Immunoglobulins in Human Seminal Plasma

Single radial immunodiffusion has been used to evaluate immunoglobulins and secretory piece (SP) in human seminal plasma. Samples were collected from 90 healthy volunteers, 202 subjects submitted to vasectomy for contraceptive purposes, tested at various intervals after surgery, and 725 patients grouped according to selected andrological disorders. Results may be summarized as follows. In normal subjects IgG and IgA were constantly present (mean values +/- SD: 8.14 +/- 2.82 mg/dl; 1.91 +/- 1.03 mg/dl, respectively) while IgM were detected in trace amounts (from 0.7 to 3.3 mg/dl) in 10% of subjects, and negligible or absent in the remaining subjects. In vasectomized subjects IgG and IgA showed a significant increase only in the first 3 months after vasectomy, probably due to surgery. In andrological patients Ig showed an increase in cases with antisperm antibodies (A b) and in those with infections of genital tract and positive semen culture. On the basis of these findings and the secretory piece assay data the importance appears to be stressed of the local immunocompetent system at least in some andrological diseases

Aerobic Capacity Independently Predicts Central Augmentation Index Among Apparently Healthy Adults

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Four Months of Kettlebell Training Improves Muscle Strength and Functional Outcomes in Older Adults

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Health professional educators’ experiences of interprofessional socialisation within higher education: An interpretative phenomenological study

This interpretative phenomenological study explored health professional educators’ understanding and experiences of inter-professional socialisation within higher education in Perth, WA. The analysis of one-to-one interviews comprised of 26 HPEs’ from various health related disciplines across 5 universities within WA. Qualitative content analysis led to the development of five themes. A newly developed Health Educators Inter-Professional Socialisation framework is proposed along with socialisation strategies that could positively influence IP collaboration between educators within higher education

Sex Differences in Augmentation Index in Response to Acute Dynamic Exercise

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Cell-laden hydrogel as a clinical-relevant 3D model for analyzing neuroblastoma growth, immunophenotype, and susceptibility to therapies

High risk Neuroblastoma (NB) includes aggressive, metastatic solid tumors of childhood. The survival rate improved only modestly, despite the use of combination therapies including novel immunotherapies based on the antibody mediated targeting of tumor-associated surface ligands. Treatment failures may be due to the lack of adequate in vitro models for studying, in a given patient, the efficacy of potential therapeutics, including those aimed to enhance anti-tumor immune responses. We here propose a 3D alginate-based hydrogel as extracellular microenvironment to evaluate the effects of the three-dimensionality on biological and immunological properties of NB cells. NB cell lines grown within the 3D alginate spheres presented spheroid morphology, optimal survival, and proliferation capabilities, and a reduced sensitivity to the cytotoxic effect of imatinib mesylate. 3D cultured NB cells were also evaluated for the constitutive and IFN-y-induced expression of surface molecules capable of tuning the anti-tumor activity of NK cells including immune checkpoint ligands. In particular, IFN-y induced de novo expression of high amounts of HLA-I molecules, which protected NB cells from the attack mediated by KIR/KIR-L matched NK cells. Moreover, in the 3D alginate spheres, the cytokine increased the expression of the immune checkpoint ligands PD-Ls and B7-H3 while virtually abrogating that of PVR, a ligand of DNAM-1 activating receptor, whose expression correlates with high susceptibility to NK-mediated killing. Our 3D model highlighted molecular features that more closely resemble the immunophenotypic variants occurring in vivo and not fully appreciated in classical 2D culture conditions.Thus, based on our results, 3D alginate-based hydrogels might represent a clinical-relevant cell culture platform where to test the efficacy of personalized therapeutic approaches aimed to optimize the current and innovative immune based therapies in a very systematic and reliable way