Nederland en zijn veteranen 1945-2015: een onderzoek naar de rol van de overheid bij de ontwikkeling van het veteranenbeleid en de uitvoering van de veteranenzorg

Government has a special responsibility for its military. This research is about the care of veterans by government, parliament and society in the period 1945-2015. Therein the central matter is the development of the Dutch veteran policy (1945-2015) and in which way did the government met its responsibilities? To answer the main question, the theory of civil-military relations was used as a theoretical framework. This civil-military relationship concernsthe relationship between society and armed forces as well as the relationship between government and armed forces. Which roles did parliament and society fulfil in the care for veterans? Using six military missions, we researched in what ways the government met their duty of care towards (ex-)military personnel in practice before, during and after a mission.Until 1990 the government had no definition for a ‘veteran’. The military personnel who were deployed in the Dutch East Indies, Korea and the Lebanon as well as smaller missions, did not have veteran status before 1990.When viewing the period 1945-2015, we can conclude that the government did not meet fully its special responsibility to its veterans.Institutions, Decisions and Collective Behaviou

Two-dimensional model of dynamical fermion mass generation in strongly coupled gauge theories

We generalize the $N_F=2$ Schwinger model on the lattice by adding a charged scalar field. In this so-called $\chi U\phi_2$ model the scalar field shields the fermion charge, and a neutral fermion, acquiring mass dynamically, is present in the spectrum. We study numerically the mass of this fermion at various large fixed values of the gauge coupling by varying the effective four-fermion coupling, and find an indication that its scaling behavior is the same as that of the fermion mass in the chiral Gross-Neveu model. This suggests that the $\chi U\phi_2$ model is in the same universality class as the Gross-Neveu model, and thus renormalizable and asymptotic free at arbitrary strong gauge coupling.Comment: 18 pages, LaTeX2e, requires packages rotating.sty and curves.sty from CTA

Recombinant Modified Vaccinia Virus Ankara Expressing Glycoprotein E2 of Chikungunya Virus Protects AG129 Mice against Lethal Challenge

Chikungunya virus (CHIKV) infection is characterized by rash, acute high fever, chills, headache, nausea, photophobia, vomiting, and severe polyarthralgia. There is evidence that arthralgia can persist for years and result in long-term discomfort. Neurologic disease with fatal outcome has been documented, although at low incidences. The CHIKV RNA genome encodes five structural proteins (C, E1, E2, E3 and 6K). The E1 spike protein drives the fusion process within the cytoplasm, while the E2 protein is believed to interact with cellular receptors and therefore most probably constitutes the target of neutralizing antibodies. We have constructed recombinant Modified Vaccinia Ankara (MVA) expressing E3E2, 6KE1, or the entire CHIKV envelope polyprotein cassette E3E26KE1. MVA is an appropriate platform because of its demonstrated clinical safety and its suitability for expression of various heterologous proteins. After completing the immunization scheme, animals were challenged with CHIV-S27. Immunization of AG129 mice with MVAs expressing E2 or E3E26KE1 elicited neutralizing antibodies in all animals and provided 100% protection against lethal disease. In contrast, 75% of the animals immunized with 6KE1 were protected against lethal infection. In conclusion, MVA expressing the glycoprotein E2 of CHIKV represents as an immunogenic and effective candidate vaccine against CHIKV infections

Hamiltonian lattice QCD at finite density: equation of state in the strong coupling limit

The equation of state of Hamiltonian lattice QCD at finite density is examined in the strong coupling limit by constructing a solution to the equation of motion corresponding to an effective Hamiltonian describing the ground state of the many body system. This solution exactly diagonalizes the Hamiltonian to second order in field operators for all densities and is used to evaluate the vacuum energy density from which we obtain the equation of state. We find that up to and beyond the chiral symmetry restoration density the pressure of the quark Fermi sea can be negative indicating its mechanical instability. Our result is in qualitative agreement with continuum models and should be verifiable by future lattice simulations of strongly coupled QCD at finite density.Comment: 27 pages, 6 figures. Uses ReVTeX4 and BiBTeX. Revised versio

A novel antigen capture ELISA for the specific detection of IgG antibodies to elephant endotheliotropic herpes virus

BACKGROUND Elephants are classified as critically endangered animals by the International Union for Conservation of Species (IUCN). Elephant endotheliotropic herpesvirus (EEHV) poses a large threat to breeding programs of captive Asian elephants by causing fatal haemorrhagic disease. EEHV infection is detected by PCR in samples from both clinically ill and asymptomatic elephants with an active infection, whereas latent carriers can be distinguished exclusively via serological assays. To date, identification of latent carriers has been challenging, since there are no serological assays capable of detecting seropositive elephants. RESULTS Here we describe a novel ELISA that specifically detects EEHV antibodies circulating in Asian elephant plasma/serum. Approximately 80 % of PCR positive elephants display EEHV-specific antibodies. Monitoring three Asian elephant herds from European zoos revealed that the serostatus of elephants within a herd varied from non-detectable to high titers. The antibody titers showed typical herpes-like rise-and-fall patterns in time which occur in all seropositive animals in the herd more or less simultaneously. CONCLUSIONS This study shows that the developed ELISA is suitable to detect antibodies specific to EEHV. It allows study of EEHV seroprevalence in Asian elephants. Results confirm that EEHV prevalence among Asian elephants (whether captive-born or wild-caught) is high

Intestinal Tropism of a Betacoronavirus (Merbecovirus) in Nathusius’s Pipistrelle Bat (Pipistrellus nathusii), Its Natural Host

The emergence of several bat coronavirus-related disease outbreaks in human and domestic animals has fueled surveillance of coronaviruses in bats worldwide. However, little is known about how these viruses interact with their natural hosts. We demonstrate a Betacoronavirus (subgenus Merbecovirus), PN-βCoV, in the intestine of its natural host, Nathusius’s Pipistrelle Bat (Pipistrellus nathusii), by combining molecular and microscopy techniques. Eighty-eight P. nathusii bat carcasses were tested for PN-βCoV RNA by RT-qPCR, of which 25 bats (28%) tested positive. PN-βCoV RNA was more often detected in samples of the intestinal tract than in other sample types. In addition, viral RNA loads were higher in intestinal samples compared to other sample types, both on average and in each individual bat. In one bat, we demonstrated Merbecovirus antigen and PN-βCoV RNA expression in intestinal epithelium and the underlying connective tissue using immunohistochemistry and in situ hybridization, respectively. These results indicate that PN-βCoV has a tropism for the intestinal epithelium of its natural host, Nathusius’s Pipistrelle Bat, and imply that the fecal-oral route is a possible route of transmission.</p

Zika virus infection perturbs osteoblast function

Zika virus (ZIKV) infection is typically characterized by a mild self-limiting disease presenting with fever, rash, myalgia and arthralgia and severe fetal complications during pregnancy such as microcephaly, subcortical calcifications and arthrogyropsis. Virus-induced arthralgia due to perturbed osteoblast function has been described for other arboviruses. In case of ZIKV infection, the role of osteoblasts in ZIKV pathogenesis and bone related pathology remains unknown. Here, we study the effect of ZIKV infection on osteoblast differentiation, maturation and function by quantifying activity and gene expression of key biomarkers, using human bone marrow-derived mesenchymal stromal cells (MSCs, osteoblast precursors). MSCs were induced to differentiate into osteoblasts and we found that osteoblasts were highly susceptible to ZIKV infection. While infection did not cause a cytopathic effect, a significant reduction of key osteogenic markers such as ALP, RUNX2, calcium contents and increased expression of IL6 in ZIKV-infected MSCs implicated a delay in osteoblast development and maturation, as compared to uninfected controls. In conclusion, we have developed and characterized a new in vitro model to study the role of bone development in ZIKV pathogenesis, which will help to identify possible new targets for developing therapeutic and preventive measures

Index Theorem and Overlap Formalism with Naive and Minimally Doubled Fermions

We present a theoretical foundation for the Index theorem in naive and minimally doubled lattice fermions by studying the spectral flow of a Hermitean version of Dirac operators. We utilize the point splitting method to implement flavored mass terms, which play an important role in constructing proper Hermitean operators. We show the spectral flow correctly detects the index of the would-be zero modes which is determined by gauge field topology. Using the flavored mass terms, we present new types of overlap fermions from the naive fermion kernels, with a number of flavors that depends on the choice of the mass terms. We succeed to obtain a single-flavor naive overlap fermion which maintains hypercubic symmetry.Comment: 27 pages, 17 figures; references added, version accepted in JHE

The staggered domain wall fermion method

A different lattice fermion method is introduced. Staggered domain wall fermions are defined in 2n+1 dimensions and describe 2^n flavors of light lattice fermions with exact U(1) x U(1) chiral symmetry in 2n dimensions. As the size of the extra dimension becomes large, 2^n chiral flavors with the same chiral charge are expected to be localized on each boundary and the full SU(2^n) x SU(2^n) flavor chiral symmetry is expected to be recovered. SDWF give a different perspective into the inherent flavor mixing of lattice fermions and by design present an advantage for numerical simulations of lattice QCD thermodynamics. The chiral and topological index properties of the SDWF Dirac operator are investigated. And, there is a surprise ending...Comment: revtex4, 7 figures, minor revisions, 2 references adde