The Effect of Dispersed Oil on the Calcification Rate of the Reef-Building Coral Diploria Strigosa

Hermatypic corals represent environmentally and economically important components of the reef ecosystem. Oil spills and clean-up operations in reef areas are potential sources of pollution impact. This paper presents an evaluation of the calcification rate of specimens of the reef-building coral Diploria strigosa in response to 24 hour treatments of chemically dispersed oil at concentrations of 20 ppm. The concentrations and durations were chosen to represent a scenario of a short-term oil spill treated with dispersant passing over a coral reef. Calcification rates were determined by the buoyant weight technique at several day intervals for up to 29 days following treatment. Results from laboratory experiments (Winter and Summer) conducted in a flow-through seawater system indicate that treated corals, both in comparison to untreated controls as well as to their pretreatment rates, experienced no depression in calcification. In contrast, a possible short-term enhancement of calcification for the treated corals was observed

The Effects of Oil and Oil Dispersants on the Skeletal Growth of the Hermatypic Coral Diploria strigosa

Specimens of the hermatypic coral species Diploria strigosa were exposed to various concentrations (1–50 ppm) of oil or oil plus dispersant for 6–24 h periods in four laboratory and two field experiments. After dosing, corals were transplanted to, or left in, the field and recollected approximately one year later for extension (linear) growth analysis by the alizarin stain method. The experiments were designed to assess the long-term effects of brief low-level concentrations of chemically dispersed oil and oil alone on corals in a situation, for example, where an oil slick (treated and non-treated with dispersants) passes over a reef. No significant differences between extension growth parameters (Septa increase, Columella increase) and a calical shape parameter (New Endotheca Length) of treated corals versus controls were found in any of the experiments. In two summer experiments calical relief (Fossa length) was found to be depressed in corals of some of the experimental treatments

Behavioural Effects of Chemically Dispersed Oil and Subsequent Recovery in Diploria strigosa (Dana)

Survival and behaviour of the hermatypic coral Diploria strigosa was studied during 6–24 h doses with water-accomodated fractions of chemically dispersed crude oil, and for a subsequent recovery period of 1 month. Experiments utilized a flow-through laboratory dosing procedure and incorporated petroleum hydrocarbon measurements in order to simulate a major but short-term oil spill in shallow subtidal benthic reef environments. Chemically dispersed oil treatments consisted of Arabian Light Crude oil with Corexit 9527 or BP1100WD at 1–20 ppm concentrations of oil. In general, effects observed were sub-lethal, temporary, and associated with the highest concentrations tested. Responses to the presence of dispersed oil at 20ppm for 24 h included mesenterial filament extrusion, extreme tissue contraction, tentacle retraction and localized tissue rupture. The nature and severity of reactions during the dosing phase varied between colonies and treatments, but colonies typically resumed normal behaviour within 2 h to 4 d of the recovery period. It therefore seems unlikely that observed biological effects would impair long-term viability

The effect of HIV infection and HCV viremia on Inflammatory Mediators and Hepatic Injury-The Women\u27s Interagency HIV Study.

Hepatitis C virus infection induces inflammation and while it is believed that HIV co-infection enhances this response, HIV control may reduce inflammation and liver fibrosis in resolved or viremic HCV infection. Measurement of systemic biomarkers in co-infection could help define the mechanism of inflammation on fibrosis and determine if HIV control reduces liver pathology. A nested case-control study was performed to explore the relationship of systemic biomarkers of inflammation with liver fibrosis in HCV viremic and/or seropositive women with and without HIV infection. Serum cytokines, chemokines, growth factors and cell adhesion molecules were measured in HIV uninfected (HIV-, n = 18), ART-treated HIV-controlled (ARTc, n = 20), uncontrolled on anti-retroviral therapy (ARTuc, n = 21) and elite HIV controllers (Elite, n = 20). All were HCV seroreactive and had either resolved (HCV RNA-; \u3c50IU/mL) or had chronic HCV infection (HCV RNA+). In HCV and HIV groups, aspartate aminotransferase to platelet ratio (APRI) was measured and compared to serum cytokines, chemokines, growth factors and cell adhesion molecules. APRI correlated with sVCAM, sICAM, IL-10, and IP-10 levels and inversely correlated with EGF, IL-17, TGF-α and MMP-9 levels. Collectively, all HCV RNA+ subjects had higher sVCAM, sICAM and IP-10 compared to HCV RNA-. In the ART-treated HCV RNA+ groups, TNF-α, GRO, IP-10, MCP-1 and MDC were higher than HIV-, Elite or both. In ARTuc, FGF-2, MPO, soluble E-selectin, MMP-9, IL-17, GM-CSF and TGF-α are lower than HIV-, Elite or both. Differential expression of soluble markers may reveal mechanisms of pathogenesis or possibly reduction of fibrosis in HCV/HIV co-infection

Rare coding variants in ten genes confer substantial risk for schizophrenia

Rare coding variation has historically provided the most direct connections between gene function and disease pathogenesis. By meta-analysing the whole exomes of 24,248 schizophrenia cases and 97,322 controls, we implicate ultra-rare coding variants (URVs) in 10 genes as conferring substantial risk for schizophrenia (odds ratios of 3-50, PPeer reviewe

Large-scale sequencing identifies multiple genes and rare variants associated with Crohn’s disease susceptibility

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