative years of night shift work

Phosphorus Dynamics in Long-Term Flooded, Drained, and Reflooded Soils

In flooded areas, soils are often exposed to standing water and subsequent drainage, thus over fertilization can release excess phosphorus (P) into surface water and groundwater. To investigate P release and transformation processes in flooded alkaline soils, wheat-growing soil and vegetable-growing soil were selected. We flooded-drained-reflooded two soils for 35 d, then drained the soils, and 10 d later reflooded the soils for 17 d. Dissolved reactive phosphorus (DRP), soil inorganic P fractions, Olsen P, pH, and Eh in floodwater and pore water were analyzed. The wheat-growing soil had significantly higher floodwater DRP concentrations than vegetable-growing soil, and floodwater DRP in both soils decreased with the number of flooding days. During the reflooding period, DRP in overlying floodwater from both soils was less than 0.87 mg/L, which was 3–25 times less than that during the flooding period. Regardless of flooding or reflooding, pore water DRP decreased with flooding days. The highest concentration of pore water DRP observed at a 5-cm depth. Under the effect of fertilizing and flooding, the risk of vertical P movement in 10–50 cm was enhanced. P diffusion occurred from the top to the bottom of the soils. After flooding, Al-P increased in both soils, and Fe-P, O-P, Ca2-P decreased, while Fe-P, Al-P, and O-P increased after reflooding, When Olsen P in the vegetable-growing soil exceeded 180.7 mg/kg and Olsen P in the wheat-growing soil exceeded 40.8 mg/kg, the concentration of DRP in pore water increased significantly. Our results showed that changes in floodwater and pore water DRP concentrations, soil inorganic P fractions, and Olsen P are significantly affected by fertilizing and flooding; therefore, careful fertilizer management should be employed on flooded soils to avoid excess P loss

Numerical simulation of the water budget interval for unsteady two-dimensional confined flow

Objective Groundwater numerical models often have uncertainties due to the complexity of the hydrogeological conditions and the economic and time constraints in collecting a sufficiently large dataset as inputs for conducting modelling exercises. In the past 50 years, stochastic methods have been one of the main methods of uncertainty analysis. The interval uncertainty is different from the stochastic uncertainty, and it considers the hydrogeological parameters as the intervals (ranges) without considering their stochastic properties. Methods From the perspective of interval uncertainty, a numerical simulation method based on first-order perturbation expansion was proposed for simulating unsteady two-dimensional confined flow with known hydrogeological parameters as intervals in this paper.The proposed method is implemented based on GFModel, a three-dimensional (3D) numerical simulation platform for groundwater flow and pollutant migration. Results The analysis shows that the relative error can be controlled within 10% when the parameter change rate is less than 0.1. The computational efficiency of the proposed method is obviously higher than that of the continuous sampling method with equal spacing. Conclusion This method allows the interval of the head or water budget to be calculated without the requirement for detailed statistical information (which is usually unavailable in advance) if the intervals of hydrogeological parameters are known.It provides a theoretical basis for decisions on the use and protection of groundwater resources

Mixed-well model of the relation between drawdown and water inflow in a pumping well with variable-diameter

Objective Pumping tests are an important method for determining the hydrogeological parameters of aquifers and evaluating groundwater resources. For the analysis of single-well pumping tests, existing models assume that the well diameter remains constant with depth and that the aquifer is either unconfined or confined. They do not consider situations where the well diameter varies or when multiple aquifers are encountered. Methods In this study, we developed a steady-state mixed-well flow model to account for a pumping well with a variable diameter that penetrates multiple aquifers with the assumption of horizontal flow within the aquifers. The analytical solutions for the relation between pumping discharge and drawdown were derived. This study explores the methodology of using single-well steady-state pumping tests with variable-diameter wells to obtain aquifer parameters. Additionally, accurate solutions for the equivalent radius of a confined aquifer segment and alternative radius calculation formulas are proposed. Results The mixed-well flow model was applied to analyze pumping tests near the Zihe River. Based on the data from three stepped pumping tests, a parabolic relationship between pumping discharge Qw and drawdown sw was established.The model predicted a pumping discharge of 4 093.8 m3/d when the maximum drawdown sw was 25 m. The hydraulic conductivity of the unconfined aquifer was estimated to be 1.88 m/d, and the hydraulic conductivity of the confined aquifer was estimated as 0.43 m/d, with a relative error of less than 5%. Conclusion The mixed-well model serves as a theoretical foundation for predicting water yield from pumping wells in multiple aquifers system. However, it is important to acknowledge that the model's applicability is constrained by certain assumptions. In situations where rivers fully penetrate aquifers, there is a possibility of overestimating the hydraulic conductivity. On the other hand, when rivers only partially penetrate aquifers, the analytical solution may underestimate the hydraulic conductivity

Polymer electrolyte glue : a universal interfacial modification strategy for all-solid-state Li batteries

In recent years solid Li+ conductors with competitive ionic conductivity to those of liquid electrolytes have been reported. However, the incorporation of highly conductive solid electrolytes into the lithium-ion batteries is still very challenging mainly due to the high resistance existing at the solid-solid interfaces throughout the battery structure. Here, we demonstrated a universal interfacial modification strategy through coating a curable polymer-based glue electrolyte between the electrolyte and electrodes, aiming to address the poor solid-solid contact and thus decrease high interfacial resistance. The liquid glue exhibits both great wettability as well as chemical/electrochemical stability to most of the electrodes, and it can be easily solidified into a polymer electrolyte layer through a "post-curing" treatment. As a result, symmetric Li batteries with the glue modification exhibit much smaller impedance and enhanced stability upon plating/stripping cycles compared to the batteries without glue modification. The all-solid-state Li-S batteries with glue modification show significantly enhanced performances. The strategy of developing glue electrolytes to improve the electrode-electrolyte interface contact provides an alternative option for improving many other solid-state batteries.Accepted versio