Staphylococcal cassette chromosome mec typing and meca sequencing in methicillin-resistant staphylococci from Algeria: A highly diversified element with new mutations in mecA

Genetic mechanisms of methicillin resistance are still relevant in staphylococci. The aims of this study are to assess the possible exchanges of staphylococcal cassette chromosome mec (SCCmec) among isolates of methicillin-resistant staphylococci (MRS) and to check for known or new mutations in mecA DNA. A total of 35 MRS non-repetitive isolates were recovered, including 20 Staphylococcus haemolyticus, 7 Staphylococcus aureus, 4 Staphylococcus sciuri, 2 Staphylococcus saprophyticus and 1 isolate each of Staphylococcus xylosus and Staphylococcus lentus. Only 16 of the 35 strains were assigned to known SCCmec types: 7 SCCmec VII, 6 SCCmec IV and 3 SCCmec III, with possible horizontal transfer of the SCCmec VII from methicillin-resistant S. haemolyticus to methicillin-susceptible S. aureus. mecA gene sequencing in ten selected isolates allowed description of nine punctual mutations, seven of which were reported for the first time. The most frequent mutation was G246E, identified in isolates of methicillin-resistant S. aureus, S. sciuri, S. saprophyticus and S. lentus. These results emphasized the high degree of genetic diversity of SCCmec element in MRS and describe new missense mutations in mecA, which might be important in understanding the evolution of methicillin and new b-lactam resistance

Descriptive Epidemiology of Nasal Carriage of Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus Among Patients Admitted to Two Healthcare Facilities in Algeria

Aim: To evaluate nasal carriage rate and variables associated with Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) in patients admitted in two healthcare facilities. Results: S. aureus was isolated from 159 (26%) of the enrolled patients. Methicillin-susceptible S. aureus was isolated from 150 (24.5%) patients, and MRSA was isolated from 9 (1.5%). Cancer and previous hospitalization were associated with a significantly higher frequency of nasal S. aureus carriage among the patients admitted to the general hospital and the nephrology department, respectively. MRSA isolates were heterogeneous with respect to their staphylococcal cassette chromosome mec element (SCCmec) type, sequence type (ST), and toxin genes (pvl and tst1) content. Four isolates were attributed with the ST80-MRSA-IV clone, which is known to be predominant in Algeria. Conclusions: This is the first assessment of S. aureus and MRSA nasal carriage and associated variables in Algeria. Our findings provide also a picture of the MRSA strains circulating in the community in this geographic area. They can be useful as a guide for implementing screening and control procedures against S. aureus/MRSA in the Algerian healthcare facilities

Hyaluronic Acid Scaffolds for Loco-Regional Therapy in Nervous System Related Disorders

Hyaluronic acid (HA) is a Glycosaminoglycan made of disaccharide units containing N-acetyl-D-glucosamine and glucuronic acid. Its molecular mass can reach 10 MDa and its physiological properties depend on its polymeric property, polyelectrolyte feature and viscous nature. HA is a ubiquitous compound found in almost all biological tissues and fluids. So far, HA grades are produced by biotechnology processes, while in the human organism it is a major component of the extracellular matrix (ECM) in brain tissue, synovial fluid, vitreous humor, cartilage and skin. Indeed, HA is capable of forming hydrogels, polymer crosslinked networks that are very hygroscopic. Based on these considerations, we propose an overview of HA-based scaffolds developed for brain cancer treatment, central and peripheral nervous systems, discuss their relevance and identify the most successful developed systems

Panton-Valentine leukocidin positive sequence type 80 methicillin-resistant Staphylococcus aureus carrying a staphylococcal cassette chromosome mec type IVc is dominant in neonates and children in an Algiers hospital

Methicillin-resistant Staphylococcus aureus (MRSA) is a major antimicrobial drug-resistant pathogen causing serious infections. It was first detected in healthcare settings, but in recent years it has also become disseminated in the community. Children and young adults are most susceptible to infection by community-acquired (CA) MRSA strains. In this study 25 MRSA isolates implicated in infections of neonates and children admitted to an Algiers hospital during an 18 month period were characterized by molecular methods including staphylococcal cassette chromosome (SCC) mec typing, PCR amplification of pvl genes, pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Fifteen out of 25 isolates were from hospital-acquired infections. Twenty-four isolates carried SCCmec type IVc and belonged to the sequence type (ST) 80, one isolate carried SCCmec type II and was ST 39. Twenty-two out of 24 ST80-MRSA-IVc isolates carried pvl genes. Our results suggest that the Panton-Valentine leukocidin positive ST80- MRSA-IVc is the dominant MRSA clone causing disease in neonates and children in Algiers

Do About Half the Top Quarks at FNAL Come From Gluino Decays?

We argue that it is possible to make a consistent picture of FNAL data including the production and decay of gluinos and squarks. The additional cross section is several pb, about the size of that for Standard Model (SM) top quark pair production. If the stop squark mass is small enough, about half of the top quarks decay to stop squarks, and the loss of SM top quark pair production rate is compensated by the supersymmetric processes. This behavior is consistent with the reported top quark decay rates in various modes and other aspects of the data, and suggests several other possible decay signatures. This picture can be tested easily with more data, perhaps even with the data in hand, and demonstrates the potential power of a hadron collider to determine supersymmetric parameters. It also has implications for the top mass measurement and the interpretation of the LEP $R_b$ excess.Comment: 18 pages, including 4 Postscript figures, uses epsf.tex, also available at http://www.hep.anl.gov/theory/mrenna

Analysis of the dust evolution in the circumstellar disks of TTauri stars

We present a compositional analysis of 8-13um spectra of 32 young stellar objects (YSOs). Our sample consists of 5 intermediate-mass stars and 27 low-mass stars. While the spectra and first scientific results have already been published by Przygodda et al. (2003) and Kessler-Silacci et al. (2004) we perform a more detailed analysis of the 10um silicate feature. In our analysis we assume that this emission feature can be represented by a linear superposition of the wavelength-dependent opacity $\kappa_{\rm abs}(\lambda)$ describing the optical properties of silicate grains with different chemical composition, structure, and grain size. The determination of an adequate fitting equation is another goal of this study. Using a restricted number of fitting parameters we investigate which silicate species are necessary for the compositional fitting. Particles with radii of 0.1um- and 1.5um consisting of amorphous olivine and pyroxene, forsterite, enstatite, and quartz have been considered. Only compact, homogeneous dust grains have been used in the presented fitting procedures. In this context we show that acceptable fitting results can also be achieved if emission properties of porous silicate grains are considered instead. Although some previous studies give reasons for the similarity between the dust in circumstellar disks of TTauri stars and Herbig Ae/Be stars, a quantitative comparison has been missing, so far. Therefore, we conclude with a discussion of the results of a 10um spectroscopic survey of van Boekel et al. (2005) who focus on Herbig Ae/Be stars, the higher mass counterparts of T Tauri stars and draw comparisons to this and other studies. We find that the results of our study of T Tauri systems partly agree with previous studies of Herbig Ae/Be stars.Comment: 17 pages, 6 figure