Plot showing Bulked Segregant Analysis results for the CiC3248-06 (A) and CiC6243-03 (B) markers, distinguishing between susceptible and resistant genotypes and bulks.

<p>Plot showing Bulked Segregant Analysis results for the CiC3248-06 (A) and CiC6243-03 (B) markers, distinguishing between susceptible and resistant genotypes and bulks.</p

Order and location of markers and <i>ABSr</i> locus.

<p>(A) De novo genetic mapping (cM) of markers and the <i>ABSr</i> locus on chromosome III relative to the centromere by half-tetrad analysis, (B) relation between genetic and physical location in the ‘Clementine’ reference genome (<a href="http://www.phytozome.net/clementine" target="_blank">www.phytozome.net/clementine</a>), and (C) representation of the markers on the first axis of the multiple correspondence analysis.</p

Leaves of resistant genotype ‘Willowleaf’ mandarin (A) and susceptible genotype ‘Fortune’ mandarin (B) showing ABS symptoms 48h after inoculation with a suspension of 10⁵ conidia·ml^-1.

<p>Leaves of resistant genotype ‘Willowleaf’ mandarin (A) and susceptible genotype ‘Fortune’ mandarin (B) showing ABS symptoms 48h after inoculation with a suspension of 10⁵ conidia·ml^-1.</p

Pattern of F statistic from ANOVA along chromosome III

<div><p><b>(the linkage group map under the F value graph is taken from the ‘Clementine’ genetic map [71]</b>. </p> <p>The blue line indicates the least significant value for F at p<0.01.</p></div

Classification of genes annotated between the TTC8 and CiC3248-06 markers according to gene ontology (GO) functional categories.

<p>(A) GO biological process categories. (B) GO cellular component categories.</p

MOESM2 of De novo construction of a “Gene-space” for diploid plant genome rich in repetitive sequences by an iterative Process of Extraction and Assembly of NGS reads (iPEA protocol) with limited computing resources

Additional file 2. Mapping of genomics HiSeq 2000 reads against the pea Unigene at the first iteration

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Additional file 3. Evolution of the number and the size of the de novo genomics contigs as a function of the iterations

Adaptation of Maize to Temperate Climates: Mid-Density Genome-Wide Association Genetics and Diversity Patterns Reveal Key Genomic Regions, with a Major Contribution of the <i>Vgt2</i> (<i>ZCN8</i>) Locus

<div><p>The migration of maize from tropical to temperate climates was accompanied by a dramatic evolution in flowering time. To gain insight into the genetic architecture of this adaptive trait, we conducted a 50K SNP-based genome-wide association and diversity investigation on a panel of tropical and temperate American and European representatives. Eighteen genomic regions were associated with flowering time. The number of early alleles cumulated along these regions was highly correlated with flowering time. Polymorphism in the vicinity of the <i>ZCN8</i> gene, which is the closest maize homologue to <i>Arabidopsis</i> major flowering time (<i>FT</i>) gene, had the strongest effect. This polymorphism is in the vicinity of the causal factor of <i>Vgt2</i> QTL. Diversity was lower, whereas differentiation and LD were higher for associated loci compared to the rest of the genome, which is consistent with selection acting on flowering time during maize migration. Selection tests also revealed supplementary loci that were highly differentiated among groups and not associated with flowering time in our panel, whereas they were in other linkage-based studies. This suggests that allele fixation led to a lack of statistical power when structure and relatedness were taken into account in a linear mixed model. Complementary designs and analysis methods are necessary to unravel the architecture of complex traits. Based on linkage disequilibrium (LD) estimates corrected for population structure, we concluded that the number of SNPs genotyped should be at least doubled to capture all QTLs contributing to the genetic architecture of polygenic traits in this panel. These results show that maize flowering time is controlled by numerous QTLs of small additive effect and that strong polygenic selection occurred under cool climatic conditions. They should contribute to more efficient genomic predictions of flowering time and facilitate the dissemination of diverse maize genetic resources under a wide range of environments.</p></div

Manhattan plot for female flowering (FFLW8) associations across the whole genome.

<p><i>P</i>-values were obtained with the mixed model including the structure matrix obtained using STRUCTURE software and 55 SSRs and the kinship matrix obtained with 94 SSRs and IBS measure. Horizontal dashed line indicates Bonferroni-corrected 5% significance threshold.</p

Distribution of SNP effects (GDD) according to the early allele frequency.

<p>In this figure, 673 markers with <i>P</i>-value<10⁻³ are represented. (A) For all inbred lines (rows) and SNPs (columns), red and blue colors correspond to the presence of late and early alleles, respectively. (B) The absolute SNP effect versus the frequency of the early allele for significant associations. Different colors correspond to different <i>P</i>-value thresholds.</p