Localization of soluble guanylyl cyclase in the superficial dorsal horn

Nitric oxide (NO) has been implicated in pain processing at the spinal level, but the mechanisms mediating its effects remain unclear. In the present work, we studied the organization of the major downstream effector of NO, soluble guanylyl cyclase (sGC), in the superficial dorsal horn of rat. Almost all neurokinin 1 (NK1) receptor-positive neurons in lamina I (a major source of ascending projections) were strongly immunopositive for sGC. Many local circuit neurons in laminae I-II also stained for sGC, but less intensely. Numerous fibers, presumably of unmyelinated primary afferent (C fiber) origin, stained for calcitonin gene-related peptide or isolectin B4, but none of these was immunopositive for sGC. These data, along with immunoelectron microscopy results, imply that unmyelinated primary afferent fibers terminating in the superficial dorsal horn lack sGC. Double labeling showed that neuronal nitric oxide synthase (nNOS) seldom colocalized with sGC, but nNOS-positive structures were frequently closely apposed to sGC-positive structures, suggesting that in the superficial dorsal horn NO acts mainly in a paracrine manner. Our data suggest that the NK1 receptor-positive projection neurons in lamina I are a major target of NO released in superficial dorsal horn. NO may also influence local circuit neurons, but it does not act on unmyelinated primary afferent terminals via sGC

CodeScore: Evaluating Code Generation by Learning Code Execution

A proper code evaluation metric (CEM) profoundly impacts the evolution of code generation, which is an important research field in NLP and software engineering. Prevailing CEMs can be categorized into match-based CEMs (e.g., BLEU, Accuracy, and CodeBLEU) and execution-based CEMs (e.g., AvgPassRatio and Pass@k), but both of them suffer from some issues. The former only measures differences in surface form regardless of the functional equivalence of codes, while the latter has huge execution overheads, including collecting expensive test cases, resolving tedious execution dependencies, and enormous execution time. To address these issues, in this paper, we propose CodeScore, an efficient and effective CEM for code generation, which estimates test case PassRatio of generated code without executing code. We also present a framework named UniCE for training unified code evaluation models by learning code execution, i.e., learning PassRatio and Executability of generated code. In order to learn code execution comprehensively, we construct more than 100 test cases for each task in several popular benchmark datasets, covering MBPP, APPS, and HumanEval. Experimental results show that CodeScore has obtained a state-of-the-art correlation with execution-based CEMs. CodeScore is strongly correlated with AvgPassPatio, and binary CodeScore is moderately correlated with Pass@1. In particular, CodeScore eliminates the need for test cases and execution dependencies in inference, and CodeScore reduces execution time by three orders of magnitude compared to AvgPassPatio and Pass@1

Robust model predictive control under redundant channel transmission with applications in networked DC motor systems

In networked systems, intermittent failures in data transmission are usually inevitable due to the limited bandwidth of the communication channel, and an effective countermeasure is to add redundance so as to improve the reliability of the communication service. This paper is concerned with the model predictive control (MPC) problem by using static output feedback for a class of polytopic uncertain systems with redundant channels under both input and output constraints. By utilizing the min-max control approach combined with stochastic analysis, sufficient conditions are established to guarantee the feasibility of the designed MPC scheme that ensures the robust stability of the closed-loop system. In terms of the solution to an auxiliary optimization problem, an easy-to-implement MPC algorithm is proposed to obtain the desired sub-optimal control sequence as well as the upper bound of the quadratic cost function. Finally, to illustrate its effectiveness, the proposed design method is applied to control a networked direct current motor system

Learning a Universal Human Prior for Dexterous Manipulation from Human Preference

Generating human-like behavior on robots is a great challenge especially in dexterous manipulation tasks with robotic hands. Even in simulation with no sample constraints, scripting controllers is intractable due to high degrees of freedom, and manual reward engineering can also be hard and lead to non-realistic motions. Leveraging the recent progress on Reinforcement Learning from Human Feedback (RLHF), we propose a framework to learn a universal human prior using direct human preference feedback over videos, for efficiently tuning the RL policy on 20 dual-hand robot manipulation tasks in simulation, without a single human demonstration. One task-agnostic reward model is trained through iteratively generating diverse polices and collecting human preference over the trajectories; it is then applied for regularizing the behavior of polices in the fine-tuning stage. Our method empirically demonstrates more human-like behaviors on robot hands in diverse tasks including even unseen tasks, indicating its generalization capability

IT USAGE BEHAVIOR OF MEDICAL PERSONNEL: AN EMPIRICAL STUDY BASED ON THE THEORY OF PLANNED BEHAVIOR

Digitalization of regional public health information is an irreversible trend in the course of China\u27s social development. This is also one of the key tasks to reform China\u27s medicine and public health system. To learn more about the influence of using information technology for medical personnel and hospital performance, this study reviews in the personal perspective to know more about the sustainable action of using IT in hospitals based on the Theory of Planned Bahavior. We proposed a research model which expanded TPB and tested it based on 278 valid data collected from employees in a large-scale hospital in Anhui Province, East China. The results of study reveal: 1.We verified TPB in the Chinese context and found that attitude and subjective norm not only have a directly influence on behavioral intention, but also can influence the use behavior.2.The habit of using IT of medical personnel has a directly influence on the attitude of using IT, and it also has significant influence on the behavioral intention. Our research model discovered the relationship between subjective factors of IT users’ and their behaviour. The founding of this study not only can provide references regarding the direction of public hospitals reform in China for decision makers of hospitals, but also is very helpful for management teams in hospitals to adopt more rational management measures for better IT use among medical personnel, and better hospital performance

Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production

Monocytes/macrophages play an important role in rheumatoid arthritis (RA) pathogenesis. They can activate fibroblasts through many molecules, including IL-1 and tumor necrosis factor-alpha, but there have been very few reports on the role of CD147 in RA. In our study, the results of flow cytometry reveal that the mean fluorescence intensity (MFI) of CD147 expression on CD14+ monocytes of peripheral blood from RA patients was higher than that in normal control and ankylosing spondylitis (AS) patients. The MFI of CD147 expression on the CD14+ monocytes in RA synovial fluid was higher than that in RA peripheral blood. Immunohistochemical staining shows that CD147 expression in RA synovium correlated with matrix metalloproteinase (MMP)-1 expression. A double immunofluorescent assay shows that CD147 was expressed on CD68+ cells in RA synovium. The potential role of CD147 in cyclophilin A (CyPA)-mediated cell migration was studied using a chemotaxis assay in vitro and it was found that the addition of anti-CD147 antibody or a CD147 antagonistic peptide significantly decreased the chemotactic index of the mononuclear cells. The role of CD147 in MMP production and cell invasion in vitro were studied through the co-culture of human CD14+ monocytes or monocytic line THP-1 cells and human fibroblasts, as well as by gel zymography and an invasion assay. Significantly elevated release and activation of MMP-9 and/or MMP-2 were seen in the co-culture of human monocytes/THP-1 cells and fibroblasts compared with cultures of the cells alone. An increased number of cells invading through the filters in the invasion assays was also observed in the co-cultured cells. The addition of CD147 antagonistic peptide had some inhibitory effect, not only on MMP production but also on cell invasion in the co-culture. Our study demonstrates that the increased expression of CD147 on monocytes/macrophages in RA may be responsible for elevated MMP secretion, cell invasion and CyPA-mediated cell migration into the joints, all of which may contribute to the cartilage and bone destruction of RA. These findings, together with a better understanding of CD147, CyPA and RA, will help in the development of innovative therapeutic interventions for RA

Subcellular organization of camkii in rat hippocampal pyramidal neurons: CaMKII in Dendritic Spines

Calcium/calmodulin-dependent protein kinase II (CaM-KII) plays a key role in N-methyl-D-aspartate (NMDA) receptor-dependent long-term synaptic plasticity; its location is critical for signal transduction, and may provide clues that further elucidate its function. We therefore examined the subcellular localization of CaMKII in CA1 stratum radiatum of adult rat hippocampus, by using immuno-electron microscopy after chemical fixation. When tissue was fixed quickly, the concentration of CaMKIIα (assessed by pre-embedding immunogold) was significantly higher in dendritic shafts than in spine heads. However, when tissue was fixed 5 minutes after perfusion with normal saline, the density of labeling decreased in dendritic shaft while increasing in spine heads, implying rapid translocation into the spine during brief perimortem stress. Likewise, in quickly fixed tissue, CaMKII within spine heads was found at comparable concentrations in the “proximal” half (adjacent to the spine neck) and the “distal” half (containing the postsynaptic density [PSD]), whereas after delayed fixation, label density increased in the distal side of the spine head, suggesting that CaMKII within the spine head moves toward the PSD during this interval. To estimate its distribution at the synapse in vivo, we performed postembedding immunogold staining for CaMKII in quick-fixed tissue, and found that the enzyme did not concentrate primarily within the central matrix of the PSD. Instead, labeling density peaked ∼40 nm inside the postsynaptic membrane, at the cytoplasmic fringe of the PSD. Labeling within 25 nm of the postsynaptic membrane concentrated at the lateral edge of the synapse. This lateral “PSD core” pool of CaMKII may play a special role in synaptic plasticity