EF-P is essential for rapid synthesis of proteins containing consecutive proline residues

Elongation factor P (EF-P) is a translation factor of unknown function that has been implicated in a great variety of cellular processes. Here, we show that EF-P prevents ribosome from stalling during synthesis of proteins containing consecutive prolines, such as PPG, PPP, or longer proline strings, in natural and engineered model proteins. EF-P promotes peptide-bond formation and stabilizes the peptidyl–transfer RNA in the catalytic center of the ribosome. EF-P is posttranslationally modified by a hydroxylated b-lysine attached to a lysine residue. The modification enhances the catalytic proficiency of the factor mainly by increasing its affinity to the ribosome. We propose that EF-P and its eukaryotic homolog, eIF5A, are essential for the synthesis of a subset of proteins containing proline stretches in all cells

Spectroscopic determination of crystal field splittings in lanthanide double deckers

10.1039/c4sc00751dCHEMICAL SCIENCE583287-329

Fibers and fiber-optic components for high-power fiber lasers

The major challenge in the development of monolithic kW class CW fiber lasers is the efficient conversion of pump photons into a high brightness laser beam under the constraints of heat management, long term stability and nonlinearities. This article reviews the interaction of some fiber related aspects as e.g. fiber core composition, photodarkening and modality, as well as their influence on system complexity and power scalability. Recent work on active fibers, pump couplers, mode field adaptors and other fiber-optic components will be presented