Comparative Genomics and Transcriptomics To Analyze Fruiting Body Development in Filamentous Ascomycetes.

Many filamentous ascomycetes develop three-dimensional fruiting bodies for production and dispersal of sexual spores. Fruiting bodies are among the most complex structures differentiated by ascomycetes; however, the molecular mechanisms underlying this process are insufficiently understood. Previous comparative transcriptomics analyses of fruiting body development in different ascomycetes suggested that there might be a core set of genes that are transcriptionally regulated in a similar manner across species. Conserved patterns of gene expression can be indicative of functional relevance, and therefore such a set of genes might constitute promising candidates for functional analyses. In this study, we have sequenced the genome of the Pezizomycete Ascodesmis nigricans, and performed comparative transcriptomics of developing fruiting bodies of this fungus, the Pezizomycete Pyronema confluens, and the Sordariomycete Sordaria macrospora With only 27 Mb, the A. nigricans genome is the smallest Pezizomycete genome sequenced to date. Comparative transcriptomics indicated that gene expression patterns in developing fruiting bodies of the three species are more similar to each other than to nonsexual hyphae of the same species. An analysis of 83 genes that are upregulated only during fruiting body development in all three species revealed 23 genes encoding proteins with predicted roles in vesicle transport, the endomembrane system, or transport across membranes, and 13 genes encoding proteins with predicted roles in chromatin organization or the regulation of gene expression. Among four genes chosen for functional analysis by deletion in S. macrospora, three were shown to be involved in fruiting body formation, including two predicted chromatin modifier genes

A polynomial bound for untangling geometric planar graphs

To untangle a geometric graph means to move some of the vertices so that the resulting geometric graph has no crossings. Pach and Tardos [Discrete Comput. Geom., 2002] asked if every n-vertex geometric planar graph can be untangled while keeping at least n^\epsilon vertices fixed. We answer this question in the affirmative with \epsilon=1/4. The previous best known bound was \Omega((\log n / \log\log n)^{1/2}). We also consider untangling geometric trees. It is known that every n-vertex geometric tree can be untangled while keeping at least (n/3)^{1/2} vertices fixed, while the best upper bound was O(n\log n)^{2/3}. We answer a question of Spillner and Wolff [arXiv:0709.0170 2007] by closing this gap for untangling trees. In particular, we show that for infinitely many values of n, there is an n-vertex geometric tree that cannot be untangled while keeping more than 3(n^{1/2}-1) vertices fixed. Moreover, we improve the lower bound to (n/2)^{1/2}.Comment: 14 pages, 7 figure

rac-Carbon­yl{1-[(diphenyl­phosphino)meth­yl]ethanethiol­ato}(triphenyl­phosphine)rhodium(I)

The title compound, [Rh(C15H16PS)(C18H15P)(CO)], was synthesized from the reaction of the ligand rac-[Ph2PCH2CH(CH3)SH] with trans-[Rh(F)(CO)(PPh3)2] in a 1:1 molar ratio in toluene. The Rh atom is four-coordinated in a distorted square-planar geometry with the P—S ligand [Ph2PCH2CH(CH3)S] acting as a chelate and the PPh3 and disordered CO [site occupation factors of 0.61 (5) and 0.39 (5)] ligands completing the coordination