SKRINING POTENSI PROBIOTIK DAN SITOTOKSIK BAKTERI Weisella confusa ISOLAT DANGKE SAPI

Kasus kanker kolorektal mengalami peningkatan setiap tahunnya di seluruh dunia. Salah satu upaya pencegahan kanker kolorektal ialah melalui konsumsi probiotik. Weisella confusa tergolong dalam kelompok bakteri asam laktat, isolasi bakteri ini dangke merupakan hal yang baru dan aktivitas probiotik dari isolat ini belum diketahui. Penelitian ini bertujuan menguji sifat probiotik W. confusa isolat dangke dan potensi sitotoksiknya terhadap larva udang Artemia salina sebagai skrining awal efek antikanker. Metode pengujian sifat probiotik meliputi uji toleransi terhadap pH rendah dan uji toleransi terhadap garam empedu. Skrining efek sitotoksik dilakukan menggunakan metode BSLT(Brine Shrimp Lethality Test) menggunakan hasil fermentasi 72 jam dari isolat. Berdasarkan hasil pengujian, W. confusa isolat dangke menunjukkan sifat toleran terhadap pH 2 dan garam empedu 0,3%. Hasil uji sitotoksikmenunjukkan bahwa filtrat hasil fermentasi memiliki nilai LC50 lebih dari 1000 µg/ml yang menandakan sampel tidak berefek sitotoksik.Disimpulkan bahwabakteri W.confusa isolat dangke menunjukkan potensi sebagai probiotik namun tidak bersifat sitotoksik sehingga tidak berpotensi untuk dilanjutkan pada uji antiproliferasi terhadap sel kanker. Pengujian aktivitas biologis lainnya disarankan untuk mengetahui potensi lain dari bakteri ini

ANALISIS RESIDU PESTISIDA KLORPIRIFOS PADA BERAS (Oryza sativa) YANG BERASAL KECAMATAN BAEBUNTA KABUPATEN LUWU UTARA

Penggunaan pestisida di Indonesia sudah merupakan bagian yang tidak terpisahkan dalam sistem pertanian. Klorpirifos merupakan salah satu kelompok pestisida golongan organofosfat yang banyak digunakan untuk membunuh berbagai serangga. Adanya residu klorpirifos dapat menyebabkan gangguan kesehatan sehingga deteksi residu klorpirifos dalam produk pangan merupakan hal yang penting. Penelitian ini dilakukan untuk mengetahui kadar residu klorpirifos pada beras yang berasal dari Kecamatan Baebunta, Kabupaten Luwu Utara dan keamanannya untuk dikonsumsi. Sampel beras diambil langsung dari beberapa petani di Kecamatan Baebunta, Kabupaten Luwu Utara. Sampel diekstraksi dan di clean-up menggunakan metode QuEChERS dan dianalisis menggunakan GC/MS. Kadar residu klorpirifos dibandingkan dengan standar Batas Maksimum Residu (BMR)pada SNI. Hasil penelitian menunjukkan bahwa residu klorpirifos pada sampel A, B dan C masing-masing sebesar 0,133; 0,092; dan 0,308 mg/kg. Dengan demikian, beras yang berasal dari Kecamatan Baebunta Kabupaten Luwu Utara memiliki kadar residu di bawah nilai BMR (0,5 mg/kg) sehingga aman untuk dikonsums

ASSESSMENT OF PROBIOTIC PROPERTIES AND SAFETY OF LACTIC ACID BACTERIA ISOLATED FROM SOUTH SULAWESI ETHNIC CHEESE

Probiotic bacteria have been used widely as a functional food and health supplements. The functionality and safety of probiotics are the prerequisites given by WHO (World Health Organization) and FAO (Food Agricultural Organization) before utilizing probiotics. This study focuses on assessing probiotic properties and the safety of the lactic acid bacteria (LAB) isolated from dangke—traditional cheese of South Sulawesi. In the current study, the assessment of probiotic properties was carried by assessing its tolerance against low pH and bile salts. Safety assessments were divided into two assays viz., susceptibility testing and hemolytic activity. LAB from dangke demonstrated tolerance against low pH, bile salt and susceptibility against four types of antibiotics (ampicillin, cefotaxime, erythromycin, and tetracycline). Unfortunately, resistance towards gentamycin and an a-hemolytic activity was observed. This bacterium met the functional criteria from probiotics but failed to meet the safety criteria for probiotic safety

Efek Sinergitas Ekstrak Kulit Jeruk (Citrus Sinensis L) pada Patch Bioselulosa dalam Meningkatkan Penyembuhan Luka Bakar: Synergetic Effect Of Orange Peel Extract In Biocellulose Patch Toward Burn Wound Healing

Burns is one of the incidents that can lead to death (mortality). One of the natural products that have  potential to serve as an alternative treatment of burns is orange peel. Orange peel has a chemical composition such as ascorbic acid, vitamin E, vitamin A, and polyphenols as antioxidants that inhibit free radicals responsible in the pathogenesis of both acute and chronic inflammatory. In this study, formulation was made in the form of biocellulose which is the primary metabolism product of bacteria.  The purpose of this research was to obtain the concentration of the extract of orange peel on bioselullose that have the effect of decreasing the burn wound in rats. Orange peel was extracted then fortified into biocellulose with a concentration of 3%, 6%, and 9%. After that, the wound healing was tested on animals in the form of decreasing the wound diameter.  The results showed that the concentration of extract of orange peel 3% on the fortification of biocellulose showed the good percentage of burn wound decreasing i.e. 45.52% with diameter average of 18.35 mm. This result indicates the concentration of extract of orange peel 3% is better than others

Inisiasi dan pengembangan produk handsanitizer pada center of excellence Fakultas Farmasi Universitas Hasanuddin sebagai upaya pengembangan usaha intelektual kampus

PPUPIK is a community service program for 3 years. The purpose of this activity is to produce the intellectual products from the Faculty of Pharmacy UNHAS, especially Hand sanitizer and Disinfectant products, so that the Center of Excellence of the Faculty of Pharmacy UNHAS (CoE-FFUH) would become an independent business unit that able to support UNHAS' role as PTNBH. The specific target for this first year is the initiation of the development of a production unit of hand sanitizer and disinfectant products at CoE-FFUH. The initiation consists of the determination of documents for the production process, determination of product packaging material and designs, and also pilot-scale production for limited sales. As for the following year, hopefully, this product will be able to obtain a distribution permit for household health supplies (PKRT) so that the commercialization of licensed products can be carried out legally and able to produce more types of PKRT products

Efek Sinergitas Ekstrak Kulit Jeruk (Citrus sinensis L) Pada Patch Bioselulosa Dalam Meningkatkan Penyembuhan Luka Bakar

Burns is one of the incidents that can lead to death (mortality). One of the natural products that have  potential to serve as an alternative treatment of burns is orange peel. Orange peel has a chemical composition such as ascorbic acid, vitamin E, vitamin A, and polyphenols as antioxidants that inhibit free radicals responsible in the pathogenesis of both acute and chronic inflammatory. In this study, formulation was made in the form of biocellulose which is the primary metabolism product of bacteria.  The purpose of this research was to obtain the concentration of the extract of orange peel on bioselullose that have the effect of decreasing the burn wound in rats. Orange peel was extracted then fortified into biocellulose with a concentration of 3%, 6%, and 9%. After that, the wound healing was tested on animals in the form of decreasing the wound diameter.  The results showed that the concentration of extract of orange peel 3% on the fortification of biocellulose showed the good percentage of burn wound decreasing i.e. 45.52% with diameter average of 18.35 mm. This result indicates the concentration of extract of orange peel 3% is better than others

Pharmaceutical approaches on antimicrobial resistance: prospects and challenges

The rapid increase in pathogenic microorganisms with antimicrobial resistant profiles has become a significant public health problem globally. The management of this issue using conventional antimicrobial preparations frequently results in an increase in pathogen resistance and a shortage of effective antimicrobials for future use against the same pathogens. In this review, we discuss the emergence of AMR and argue for the importance of addressing this issue by discovering novel synthetic or naturally occurring antibacterial compounds and providing insights into the application of various drug delivery approaches, delivered through numerous routes, in comparison with conventional delivery systems. In addition, we discuss the effectiveness of these delivery systems in different types of infectious diseases associated with antimicrobial resistance. Finally, future considerations in the development of highly effective antimicrobial delivery systems to combat antimicrobial resistance are presented

Progress and Challenges in Antimicrobial Resistance and Bacterial Vaccines

In recent decades, pathogens have continued to strike humans in the form of newly emerging or re-emerging infectious diseases, opportunistic infectious diseases, and infections caused by drug-resistant microbes. In response, humans have developed modern platform technologies that can produce effective vaccines to prevent pathogens from causing infectious diseases. Vaccines against antimicrobial-resistant organisms could prevent or minimize life-threatening infections, thus lowering healthcare costs. These pharmaceutical products could also reduce antibiotic use, lowering the risk of antimicrobial resistance (AMR) emergence. Furthermore, once a population has received enough vaccines, indirect protection via herd immunity can help to prevent the spread of resistant strains. In this sense, antibiotics would be unnecessary once the burden of pathogen-associated illnesses is reduced. Based on such a notion, bacterial vaccines would be an excellent and applicable solution to fight AMR. In this review, we highlight our current understanding of AMR, the role of bacterial vaccines in preventing AMR, and discuss the potential of bacterial vaccines and their pitfalls in managing infectious diseases