Bioactivités, méthodes d’extraction et de purification des polysaccharides de produits naturels

International audienc

The prebiotic potential of a new galactomannan extracted from Astragalus gombo seeds

International audienc

Chemical features and biological activities of water-soluble polysaccharides from Commiphora myrrha gum

International audienc

Partial characterization and phagocytosis activity of water soluble polysaccharides from Plantago ciliata seeds

International audienc

Beneficial Health Potential of Algerian Polysaccharides Extracted from Plantago ciliata Desf. (Septentrional Sahara) Leaves and Seeds

International audienceToday, an ethnobotanical approach makes sense for identifying new active bioactive chemicals from uses of indigenous plants. Two water-soluble enriched polysaccharide fractions (L-PSPN and S-PSPN) were extracted by hot water extraction from the leaves and seeds of Plantago ciliata Desf. (1798), a Mzab indigenous herb currently used in Algeria by traditional healers. Primary investigation was performed for describing the main structural features of these polysaccharides (pectin- and heteroxylan-like compositions) by using colorimetric assays, FTIR spectroscopy, HPAEC/PAD and GC/MS-EI analyses. Some biological activities were also monitored, such as anticomplement, anti-inflammatory (phagocytic ability, NOX2 and MPO inhibitions) and anti-diabetic (α-amylase and α-glucosidase inhibitions). L-PSPC seems able to moderately modulate innate immune system (IC50 around 100 µg/mL) and contribute to wound-healing processes (IC50 close to 217 vs. 443 µg/mL for sodium heparin). S-PSPC shows some potential as an anti-hyperglycemic (IC50 around 4.7 mg/mL) and anti-inflammatory (IC50 ranging from 111 to 203 µg/mL) agent, as well as other (fiber) psyllium-like polysaccharides extracted from Plantago species

Structural characterization and rheological properties of a galactomannan from Astragalus gombo Bunge seeds harvested in Algerian Sahara

International audienceA water soluble polysaccharide (WSP) was extracted and purified from Astragalus gombo seeds (Fabaceae) harvested in Septentrional Sahara (Ouargla, Algeria) with a yield of 6.8% (w/w of the dry seed ground). It was characterized by gas chromatography coupled to the mass spectrometry (GC–MS), size exclusion chromatography with Multi-Angle Light Scattering analysis (SEC-MALLS), high–resolution 1H and 13C NMR, and rheological measurements. The structural characterization indicated that this WSP fraction is a galactomannan with a mannose/galactose ratio of 1.7 formed by a backbone of β-(1,4)-d-mannopyranosyl residues (63%) substituted at O-6 position by a single α-galactopyranose residue (37%). SEC-MALLS analysis revealed that this galactomannan has an average molecular mass (Mw) of 1.1 × 106 g/mol, an intrinsic viscosity of 860 mL/g and, a random coil conformation structure. Rheological analysis in semi diluted regimes shown pseudo-plastic and viscoelastic behaviour

De l’histoire de l’ethnobotanisme en Afrique vers la découverte de nouvelles sources de polysaccharides actifs

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Bioactive polysaccharides from African terrestrial plants: the importance of ethnobotany

International audienc

Bioactive polysaccharides from African terrestrial plants: the importance of ethnobotany

International audienc

Structural characterization and physico-chemical potential of a heteroxylan extracted from Plantago notata Lagasca (Plantaginaceae) seeds

International audienc