Development of caprine preantral follicles after orthotopic autotransplantation of ovarian tissue

Objective: The aim of this study was to evaluate the follicle morphology, density, development and hormone production after orthotopic autransplantation of fresh or vitrified goat ovarian tissue. Methods: Fresh and vitrified ovarian cortex was orthotopically autotransplanted for six months in two and three adults bilaterally ovariectomized goats, respectively. The animals were monitored during 196 days and blood samples collected. Results: It was observed that the percentage of morphologically normal preantral follicles (MNPF) after grafting of fresh ovarian tissue was similar to control. The follicular density in the fresh graft reduced significantly when compared to fresh control. unfortunately, after transplantation of vitrified tissue it was not possible to identified any follicles after recovery. Furthermore, the proportion of developing follicles was higher (P < 0.05) in the fresh auto-grafts than in control fragments. Moreover, progesterone plasma levels increased significantly from day 179 to day 195 of transplantation. Conclusion: In conclusion, orthotopic transplantation of fresh ovarian tissue was able to keep healthy the preantral follicles, as well as the restoration of goat endocrine function

Interaction of the hippo signaling pathway during bovine follicle activation and growth in vitro

info:eu-repo/semantics/publishe

Control of in vitro follicle activation by inhibition of YAP/TAZ activity in mouse ovaries

info:eu-repo/semantics/publishe

Regulation of follicular activation signaling pathways by in vitro inhibition of YAP/TAZ activity in mouse ovaries

Abstract The Hippo pathway plays a crucial role in the regulation of follicular activation, which constitutes the first step of the folliculogenesis process. Disruption of this pathway occurs in several non-physiological contexts, after fragmentation for ovarian tissue cryopreservation procedures or chemotherapy exposure, leading to massive follicular growth and depletion. This study aimed to investigate the effect of controlling the Hippo pathway using verteporfin (VERT) during in vitro ovarian culture and to evaluate its potential preventive effects on chemotherapy-induced follicle activation using a mouse model. After exposure of cut ovaries to different concentrations of VERT for 3 h, a dose-dependent effect of VERT was observed that reached significant inhibition of YAP activity at 3 µmol/L. To assess the potential effect of controlling chemotherapy-induced Hippo pathway disruption, whole mouse ovaries were exposed to VERT alone or as a co-treatment with 4-hydroperoxycylophosphamide (4HC). VERT co-treatment prevented chemotherapy-induced YAP activation but had a limited impact on downstream effector gene, Ccn2. Surprisingly, VERT co-treatment also prevented mTOR and survival signaling pathway alterations following chemotherapy exposure. These results suggest an interaction between the two main signaling pathways regulating follicle activation and a protective effect of VERT on 4HC-induced DNA damage

Development of caprine preantral follicles after orthotopic autotransplantation of ovarian tissue

Objective: The aim of this study was to evaluate the follicle morphology, density, development and hormone production after orthotopic autransplantation of fresh or vitrified goat ovarian tissue. Methods: Fresh and vitrified ovarian cortex was orthotopically autotransplanted for six months in two and three adults bilaterally ovariectomized goats, respectively. The animals were monitored during 196 days and blood samples collected. Results: It was observed that the percentage of morphologically normal preantral follicles (MNPF) after grafting of fresh ovarian tissue was similar to control. The follicular density in the fresh graft reduced significantly when compared to fresh control. unfortunately, after transplantation of vitrified tissue it was not possible to identified any follicles after recovery. Furthermore, the proportion of developing follicles was higher (P < 0.05) in the fresh auto-grafts than in control fragments. Moreover, progesterone plasma levels increased significantly from day 179 to day 195 of transplantation. Conclusion: In conclusion, orthotopic transplantation of fresh ovarian tissue was able to keep healthy the preantral follicles, as well as the restoration of goat endocrine function

Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis.

The present study evaluated the effect of supplementing in vitro culture medium with J. insularis compared to FSH on isolated secondary follicles and in vitro maturation of oocytes from those follicles. Secondary follicles were isolated from sheep ovaries and individually cultured for 18 days in α-MEM+ (Control), α-MEM+ supplemented with 100 ng/mL recombinant bovine follicle stimulating hormone (FSH) or with 0.3, 1.25, or 2.5 mg/mL of J. insularis extract (JI0.3, JI1.25, and JI2.5, respectively). Culture medium collected every 2 days was used to measure ROS levels. At the end of the culture period, cumulus oocytes complex (COCs) were collected and matured in vitro. Follicular walls were used for mRNA quantitation. JI0.3 led to a higher (P < 0.05) percentages of intact follicles than other groups after 18 days of culture. While follicular diameter remained unchanged from Day 6 onwards with JI0.3 and FSH, percentages of antral cavity formation were higher (P < 0.05) with JI0.3 at Day 6 than in all other treatments. No differences were observed between controls and treatment groups regarding ROS levels and mRNA expression of genes. Viability of resulting oocytes was higher (P < 0.05) in JI0.3 compared to FSH. Interestingly, in control experiment, supplementation of maturation medium with JI0.3 led to higher (P < 0.05) percentages of metaphase II compared to controls. Although more validations will be needed, it seems that this natural extract could be used as a cheap and easily available alternative to commercial FSH