20 research outputs found

    Peanut clump virus and Polymyxa graminis interactions with pearl millet (Pennisetum glaucum [L.] R. Br.) and Sorghum(Sorghum bicolor [L.] Moench)

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    The susceptibility of 6 pearl millet (Ankoutess, ICMV IS 89305, ICMV IS 92222, ICMV IS 94206, Sadoré local and Zatib) and 6 sorghum (Irat 204, Mota Maradi, Nad 1, Sariaso 10, Sepon 82 and Zinder local) accessions to Peanut clump virus and its vector (Polymyxa graminis) was evaluated during the rainy season of 2002 under field conditions in Sadoré, Niger. The frequencies of infection of roots and leaves, analysed by a penicillinase-based DAS-ELISA, confirmed the differences among the pearl millet and sorghum accessions. In pearl millet, the virus was detected in roots and leaves, whereas in sorghum, infection was common in roots but rare in leaves. This was linked with the seed transmission of PCV in millet and not in sorghum. These results confirmed preliminary observations on the movement of the virus within these plant species, underlining the potential role of the viral P15 gene, a suppressor of post-transcriptional gene inactivation. Susceptibility to P. graminis f.sp. tropicalis was assessed under controlled conditions using a zoospore inoculation assay. Infection was quantified by a real-time quantitative PCR assay targeting the ribosomial DNA, and highlighting the differences between plant accessions and species. In addition to guidance on the selection of resistant cultivars, this study also sought to provide a toolbox for improving the understanding of the plant/vector/virus interaction

    Biotechnological approaches for plant viruses resistance: from general to the modern RNA silencing pathway

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    Le Magnificat dans la pensée de Jean-Marc Ela : Perspectives pour une possible mariologie sociale en Afrique

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    Cet article réfléchit sur la possibilité d’une mariologie sociale en Afrique à partir de l’analyse du Magnificat dans la pensée de Jean-Marc Ela. Il part de l’évocation des moments forts de cette analyse pour ensuite mettre en relief trois perspectives porteuses (issues de celle-ci) pouvant aider à penser une telle mariologie. Celle-ci reposerait sur plusieurs axes en l’occurrence la lutte pour la justice sociale, l’engagement pour l’instauration de la paix durable en Afrique et le combat pour la libération des femmes sur ce continent.This article reflects on the possibility of a social mariology in Africa based on the analysis of the Magnificat in Jean-Marc Ela’s thought. It presents the high points of Ela’s interpretation of the Magnificat in order to then emphasize three promising perspectives through which such a mariology can surface. This mariology would be based on several axes, namely the fight for social justice, the commitment to the establishment of lasting peace in Africa and the fight for the liberation of women on this continent

    Detection of Polymyxa graminis in a barley crop in Australia

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    Polymyxa graminis was detected in the roots of barley plants from a field near Wondai, Queensland, in 2009. P. graminis was identified by characteristic sporosori in roots stained with trypan blue. The presence of P. graminis f. sp. tepida (which is hosted by wheat and oats as well as barley) in the roots was confirmed by specific PCR tests based on nuclear ribosomal DNA. P. graminis is the vector of several damaging soil-borne virus diseases of cereals in the genera Furovirus, Bymovirus and Pecluvirus. No virus particles were detected in sap extracts from leaves of stunted barley plants with leaf chlorosis and increased tillering. Further work is required to determine the distribution of P. graminis in Australian grain crops and the potential for establishment and spread of the exotic soil-borne viruses that it vectors
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