Regional scale simulation of atmospheric structure using VAS data

Investigations with both Subsynoptic Scale Model (SSM) and Limited Area Mesoscale Prediction System (LAMPS) are directed at model static initializations using Visible Infrared Spin Scan Radiometer Atmospheric Sounder (VAS) derived temperatures and moistures and also satellite derived winds. The general approach for each day's case study is similar. A 12-hour control forecast (no satellite data) is made 12Z-OZ, using a radiosonde analysis at 12Z as the initial field. For the satellite data experiments, reanalyses are performed at intermediate times, using VAS soundings or VAS soundings in combination with satellite winds. Forecasts are subsequently made from the reanalysis time(s) to the same validation hour (OZ) as the control forecasts. Comparisons are then made between the control forecast and satellite experiments

Development of a heptaplex PCR assay for identification of Staphylococcus aureus and CoNS with simultaneous detection of virulence and antibiotic resistance genes

Background Staphylococcal toxicity and antibiotic resistance (STAAR) have been menacing public health. Although vancomycin-resistant Staphylococcus aureus (VRSA) is currently not as widespread as methicillin-resistant S. aureus (MRSA), genome evolution of MRSA into VRSA, including strains engineered within the same patient under anti-staphylococcal therapy, may build up to future public health concern. To further complicate diagnosis, infection control and anti-microbial chemotherapy, non-sterile sites such as the nares and the skin could contain both S. aureus and coagulase-negative staphylococci (CoNS), either of which could harbour mecA the gene driving staphylococcal methicillin-resistance and required for MRSA-VRSA evolution. Results A new heptaplex PCR assay has been developed which simultaneously detects seven markers for: i) eubacteria (16S rRNA), ii) Staphylococcus genus (tuf), iii) Staphylococcus aureus (spa), iv) CoNS (cns), v) Panton-Valentine leukocidin (pvl), vi) methicillin resistance (mecA), and vii) vancomycin resistance (vanA). Following successful validation using 255 reference bacterial strains, applicability to analyse clinical samples was evaluated by direct amplification in spiked blood cultures (n = 89) which returned 100 % specificity, negative and positive predictive values. The new assay has LoD of 1.0x103 CFU/mL for the 16S rRNA marker and 1.0x104 CFU/mL for six other markers and completes cycling in less than one hour. Conclusion The speed, sensitivity (100 %), NPV (100 %) and PPV (100 %) suggest the new heptaplex PCR assay could be easily integrated into a routine diagnostic microbiology workflow. Detection of the cns marker allows for unique identification of CoNS in mono-microbial and in poly-microbial samples containing mixtures of CoNS and S. aureus without recourse to the conventional elimination approach which is ambiguous. In addition to the SA-CoNS differential diagnostic essence of the new assay, inclusion of vanA primers will allow microbiology laboratories to stay ahead of the emerging MRSA-VRSA evolution. To the best of our knowledge, the new heptaplex PCR assay is the most multiplexed among similar PCR-based assays for simultaneous detection of STAAR

Astronomy And Astrophysics

. We consider a jet-disk symbiosis model to explain Gamma Ray Bursts and their afterglows. It is proposed that GRBs are created inside a pre-existing jet from a neutron star which collapses to a black hole due to massive accretion. In our model we assume that the initial energy due to this transition is all deposited in the jet by magnetic fields, using fully well explored concepts from jets and disks in active galactic nuclei and compact active stars in binary systems. The observed emission is then due to an ultrarelativistic shock wave propagating along the jet. We show that a good agreement between model predictions and observational data can be obtained for systems with accretion rates as high as in the Galactic jet source SS433. Specifically, we are able to reproduce the typical observed afterglow emission flux and its spectrum as a function of time. Key words: Gamma ray : bursts -- Shock waves -- ISM : jets and outflous -- Radiation mechanism : non-thermal 1. Introduction Gamma..