Craving Continuity from Cosmochemistry to Cosmochemists

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How experience influences infants’ recognition of male and female faces

Young infants with female primary caregivers are able to differentiate familiar female faces from novel female faces but not male faces. Experience processing faces may be important for being able to discriminate among similar-looking faces. Subsequently, increasing infants’ experience with less familiar faces should improve their ability to differentiate those types of faces. This study examined if infants’ experience with faces affected their recognition of new faces. Prior to testing, 2-3 month old infants were assigned to one of three conditions: a male video, a female video, and no video condition. Infants were familiarized to both male and female faces during test. For the male faces, infants who saw the male video showed a familiarity preference, infants who saw the female video showed a novelty preference, and infants who saw no video showed no preference. For female faces, infants showed no preference when assigned to the male video and no video condition, while infants assigned to the female video (n = 5) showed a familiarity preference. A follow up infant-controlled habituation study tested if infants processed faces featurally or holistically. During testing, infants saw one familiar face, one composite face, and one novel face. None of the infants in the male video, female video, or no video conditions were able to distinguish the familiar face from the composite face. Only infants in the female video condition showed an increase in looking time from the familiar face to the novel face

Spin observables of the reactions NN -> DeltaN and pd -> Delta (pp)(1S0) in collinear kinematics

A general formalism for double and triple spin-correlations of the reaction NN -> DeltaN is developed for the case of collinear kinematics. A complete polarization experiment allowing to reconstruct all of the four amplitudes describing this process is suggested. Furthermore, the spin observables of the inelastic charge-exchange reaction pd -> Delta^0(pp)(1S0) are analyzed in collinear kinematics within the single pN scattering mechanism involving the subprocess pn -> Delta^0p. The full set of spin observables related to the polarization of one or two initial particles and one final particle is obtained in terms of three invariant amplitudes of the reaction pd -> Delta (pp)(1S0) and the transition form factor d->(pp)(1S0). A complete polarization experiment for the reaction pd -> Delta^0(pp)(1S0) is suggested which allows one to determine three independent combinations of the four amplitudes of the elementary subprocess NN -> DeltaN.Comment: 12 pages, 1 figur

A search for new particles in proton‐nucleus collisions at 400 GeV/c

We report preliminary results from a search for new particles produced in proton‐nucleus collisions at 400 GeV/c. A double‐arm spectrometer is used to detect two‐body final states where each spectrometer arm has the capability of uniquely identifying Π±, K±, p, ?, μ±, and ϕ. The Jψ is measured in the μ+μ− mode.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87398/2/30_1.pd

An updated analysis of NN elastic scattering data to 1.6 GeV

An energy-dependent and set of single-energy partial-wave analyses of $NN$ elastic scattering data have been completed. The fit to 1.6~GeV has been supplemented with a low-energy analysis to 400 MeV. Using the low-energy fit, we study the sensitivity of our analysis to the choice of $\pi NN$ coupling constant. We also comment on the possibility of fitting $np$ data alone. These results are compared with those found in the recent Nijmegen analyses. (Figures may be obtained from the authors upon request.)Comment: 17 pages of text, VPI-CAPS-7/

Single-Molecule Imaging of an in Vitro-Evolved RNA Aptamer Reveals Homogeneous Ligand Binding Kinetics

Many studies of RNA folding and catalysis have revealed conformational heterogeneity, metastable folding intermediates, and long-lived states with distinct catalytic activities. We have developed a single-molecule imaging approach for investigating the functional heterogeneity of in vitro-evolved RNA aptamers. Monitoring the association of fluorescently labeled ligands with individual RNA aptamer molecules has allowed us to record binding events over the course of multiple days, thus providing sufficient statistics to quantitatively define the kinetic properties at the single-molecule level. The ligand binding kinetics of the highly optimized RNA aptamer studied here displays a remarkable degree of uniformity and lack of memory. Such homogeneous behavior is quite different from the heterogeneity seen in previous single-molecule studies of naturally derived RNA and protein enzymes. The single-molecule methods we describe may be of use in analyzing the distribution of functional molecules in heterogeneous evolving populations or even in unselected samples of random sequences

Experimental Facilities Development

This research was sponsored by the National Science Foundation Grant NSF PHy 87-1440

Conformational Dynamics of Single pre-mRNA Molecules During \u3cem\u3eIn Vitro\u3c/em\u3e Splicing

The spliceosome is a complex small nuclear RNA (snRNA)-protein machine that removes introns from pre-mRNAs via two successive phosphoryl transfer reactions. The chemical steps are isoenergetic, yet splicing requires at least eight RNA-dependent ATPases responsible for substantial conformational rearrangements. To comprehensively monitor pre-mRNA conformational dynamics, we developed a strategy for single-molecule FRET (smFRET) that uses a small, efficiently spliced yeast pre-mRNA, Ubc4, in which donor and acceptor fluorophores are placed in the exons adjacent to the 5′ and 3′ splice sites. During splicing in vitro, we observed a multitude of generally reversible time-and ATP-dependent conformational transitions of individual pre-mRNAs. The conformational dynamics of branchpoint and 3′-splice site mutants differ from one another and from wild type. Because all transitions are reversible, spliceosome assembly appears to be occurring close to thermal equilibrium

Molecular Dynamics and Quantum Mechanics of RNA: Conformational and Chemical Change We Can Believe In

Structure and dynamics are both critical to RNA’s vital functions in biology. Numerous techniques can elucidate the structural dynamics of RNA, but computational approaches based on experimental data arguably hold the promise of providing the most detail. In this Account, we highlight areas wherein molecular dynamics (MD) and quantum mechanical (QM) techniques are applied to RNA, particularly in relation to complementary experimental studies

Broad-spectrum aptamer inhibitors of HIV reverse transcriptase closely mimic natural substrates

A detailed understanding of how aptamers recognize biological binding partners is of considerable importance in the development of oligonucleotide therapeutics. For antiviral nucleic acid aptamers, current models predict a correlation between broad-spectrum inhibition of viral proteins and suppression of emerging viral resistance, but there is little understanding of how aptamer structures contribute to recognition specificity. We previously established that two independent single-stranded DNA aptamers, R1T and RT1t49(−5), are potent inhibitors of reverse transcriptases (RTs) from diverse branches of the primate lentiviral family, including HIV-1, HIV-2 and SIV(cpz). In contrast, class 1 RNA pseudoknots, such as aptamer T1.1, are specific for RTs from only a few viral clades. Here, we map the binding interfaces of complexes formed between RT and aptamers R1T, RT1t49(−5) and T1.1, using mass spectrometry-based protein footprinting of RT and hydroxyl radical footprinting of the aptamers. These complementary methods reveal that the broad-spectrum aptamers make contacts throughout the primer-template binding cleft of RT. The double-stranded stems of these aptamers closely mimic natural substrates near the RNase H domain, while their binding within the polymerase domain significantly differs from RT substrates. These results inform our perspective on how sustained, broad-spectrum inhibition of RT can be achieved by aptamers