Characterization of an optically readout gaseous time projection chamber with a 55^{55}Fe source

The nature of dark matter (DM) is currently one of the most intriguing questions of fundamental physics. Even if the question of dark matter finds its roots in astrophysics and cosmology, it is also actively searched for in particle physics experiments, at colliders as well as in dark matter detection experiments, which aim at discovering dark matter particles. The main experimental challenge comes of course from the elusive nature of DM. One of the most important observables connecting dark matter, cosmology and particle physics is the cosmological density of DM, which is called in this context the relic density. In the last years, several experiments were performed to detect DM particles and some of these experiments use a direct directional detection of WIMP particles. This thesis is an experimental thesis about the development of a new type of detector, called CYGNO. This detector is composed by a Time Projection Chamber filled with a gas mixture at atmospheric pressure. A triple GEM-system is used to produce the light in the detector. By using an optical readout the light is collected

Complete resolution of hepatic aspergillosis after non-myeloablative hematopoietic stem cell transplantation in a patient with acute myeloid leukemia

Fungal infections due to Aspergillus are a frequent cause of transplant-related mortality. For this reason, leukemic patients with severe fungal infection are usually excluded from conventional allotransplantation. Recently, some authors suggested a role for non-myeloablative hematopoietic stem cell transplantation (HSCT) in this subset of patients. We used this therapeutic approach in a patient with high-risk acute myeloid leukemia in second complete remission (CR) with pre-existing hepatic aspergillosis refractory to conventional anti-fungal therapy. A complete regression of hepatic lesions was observed after 3 months from allogeneic stem cell transplantation. Our work confirms previous reports suggesting that non-myeloablative HSCT is effective in patients not eligible for conventional transplantation because of invasive aspergillosis

Lysosomal Acid Lipase as a Molecular Target of the Very Low Carbohydrate Ketogenic Diet in Morbidly Obese Patients: The Potential Effects on Liver Steatosis and Cardiovascular Risk Factors

A very low carbohydrate ketogenic diet (VLCKD) is an emerging technique to induce a significant, well-tolerated, and rapid loss of body weight in morbidly obese patients. The low activity of lysosomal acid lipase (LAL) could be involved in the pathogenesis of non-alcoholic fatty liver disease (NAFLD), which is a common feature in morbidly obese patients. Fifty-two obese patients suitable for a bariatric surgery intervention underwent a 25-day-long VLCKD. The biochemical markers of glucose and lipid metabolism, and flow-mediated dilation (FMD) of the brachial artery were measured before and after VLCKD. LAL activity was measured using the dried blood spot technique in 20 obese patients and in a control group of 20 healthy, normal-weight subjects. After VLCKD, we observed a significant reduction in body mass index, fasting glucose, insulinemia, and lipid profile parameters. No significant variation in FMD was observed. The number of patients with severe liver steatosis significantly decreased. LAL activity significantly increased, although the levels were not significantly different as compared to the control group. In conclusion, VLCKD induces the activity of LAL in morbidly obese subjects and reduces the secretion of all circulating lipoproteins. These effects could be attributed to the peculiar composition of the diet, which is particularly poor in carbohydrates and relatively rich in proteins

Efficacy of anti-CD20 monoclonal antibodies (Mabthera) in patients with progressed hairy cell leukemia

BACKGROUND AND OBJECTIVES: Recently, a chimeric monoclonal antibody (MoAb) directed against the CD20 antigen (rituximab) has been successfully introduced in the treatment of several CD20-positive B-cell neoplasias and particularly of follicular lymphomas. Based on these premises we evaluated the efficacy and the toxicity of chimeric anti-CD20 monoclonal antibody (MoAb) in relapsed/progressed hairy cell leukemia (HCL).DESIGN AND METHODS: Ten patients with relapsed/progressed HCL entered the study. Eight patients were males and two females with a median age of 55 years (range 41-78) and all of them had been previously treated with 2-chlorodeoxyadenosine and/or deoxycoformycin and a-interferon. Two out of 10 patients were anemic (Hb < 10 g/dL), 4 thrombocytopenic (Plt < 100 x 10(9)/L), 3 had fewer than 1.0 x 10(9)/L neutrophils and 3 had circulating hairy cells (HC). All patients received 375 mg/m2 i.v. of anti-CD20 MoAb once a week for 4 doses.RESULTS: All patients were evaluable for response, one patient showing a complete remission and 4 a partial response. Adverse reactions, such as fever, chills, bone pain, hypotension and thrombocytopenia, were transient and mild (grade 1-2) and occurred only during the first course of treatment. One month after the last infusion, patients who had had anemia, neutropenia or thrombocytopenia, recovered normal peripheral blood values. Circulating HC also disappeared within one month. Immunostained bone marrow biopsies were checked 1, 3 and 6 months after the end of therapy and in 5 out of 10 patients a >50% reduction of bone marrow HC infiltration was recorded.INTERPRETATION AND CONCLUSIONS: On the basis of these preliminary results observed in 10 patients with progressed HCL, it appears that treatment with anti-CD20 MoAb is safe and effective in at least 50% of patients, particularly in those with a less evident bone marrow infiltration (50%) and in those previously splenectomized

A Pilot Interaction Analysis of Gut Microbiota and Peripheral Markers of Aging in Severe Psychiatric Disorders: A Role for Lachnoclostridium?

Excessive predominance of pathological species in the gut microbiota could increase the production of inflammatory mediators at the gut level and, via modification of the gut–blood barrier, at the systemic level. This pro-inflammatory state could, in turn, increase biological aging that is generally proxied by telomere shortening. In this study, we present findings from a secondary interaction analysis of gut microbiota, aging, and inflammatory marker data from a cohort of patients with different diagnoses of severe mental disorders. We analyzed 15 controls, 35 patients with schizophrenia (SCZ), and 31 patients with major depressive disorder (MDD) recruited among those attending a community mental health center (50 males and 31 females, mean and median age 46.8 and 46.3 years, respectively). We performed 16S rRNA sequencing as well as measurement of telomere length via quantitative fluorescence in situ hybridization and high-sensitivity C-reactive protein. We applied statistical modeling with logistic regression to test for interaction between gut microbiota and these markers. Our results showed statistically significant interactions between telomere length and gut microbiota pointing to the genus Lachnostridium, which remained significantly associated with a reduced likelihood of MDD even after adjustment for a series of covariates. Although exploratory, these findings show that specific gut microbiota signatures overexpressing Lachnoclostridium and interacting with biological aging could modulate the liability for MDD

HRMOS White Paper: Science Motivation

International audienceThe High-Resolution Multi-Object Spectrograph (HRMOS) is a facility instrument that we plan to propose for the Very Large Telescope (VLT) of the European Southern Observatory (ESO), following the initial presentation at the VLT 2030 workshop held at ESO in June 2019. HRMOS provides a combination of capabilities that are essential to carry out breakthrough science across a broad range of active research areas from stellar astrophysics and exoplanet studies to Galactic and Local Group archaeology. HRMOS fills a gap in capabilities amongst the landscape of future instrumentation planned for the next decade. The key characteristics of HRMOS will be high spectral resolution (R = 60000 - 80000) combined with multi-object (20-100) capabilities and long term stability that will provide excellent radial velocity precision and accuracy (10m/s). Initial designs predict that a SNR~100 will be achievable in about one hour for a star with mag(AB) = 15, while with the same exposure time a SNR~ 30 will be reached for a star with mag(AB) = 17. The combination of high resolution and multiplexing with wavelength coverage extending to relatively blue wavelengths (down to 380 nm), makes HRMOS a spectrograph that will push the boundaries of our knowledge and that is envisioned as a workhorse instrument in the future. The science cases presented in this White Paper include topics and ideas developed by the Core Science Team with the contributions from the astronomical community, also through the wide participation in the first HRMOS Workshop (https://indico.ict.inaf.it/event/1547/) that took place in Firenze (Italy) in October 2021

HRMOS White Paper: Science Motivation

The High-Resolution Multi-Object Spectrograph (HRMOS) is a facility instrument that we plan to propose for the Very Large Telescope (VLT) of the European Southern Observatory (ESO), following the initial presentation at the VLT 2030 workshop held at ESO in June 2019. HRMOS provides a combination of capabilities that are essential to carry out breakthrough science across a broad range of active research areas from stellar astrophysics and exoplanet studies to Galactic and Local Group archaeology. HRMOS fills a gap in capabilities amongst the landscape of future instrumentation planned for the next decade. The key characteristics of HRMOS will be high spectral resolution (R = 60000 - 80000) combined with multi-object (20-100) capabilities and long term stability that will provide excellent radial velocity precision and accuracy (10m/s). Initial designs predict that a SNR~100 will be achievable in about one hour for a star with mag(AB) = 15, while with the same exposure time a SNR~ 30 will be reached for a star with mag(AB) = 17. The combination of high resolution and multiplexing with wavelength coverage extending to relatively blue wavelengths (down to 380 nm), makes HRMOS a spectrograph that will push the boundaries of our knowledge and that is envisioned as a workhorse instrument in the future. The science cases presented in this White Paper include topics and ideas developed by the Core Science Team with the contributions from the astronomical community, also through the wide participation in the first HRMOS Workshop (https://indico.ict.inaf.it/event/1547/) that took place in Firenze (Italy) in October 2021

A 50 l

The nature of dark matter is still unknown and an experimental program to look for dark matter particles in our Galaxy should extend its sensitivity to light particles in the GeV mass range and exploit the directional information of the DM particle motion (Vahsen et al. in CYGNUS: feasibility of a nuclear recoil observatory with directional sensitivity to dark matter and neutrinos, arXiv:2008.1258