CliFEM - Climate Forcing and Erosion Modelling in the Sele River Basin (Southern Italy)

Abstract. This study presents a revised and scale-adapted Foster-Meyer-Onstad model (Foster et al., 1977) for the transport of soil erosion sediments under scarce input data, with the acronym CliFEM (Climate Forcing and Erosion Modelling). This new idea was addressed to develop a monthly time scale invariant Net Erosion model (NER), with the aim to consider the different erosion processes operating at different time scales in the Sele River Basin (South Italy), during 1973–2007 period. The sediment delivery ratio approach was applied to obtain an indirect estimate of the gross erosion too. The examined period was affected by a changeable weather regime, where extreme events may have contributed to exacerbate soil losses, although only the 19% of eroded sediment was delivered at outlet of the basin. The long-term average soil erosion was very high (73 Mg ha−1 per year ± 58 Mg ha−1). The estimate of monthly erosion showed catastrophic soil losses during the soil tillage season (August–October), with consequent land degradation of the hilly areas of the Sele River Basin

Western Mediterranean precipitation over the last 300 years from instrumental observations

The paper reports the results of the analysis of the 14 longest precipitation instrumental series, covering the last 300 years, that have been recovered in six subareas of the Western Mediterranean basin, i.e., Portugal, Northern and Southern Spain, Southern France, Northern and Southern Italy. This study extends back by one century our knowledge about the instrumental precipitation over theWestern Mediterranean, and by two centuries in some specific subareas. All the time series show repeated swings. No specific trends have been found over the whole period, except in a few cases, but with modest time changes and sometimes having opposite tendency. The same can be said for the most recent decades although with some more marked departures from the average. The correlation between the various Mediterranean subareas is generally not significant, or almost uncorrelated. The Wavelet Spectral Analysis applied to the precipitation identifies only a minor 56-year cycle in autumn, i.e., the same return period that has been found in literature for the Sea Surface Temperature over North Atlantic. A comparison with a gridded dataset reconstruction based on mixed multiproxy and instrumental observations, shows that the grid reconstruction is in good agreement with the observed data for the period after 1900, less for the previous period

Label-free fiber optic optrode for the detection of class C beta-lactamases expressed by drug resistant bacteria

This paper reports the experimental assessment of an automated optical assay based on label free optical fiber optrodes for the fast detection of class C beta-lactamases (AmpC BLs), actually considered as one of the most important sources of resistance to beta-lactams antibiotics expressed by resistant bacteria. Reflection-type long period fiber gratings (RT-LPG) have been used as highly sensitive label free optrodes, while a higher affine boronic acid based ligand was here selected to enhance the overall assay performances compared to those obtained in our first demonstration. In order to prove the feasibility analysis towards a fully automated optical assay, an engineered system was developed to simultaneously manipulate and interrogate multiple fiber optic optrodes in the different phases of the assay. The automated system tested in AmpC solutions at increasing concentrations demonstrated a limit of detection (LOD) of 6 nM, three times better when compared with the results obtained in our previous work. Moreover, the real effectiveness of the proposed optical assay has been also confirmed in complex matrices as the case of lysates of Escherichia coli overexpressing AmpC. (C) 2017 Optical Society of Americ

Highly dexterous 2-module soft robot for intra-organ navigation in minimally invasive surgery

Background: For some surgical interventions, like the Total Mesorectal Excision (TME), traditional laparoscopes lack the flexibility to safely maneuver and reach difficult surgical targets. This paper answers this need through designing, fabricating and modelling a highly dexterous 2-module soft robot for minimally invasive surgery (MIS). / Methods: A soft robotic approach is proposed that uses flexible fluidic actuators (FFAs) allowing highly dexterous and inherently safe navigation. Dexterity is provided by an optimized design of fluid chambers within the robot modules. Safe physical interaction is ensured by fabricating the entire structure by soft and compliant elastomers, resulting in a squeezable 2-module robot. An inner free lumen/chamber along the central axis serves as a guide of flexible endoscopic tools. A constant curvature based inverse kinematics model is also proposed, providing insight into the robot capabilities. / Results: Experimental tests in a surgical scenario using a cadaver model are reported, demonstrating the robot advantages over standard systems in a realistic MIS environment. / Conclusion: Simulations and experiments show the efficacy of the proposed soft robot

Not only dominant, not only optic atrophy: expanding the clinical spectrum associated with OPA1 mutations

Background: Heterozygous mutations in OPA1 are a common cause of autosomal dominant optic atrophy, sometimes associated with extra-ocular manifestations. Few cases harboring compound heterozygous OPA1 mutations have been described manifesting complex neurodegenerative disorders in addition to optic atrophy. Results: We report here three patients: one boy showing an early-onset mitochondrial disorder with hypotonia, ataxia and neuropathy that was severely progressive, leading to early death because of multiorgan failure; two unrelated sporadic girls manifesting a spastic ataxic syndrome associated with peripheral neuropathy and, only in one, optic atrophy. Using a targeted resequencing of 132 genes associated with mitochondrial disorders, in two probands we found compound heterozygous mutations in OPA1: in the first a 5 nucleotide deletion, causing a frameshift and insertion of a premature stop codon (p.Ser64Asnfs*7), and a missense change (p.Ile437Met), which has recently been reported to have clinical impact; in the second, a novel missense change (p.Val988Phe) co-occurred with the p.Ile437Met substitution. In the third patient a homozygous mutation, c.1180G > A (p.Ala394Thr) in OPA1 was detected by a trio-based whole exome sequencing approach. One of the patients presented also variants in mitochondrial DNA that may have contributed to the peculiar phenotype. The deleterious effect of the identified missense changes was experimentally validated in yeast model. OPA1 level was reduced in available patients\u2019 biological samples, and a clearly fragmented mitochondrial network was observed in patients\u2019 fibroblasts. Conclusions: This report provides evidence that bi-allelic OPA1 mutations may lead to complex and severe multi-system recessive mitochondrial disorders, where optic atrophy might not represent the main feature