Discussion on the Effect of Emergency Tracheal Intubation on Cardiopulmonary Resuscitation by Emergency Medical Staff

Objective: To study the positive effect of emergency tracheal intubation on cardiopulmonary resuscitation. Methods: 90 patients with cardiopulmonary arrest were randomly selected from the emergency department of our hospital from November 2017 to November 2019, and were randomly divided into the control group and the experimental group (n=45). The control group was given routine anesthesia combined with cardiopulmonary resuscitation, while the experimental group was given emergency tracheal intubation combined with cardiopulmonary resuscitation. The effect of cardiopulmonary resuscitation and operation time of the two groups were observed and discussed, and the results were recorded. Results: Under different intervention measures, the time from visiting a doctor to tracheal intubation in the experimental group was obviously shorter than that in the control group (P < 0.05). There is no significant difference in the time of intubation between the two groups (P > 0.05). In addition, the success probability of cardiopulmonary resuscitation and the discharge survival rate in the experimental group were higher than those in the control group (P < 0.05). The above-mentioned results with p < 0.05 indicated the statistically significant differences. Conclusion: In clinical practice, emergency tracheal intubation for patients with cardiopulmonary arrest by emergency medical staff can bring a higher success probability of cardiopulmonary resuscitation, buy valuable time for rescue operation, and obviously improve the prognosis of patients. Therefore, it is worthy of wider promotion and application

METS-Based Cataloging Toolkit for Digital Library Management System

This toolkit is designed for the Digital Library Management System of Tsinghua University (TH-DLMS). The aim of TH-DLMS is to build up a platform to preserve various kinds of digitalized resources, manage distributed repositories and provide kinds of service for research and education. This toolkit fulfills the cataloging and preservation functions of TH-DLMS. METS (Metadata Encoding and T ransmission Standard) encoded documents are used as the final storage format of metadata, including descriptive metadata, structural metadata and administrative metadata, and submitted to a management system based on Fedora (Flexible Extensible Digital Object and Repository Architecture)

A hybrid ensemble forecasting model of passenger flow based on improved variational mode decomposition and boosting

An accurate passenger flow forecast can provide key information for intelligent transportation and smart cities, and help promote the development of smart cities. In this paper, a mixed passenger flow forecasting model based on the golden jackal optimization algorithm (GJO), variational mode decomposition (VMD) and boosting algorithm was proposed. First, the data characteristics of the original passenger flow sequence were extended. Second, an improved variational modal decomposition method based on the Sobol sequence improved GJO algorithm was proposed. Next, according to the sample entropy of each intrinsic mode function (IMF), IMF with similar complexity is combined into a new subsequence. Finally, according to the determination rules of the sub-sequence prediction model, the boosting modeling and prediction of different sub-sequences were carried out, and the final passenger flow prediction result was obtained. Based on the experimental results of three scenic spots, the mean absolute percentage error (MAPE) of the mixed set model is 0.0797, 0.0424 and 0.0849, respectively. The fitting degree reached 95.33%, 95.63% and 95.97% simultaneously. The results show that the hybrid model proposed in this study has high prediction accuracy and can provide reliable information sources for relevant departments, scenic spot managers and tourists

Improving interface bonding of double-skinned CFST columns

It has been demonstrated that high-strength concrete (HSC) is able to improve the strength-to-weight ratio of reinforced concrete columns and maximise the usable areas of tall buildings. However, closely spaced transverse reinforcement needs to be installed to provide stronger confinement for averting brittle failure of HSC. To resolve the problem, double-skinned concrete-filled-steel-tubular (CFST) columns have been advocated, which eliminates the steel congestion problem for better concrete placing and reduces the concrete arching action thus providing a more uniform confining pressure. Despite these advantages, a major shortcoming of double-skinned CFST columns is that imperfect interface bonding occurs in the elastic stage that reduces elastic strength and stiffness. Thus, the authors proposed to adopt external confinement to restrict the lateral dilation of the outer tube of double-skinned CFST columns. To verify the effectiveness of the proposed external rings, a total of 20 double-skinned normal- and high-strength CFST columns were tested. From the test results, it was observed that the stiffness, axial load-carrying capacity and ductility of ringconfined double-skinned CFST columns were significantly higher than the unconfined columns

A Hierarchical Security Framework for Defending Against Sophisticated Attacks on Wireless Sensor Networks in Smart Cities

In smart cities, wireless sensor networks (WSNs) act as a type of core infrastructure that collects data from the city to implement smart services. The security of WSNs is one of the key issues of smart cities. In resource-restrained WSNs, dynamic ongoing or unknown attacks usually steer clear of isolated defense components. Therefore, to resolve this problem, we propose a hierarchical framework based on chance discovery and usage control (UCON) technologies to improve the security of WSNs while still taking the low-complexity and high security requirements of WSNs into account. The features of continuous decision and dynamic attributes in UCON can address ongoing attacks using advanced persistent threat detection. In addition, we use a dynamic adaptive chance discovery mechanism to detect unknown attacks. To design and implement a system using the mechanism described above, a unified framework is proposed in which low-level attack detection with simple rules is performed in sensors, and high-level attack detection with complex rules is performed in sinks and at the base station. Moreover, software-defined networking and network function virtualization technologies are used to perform attack mitigation when either low-level or high-level attacks are detected. An experiment was performed to acquire an attack data set for evaluation. Then, a simulation was created to evaluate the resource consumption and attack detection rate. The results demonstrate the feasibility and efficiency of the proposed scheme

Stable Reference Gene Selection for RT-qPCR Analysis in Nonviruliferous and Viruliferous \u3cem\u3eFrankliniella occidentalis\u3c/em\u3e

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable technique for measuring and evaluating gene expression during variable biological processes. To facilitate gene expression studies, normalization of genes of interest relative to stable reference genes is crucial. The western flower thrips Frankliniella occidentalis (Pergande) (Thysanoptera: Thripidae), the main vector of tomato spotted wilt virus (TSWV), is a destructive invasive species. In this study, the expression profiles of 11 candidate reference genes from nonviruliferous and viruliferous F. occidentalis were investigated. Five distinct algorithms, geNorm, NormFinder, BestKeeper, the ΔCt method, and RefFinder, were used to determine the performance of these genes. geNorm, NormFinder, BestKeeper, and RefFinder identified heat shock protein 70 (HSP70), heat shock protein 60 (HSP60), elongation factor 1 α, and ribosomal protein l32 (RPL32) as the most stable reference genes, and the ΔCt method identified HSP60, HSP70, RPL32, and heat shock protein 90 as the most stable reference genes. Additionally, two reference genes were sufficient for reliable normalization in nonviruliferous and viruliferous F. occidentalis. This work provides a foundation for investigating the molecular mechanisms of TSWV and F. occidentalis interactions

Preliminary investigation of an SOI-based arrayed waveguide grating demodulation integration microsystem

An arrayed waveguide grating (AWG) demodulation integration microsystem is investigated in this study. The system consists of a C-band on-chip LED, a 2 × 2 silicon nanowire-based coupler, a fiber Bragg grating (FBG) array, a 1 × 8 AWG, and a photoelectric detector array. The coupler and AWG are made from silicon-on-insulator wafers using electron beam exposure and response-coupled plasma technology. Experimental results show that the excess loss in the MMI coupler with a footprint of 6 × 100 μm(2) is 0.5423 dB. The 1 × 8 AWG with a footprint of 267 × 381 μm(2) and a waveguide width of 0.4 μm exhibits a central channel loss of −3.18 dB, insertion loss non-uniformity of −1.34 dB, and crosstalk level of −23.1 dB. The entire system is preliminarily tested. Wavelength measurement precision is observed to reach 0.001 nm. The wavelength sensitivity of each FBG is between 0.04 and 0.06 nm/dB

326 The anti-TIGIT antibody M6223 induces significant anti-tumor efficacy and immune response via multiple mechanisms of action

BackgroundM6223 is a fully human antagonistic anti-T cell immunoreceptor with immunoglobulin and ITIM domains (TIGIT) antibody in IgG1 format with Fc-mediated effector function.MethodsThe ability of M6223 to block the interaction of TIGIT with its ligands, CD155 and CD112, and the interaction of TIGIT with CD226 was determined by a flow cytometry-based binding assay. The anti-tumor efficacy, immune profile, and effector function of M6223 were investigated in syngeneic tumor models in huTIGIT knock-in mice. M6223 was either formatted with an effector competent mouse IgG2c constant region (M6223-muIgG2c) or formatted with effector null mouse IgG1-D256A constant region (M6223-muIgG1) as two versions of chimeric antibodies for the in vivo studies.ResultsM6223 dose-dependently blocked the binding of TIGIT to its ligands, including CD155 and CD112, thereby inhibiting a TIGIT-mediated immunosuppressive pathway. In addition, M6223 interrupted the interaction of TIGIT with the costimulatory receptor CD226. By blocking the interactions, the chimeric protein M6223-muIgG2c showed anti-tumor efficacy in multiple tumor models, including an MC38 tumor model (figure 1), and generated tumor antigen-specific long-term protective immunity in immunocompetent huTIGIT knock-in mice. M6223 monotherapy dose-dependently elevated the ratio of CD8+ cytotoxic T cells to regulatory T cells and the ratio of CD226 to TIGIT expression in immune cells in the tumor microenvironment. We also found that M6223 selectively depleted suppressive and exhausted TIGIT+ immune cell subsets and the anti-tumor activity of effector null M6223-muIgG1 was significantly lost (p<0.0001), suggesting that Fc-mediated effector function contributes to M6223 anti-tumor activity. Antibody depletion studies demonstrated that CD8+ T cells and natural killer cells contributed to the anti-tumor activity of M6223 in a complementary manner.Abstract 326 Figure 1M6223-muIgG2c displayed dose-dependent anti-tumor efficacy. M6223-muIgG2c displayed dose-dependent anti-tumor efficacy in an MC38 tumor model in hTIGIT knock-in mice.ConclusionsGiven that TIGIT blockade can inhibit an immunosuppressive pathway as well as remove the suppression on a costimulatory pathway, M6223 has the potential to induce an anti-tumor immune response by three complementary mechanisms: direct blockade of the TIGIT pathway, stimulation of CD226 dimerization/activation, and depletion of TIGIT+ immune subsets by Fc-mediated effector function. Our data demonstrate that these complementary mechanisms orchestrate the anti-tumor activity of M6223. A Phase I, first-in-human clinical trial (NCT04457778) is underway to determine the safety, tolerability, maximum tolerated dose and recommended dose for expansion of M6223 as a single agent (Part 1A) and in combination with bintrafusp alfa (Part 1B) in patients with metastatic or locally advanced solid unresectable tumors.Ethics ApprovalAll animal experiments were performed in accordance with EMD Serono Research & Development Institute, Inc., Billerica, MA, USA, an affiliate of Merck KGaA (protocol 17-008, 20-005) and Wuxi AppTec Animal Care and Use Committee (IACUC) guidelines

15-Lipoxygenase-1 Is Involved in the Effects of Atorvastatin on Endothelial Dysfunction

Statins exert pleiotropic effects on endothelial cells in addition to lowering cholesterol. 15-Lipoxygenase-1 (ALOX15) has been implicated in vascular inflammation and disease. The relationship between atorvastatin and ALOX15 was investigated using a rat carotid artery balloon-injury model. Hematoxylin and eosin (HE) staining showed that ALOX15 overexpression increased the thickness of the intima-media (IMT). Immunohistochemistry and western blotting showed that atorvastatin increased the expression of cellular adhesion molecules (CAMs) but decreased the expression of endothelial nitric oxide synthase (eNOS); these effects of atorvastatin were blocked by ALOX15 overexpression. In human umbilical venous endothelial cells (HUVECs), silencing of ALOX15 enhanced the effects of atorvastatin on endothelial function. Expression levels of CAMs and Akt/eNOS/NO under oxidized low-density lipoprotein (ox-LDL) stimulation were modulated by ALOX15 inhibitor and ALOX15 small interfering RNA (siRNA). Atorvastatin abolished the activation of nuclear factor-kappa B (NF-κB) induced by ox-LDL. Exposure to ox-LDL induced upregulation of ALOX15 in HUVECs, but this effect was partially abolished by atorvastatin or the NF-κB inhibitor pyrrolidine dithiocarbamate (PDTC). These results demonstrate that regulation of ALOX15 expression might be involved in the effects of atorvastatin on endothelial dysfunction