Evaluatie opvangbeleid 2005-2008 overwinterende ganzen en smienten. Deelrapport 8. Opvangkosten en inpasbaarheid op bedrijfsniveau

Dit rapport biedt inzicht in factoren die volgens boeren hun deelnamebereidheid bepalen aan de Provinciale Subsidieregeling Agrarisch Natuurbeheer (PSAN) voor ganzen via Programma Beheer van Dienst Regelingen. Een enquête is gehouden onder 386 agrarische bedrijven in provincie Frieslan

Dynamics of gene silencing during X inactivation using allele-specific RNA-seq

Background: During early embryonic development, one of the two X chromosomes in mammalian female cells is inactivated to compensate for a potential imbalance in transcript levels with male cells, which contain a single X chromosome. Here, we use mouse female embryonic stem cells (ESCs) with non-random X chromosome inactivation (XCI) and polymorphic X chromosomes to study the dynamics of gene silencing over the inactive X chromosome by high-resolution allele-specific RNA-seq. Results: Induction of XCI by differentiation of female ESCs shows that genes proximal to the X-inactivation center are silenced earlier than distal genes, while lowly expressed genes show faster XCI dynamics than highly expressed genes. The active X chromosome shows a minor but significant increase in gene activity during differentiation, resulting in complete dosage compensation in differentiated cell types. Genes escaping XCI show little or no silencing during early propagation of XCI. Allele-specific RNA-seq of neural progenitor cells generated from the female ESCs identifies three regions distal to the X-inactivation center that escape XCI. These regions, which stably escape during propagation and maintenance of XCI, coincide with topologically associating domains (TADs) as present in the female ESCs. Also, the previously characterized gene clusters escaping XCI in human fibroblasts correlate with TADs. Conclusions: The gene silencing observed during XCI provides further insight in the establishment of the repressive complex formed by the inactive X chromosome. The association of e

Miniaturised epigenome and interactome screening : development and application of microfluidic ChIP-seq and AP-MS

Contains fulltext : 213664.pdf (publisher's version ) (Open Access)Radboud University, 17 december 2019Promotor : Stunnenberg, H.G. Co-promotor : Marks, Hendrik203 p

Genome-wide epigenomic profiling for biomarker discovery

Contains fulltext : 165648.pdf (publisher's version ) (Open Access

Cell-autonomous TrkB signaling in presynaptic retinal ganglion cells mediates arbor growth and synapse maturation during the establishment of retinotectal synaptic connectivity

Contains fulltext : 35025.pdf (publisher's version ) (Open Access

Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements

Contains fulltext : 202828.pdf (publisher's version ) (Open Access

Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states

Contains fulltext : 201538.pdf (publisher's version ) (Open Access)21 p

Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency

Contains fulltext : 200091.pdf (Publisher’s version ) (Open Access

Erratum to: Dynamics of gene silencing during X inactivation using allele-specific RNA-seq

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Dynamics of gene silencing during X inactivation using allele-specific RNA-seq (vol 16, 149, 2015)

Contains fulltext : 157244.pdf (publisher's version ) (Open Access