Automated water source scheduling system with flow control system

Too much application of fertilizer can kill the plants and in irrigation, little by little application of fertilizer is better than one time big time fertilizing the plants. Water scarcity is also a major concern in irrigation. To solve the shortage of water sources, management and optimization of water sources is needed. The aim of this study is to develop a scheduling system of water sources: water with fertilizer, pond water, and ground water using Arduino and DS3231 Real Time Clock. Also, a flow control system is added to increase the efficiency of water use. The system was implemented in a controlled environmental chambers. Based on the result, the system design has been successfully functional and operational. © 2018 IEEE

Reproducibility of Oral Exam Grades and Correlation with Other Measures of Performance on Three Required Third-Year Clerkships

The oral examination is one of the traditional measures of student performance during clinical clerkships. Other studies have compared oral exams, written exams, and clinicalperformance, finding an unequal correlation among them and poor reproducibility of scores among examiners. This study of a third-year class on three required clerkships found a stronger correlation between oral exam performance and cumulative grade point average (GPA) than had previously been reported between oral exams and written or clinical grades and also found high reproducibility across clerkships, both overall and within class quartiles. These findings argue for wider use of the oral exam as an evaluation instrument on clinical clerkships.Yeshttps://us.sagepub.com/en-us/nam/manuscript-submission-guideline

Multiplexed Target Detection Using DNA-Binding Dye Chemistry in Droplet Digital PCR

Two years ago, we described the first droplet digital PCR (ddPCR) system aimed at empowering all researchers with a tool that removes the substantial uncertainties associated with using the analogue standard, quantitative real-time PCR (qPCR). This system enabled TaqMan hydrolysis probe-based assays for the absolute quantification of nucleic acids. Due to significant advancements in droplet chemistry and buoyed by the multiple benefits associated with dye-based target detection, we have created a “second generation” ddPCR system compatible with both TaqMan-probe and DNA-binding dye detection chemistries. Herein, we describe the operating characteristics of DNA-binding dye based ddPCR and offer a side-by-side comparison to TaqMan probe detection. By partitioning each sample prior to thermal cycling, we demonstrate that it is now possible to use a DNA-binding dye for the quantification of multiple target species from a single reaction. The increased resolution associated with partitioning also made it possible to visualize and account for signals arising from nonspecific amplification products. We expect that the ability to combine the precision of ddPCR with both DNA-binding dye and TaqMan probe detection chemistries will further enable the research community to answer complex and diverse genetic questions