Les produits de la mer au Sénégal et le potentiel des bactéries lactiques et des bactériocines pour la conservation

Fish preservation in Senegal: potential use of lactic acid bacteria and their antibacterial metabolites. In Senegal, fish are first as staple animal protein foods for populations. Socioeconomic constraints hamper the development of industrial preservations. Traditional fish production has some inherent food safety concerns considering the high susceptibility of seafood to bacterial spoilage. Some strategies of seafood products preservation using lactic acid bacteria producing bacteriocin are not very expensive and do not consume energy very much. They can be adapted in the preservation of fish in Senegal particularly in the fermentation in which the catches, generally handled for many hours at ambient temperature, are only added salt (NaCl) and sun dried. Lactic acid bacteria producing bacteriocins could be used as starters (with addition of carbohydrates) during the preparation of fish for rendering them more resistant to the growth of spoilage and pathogenic bacteria by in situ production of organic acids and bacteriocins. A second alternative of application of these bacteria is to use bactericidal solutions obtained from their cultures as additional barrier to sodium chloride to prevent growth of the flora during fish storage for maturation. These new strategies of preparation combined with drying can be used to enhance microbiological and dietetic qualities of local seafood commodities

Etude socio-économique et technologique de la production du poisson fermenté et séché (Guedj) au Sénégal

Cette étude aborde les aspects socio-économiques et technologiques relatifs à l’un des maillons très important de la pêche maritime qui est la transformation du poisson en guedj ou poisson fermenté et séché. En effet, une enquête diagnostique menée à travers trois zones de production au Sénégal (Dakar, Thiès et Fatick), combinée aux données de la bibliographie et des entretiens avec les acteurs, ont permis de faire ressortir un certain nombre d’enjeux. L’étude a montré que cette activité assurée à 80% par des femmes, génère beaucoup d’emplois et de revenus, que le prix de vente fluctue entre 1000 et 3500 frs CFA.kg-1 et par conséquent qu’une grande productrice peut gagner en moyenne jusqu’à 400000 frs CFA par mois. Cependant, malgré son importance économique, elle est confrontée à d’énormes difficultés liées à la manutention, au manque d’infrastructures et de stockage adéquates et aux techniques de transformation rudimentaires utilisées. Par ailleurs, l’analyse physicochimique et microbiologique réalisée sur des échantillons prélevés dans les sites de production et de vente laisse apparaître la nécessité d’appliquer rigoureusement les bonnes pratiques de fabrication et d’hygiène afin d’améliorer la qualité sanitaire du guedj, qui fait de plus en plus l’objet de demande du marché extérieur.Mots clés : Guedj, poisson fermenté, méthode traditionnelle, étude de filière, qualité, Sénégal

Effets de l’inoculation mycorhizienne sur le sesame (Sesamum indicum L.) en conditions naturelles

La présence des microorganismes symbiotiques dans le sol constitue un atout pour la majorité des cultures. L’inoculation avec des champignons mycorhiziens à arbuscules (CMA) est considérée comme un des moyens biologiques permettant à la culture de résister aux stress biotiques et abiotiques, et d’augmenter la productivité. Mais en conditions naturelles, la combinaison de plusieurs facteurs a une influence majeure dans l’établissement de la mycorhization. L’inoculation du sésame (Sesamum indicum L.) avec deux souches de Glomus est expérimentée en milieu naturel, dans un dispositif en split-plot avec quatre répétitions. Cetteinoculation mycorhizienne en milieu naturel a produit les effets suivants : le taux de mycorhization des plants inoculés reste bas ; l’inoculation n’a pas eu un effet significatif sur le rendement en graines, le nombre decapsules et la biomasse totale comparativement aux plants témoins naturellement infectés. Cela montre que, la mycorhization naturelle a eu une efficacité comparable à celle des souches inoculées sur le sesame. On peut déduire que les sols de cette zone de Bambey contiendraient des souches de champignons mycorhiziens à arbuscules (CMA) indigènes aussi efficaces que des souches sélectionnées.© 2013 International Formulae Group. All rights reserved.Mots clés : Mycorhization naturelle, Glomus mosseae, Glomus fasciculatum, sésame, productivité

Faible taux de succès du sevrage tabagique à court et moyen termes au décours d’un infarctus aigu du myocarde dans un service de cardiologie de Dakar au Sénégal

Introduction:Le tabagisme est un puissant facteur de risque cardio-vasculaire. Son sevrage semble moins bien pris en compte chez les coronariens. Les objectifs de ce travail étaient d’évaluer la prévalence du tabagisme et le sevrage tabagique au décours d’un infarctus du myocarde dans un service de cardiologie au Sénégal. Méthodes: Il s’agit d’une étude transversale et descriptive réalisée entre janvier 2008 et juin 2010. Nous avons recruté les patients hospitalisés pour infarctus du myocarde puis les fumeurs actifs ont été inclus dans notre enquête. Les malades étaient sensibilisés pour l’arrêt du tabac puis suivis à 3 mois, 6 mois et 12 mois pour évaluer le sevrage tabagique. Résultats: Nous avons recensé 82 patients hospitalisés pour un infarctus du myocarde, parmi eux 26 sujets (25 hommes) étaient fumeurs (31,7%). L’âge moyen des sujets fumeurs était de 56 ± 11,5 ans. La consommation moyenne de tabac était de 32 ± 14 paquets-année et le score moyen de Fagerström de 8. Tous les patients ont arrêté le tabac pendant l’hospitalisation. Après un suivi de 3 mois, 45% des patients ont repris le tabac, 65% à 6 mois et 85% à 12 mois. Conclusion: Le tabagisme est assez fréquent chez les patients sénégalais présentant un infarctus du myocarde. Le taux de sevrage tabagique à court et moyen termes est faible. Le sevrage tabagique devrait alors constituer un objectif privilégié dans la prévention des maladies cardio-vasculaires.Key words: Tabagisme, sevrage, coronaropathie, risque cardio-vasculaire, Senega

Inhibition of Fungi and Gram-Negative Bacteria by Bacteriocin BacTN635 Produced by Lactobacillus plantarum sp. TN635

The aim of this study was to evaluate 54 lactic acid bacteria (LAB) strains isolated from meat, fermented vegetables and dairy products for their capacity to produce antimicrobial activities against several bacteria and fungi. The strain designed TN635 has been selected for advanced studies. The supernatant culture of this strain inhibits the growth of all tested pathogenic including the four Gram-negative bacteria (Salmonella enterica ATCC43972, Pseudomonas aeruginosa ATCC 49189, Hafnia sp. and Serratia sp.) and the pathogenic fungus Candida tropicalis R2 CIP203. Based on the nucleotide sequence of the 16S rRNA gene of the strain TN635 (1,540 pb accession no FN252881) and the phylogenetic analysis, we propose the assignment of our new isolate bacterium as Lactobacillus plantarum sp. TN635 strain. Its antimicrobial compound was determined as a proteinaceous substance, stable to heat and to treatment with surfactants and organic solvents. Highest antimicrobial activity was found between pH 3 and 11 with an optimum at pH = 7. The BacTN635 was purified to homogeneity by a four-step protocol involving ammonium sulfate precipitation, centrifugal microconcentrators with a 10-kDa membrane cutoff, gel filtration Sephadex G-25, and C18 reverse-phase HPLC. SDS-PAGE analysis of the purified BacTN635, revealed a single band with an estimated molecular mass of approximately 4 kDa. The maximum bacteriocin production (5,000 AU/ml) was recorded after a 16-h incubation in Man, Rogosa, and Sharpe (MRS) medium at 30 °C. The mode of action of the partial purified BacTN635 was identified as bactericidal against Listeria ivanovii BUG 496 and as fungistatic against C. tropicalis R2 CIP203

Hepatitis B virus (HBV) infection amongst children in Senegal: current prevalence and seroprotection level

Introduction: hepatitis B virus (HBV) infection is highly endemic in Senegal. HBV vaccine of all children has been introduced in 1999 and included in the Expanded Programme on Immunization in 2004. The aim of this study was to assess the HBV prevalence and immunity status against HBV amongst children in Senegal. Methods: between March and August 2016, consecutive children aged from 6 months to 16 years old were recruited in outpatient department of three main children hospitals in Senegal. Serum samples were analyzed for HBV serology (HBsAg, HBcAb, HBsAb) using ARCHITECT analyzer. Children with HBsAb levels ≥ 10 IU/l) were considered as seroprotected against HBV. Results: during the study period, 295 children fulfilled the criteria for the study and were further analyzed. Three children were HBsAg positive giving a seroprevalence at 1.1% (95% CI: 0.2-3.3), 12/267 (4.5%, 95% CI=2.3-7.7) had positive HBcAb and 226/295 (76.6%, 71.4-81.3) had positive HBsAb including 191 (77.3%, 71.6-82.4) with isolated HBsAb related to previous active immunization. However only 165 children (56%, CI 50-62) had seroprotective HBsAb levels (HBsAb ≥ 10 UI/L) and 63 (21.4, 16.8-26) had a strong seroprotectiondefined by HBsAb ≥ 100 IU/L. Conclusion: our results suggest that although HBV prevalence has significantly decreased in children in Senegal following a better HBV vaccine coverage, the number of children correctly seroprotected is insufficient (56%). Assessing the levels of HBsAb and providing HBV vaccine boosters should be considered in children in Senegal

Stabilisation of Clay Mixtures and Soils by Alkali Activation

Alkali activation of clay minerals in soil can be used to form a stabilising phase, such as a geopolymer. Because this requires low-temperature curing, typically <100 °C, it has the potential to be a lower-energy method than fired brick or even cement or lime stabilisation. Whilst this has been demonstrated for individual clay minerals and soils, it is unknown how effective this stabilisation method is for naturally occurring soils from around the world. The potential for stabilisation by alkali activation of a given clay mixture or soil could be approximately, but not fully, predicted from its clay mineralogical composition

Regional research priorities in brain and nervous system disorders

The characteristics of neurological, psychiatric, developmental and substance-use disorders in low-and middle-income countries are unique and the burden that they have will be different from country to country. Many of the differences are explained by the wide variation in population demographics and size, poverty, conflict, culture, land area and quality, and genetics. Neurological, psychiatric, developmental and substance-use disorders that result from, or are worsened by, a lack of adequate nutrition and infectious disease still afflict much of sub-Saharan Africa, although disorders related to increasing longevity, such as stroke, are on the rise. In the Middle East and North Africa, major depressive disorders and post-traumatic stress disorder are a primary concern because of the conflict-ridden environment. Consanguinity is a serious concern that leads to the high prevalence of recessive disorders in the Middle East and North Africa and possibly other regions. The burden of these disorders in Latin American and Asian countries largely surrounds stroke and vascular disease, dementia and lifestyle factors that are influenced by genetics. Although much knowledge has been gained over the past 10 years, the epidemiology of the conditions in low-and middle-income countries still needs more research. Prevention and treatments could be better informed with more longitudinal studies of risk factors. Challenges and opportunities for ameliorating nervous-system disorders can benefit from both local and regional research collaborations. The lack of resources and infrastructure for health-care and related research, both in terms of personnel and equipment, along with the stigma associated with the physical or behavioural manifestations of some disorders have hampered progress in understanding the disease burden and improving brain health. Individual countries, and regions within countries, have specific needs in terms of research priorities.Fil: Ravindranath, Vijayalakshmi. Indian Institute of Science; IndiaFil: Dang, Hoang Minh. Vietnam National University; VietnamFil: Goya, Rodolfo Gustavo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata ; ArgentinaFil: Mansour, Hader. University of Pittsburgh; Estados Unidos. Mansoura University; EgiptoFil: Nimgaonkar, Vishwajit L.. University of Pittsburgh; Estados UnidosFil: Russell, Vivienne Ann. University of Cape Town; SudáfricaFil: Xin, Yu. Peking University; Chin