903 research outputs found
Technique for Specifying the Fatty Acid at the SN2 Position of Acylglycerol Lipids
A method for specifying a fatty acid at the sn2 position of acylglycerol lipids including (a) transfecting a vector including the SLC1 gene or a variant thereof into embryonic biological material, and (b) allowing the SLC1 gene to specify the type of fatty acid at the sn2 position of acylglycerol lipids. Also provided for is an isolated SLC1 gene and a probe for its detection
Recent Decisions
Comments on recent decisions by Joseph N. Low, Robert A. Stewart, William M. Dickson, Edward G. Coleman, James F. O\u27Rieley, James J. Haranzo, Robert C. Enburg, E. Milton Farley III, Jerome A. Kolenda, Bernard James McGraw, Joseph C. Spalding, R. Emmett Fitzgerald, Joseph T. Helling, John F. Laughlin, Andrew V. Giorgi, and Jack Fena
Comparative biochemical analysis of UHRF proteins reveals molecular mechanisms that uncouple UHRF2 from DNA methylation maintenance
UHRF1 is a histone- and DNA-binding E3 ubiquitin ligase that functions with DNMT1 to maintain mammalian DNA methylation. UHRF1 facilitates DNMT1 recruitment to replicating chromatin through a coordinated mechanism involving histone and DNA recognition and histone ubiquitination. UHRF2 shares structural homology with UHRF1, but surprisingly lacks functional redundancy to facilitate DNA methylation maintenance. Molecular mechanisms uncoupling UHRF2 from DNA methylation maintenance are poorly defined. Through comprehensive and comparative biochemical analysis of recombinant human UHRF1 and UHRF2 reader and writer activities, we reveal conserved modes of histone PTM recognition but divergent DNA binding properties. While UHRF1 and UHRF2 diverge in their affinities toward hemi-methylated DNA, we surprisingly show that both hemi-methylated and hemi-hydroxymethylated DNA oligonucleotides stimulate UHRF2 ubiquitin ligase activity toward histone H3 peptide substrates. This is the first example of an E3 ligase allosterically regulated by DNA hydroxymethylation. However, UHRF2 is not a productive histone E3 ligase toward purified mononucleosomes, suggesting UHRF2 has an intra-domain architecture distinct from UHRF1 that is conformationally constrained when bound to chromatin. Collectively, our studies reveal that uncoupling of UHRF2 from the DNA methylation maintenance program is linked to differences in the molecular readout of chromatin signatures that connect UHRF1 to ubiquitination of histone H3
Detection of hydrogen peroxide with chemiluminescent micelles
The overproduction of hydrogen peroxide is implicated in the progress of numerous life-threatening diseases and there is a great need for the development of contrast agents that can detect hydrogen peroxide in vivo. In this communication, we present a new contrast agent for hydrogen peroxide, termed peroxalate micelles, which detect hydrogen peroxide through chemiluminescence, and have the physical/chemical properties needed for in vivo imaging applications. The peroxalate micelles are composed of amphiphilic peroxalate based copolymers and the fluorescent dye rubrene, they have a ‘stealth’ polyethylene glycol (PEG) corona to evade macrophage phagocytosis, and a diameter of 33 nm to enhance extravasation into permeable tissues. The peroxalate micelles can detect nanomolar concentrations of hydrogen peroxide (>50 nM) and thus have the sensitivity needed to detect physiological concentrations of hydrogen peroxide. We anticipate numerous applications of the peroxalate micelles for in vivo imaging of hydrogen peroxide, given their high sensitivity, small size, and biocompatible PEG corona
Triggered Star Formation in a Massive Galaxy at z=3.8: 4C41.17
Spectropolarimetric observations obtained with the W. M. Keck Telescope of
the z=3.8 radio galaxy 4C41.17 show that the UV continuum emission from this
galaxy, which is aligned with the radio axis, is unpolarized (P[2sigma] <
2.4%). This implies that scattered AGN light, which is generally the dominant
contributor to the rest-frame UV emission in z~1 radio galaxies, is unlikely to
be a major component of the UV flux from 4C41.17. The spectrum shows absorption
lines that are similar to those detected in the spectra of the recently
discovered population of star forming galaxies at z~2-3. A galaxian outflow may
contribute partially to the low ionization absorption lines; however, the high
velocity wings of the high ionization lines are unlikely to be dominated by a
galaxian wind since the implied outflow mass is very large. The detection of
stellar absorption lines, the shape of the SiIV profile, the unpolarized
continuum, the inability of any AGN-related processes to account for the UV
flux, and the similarity of the UV continuum spectra of 4C41.17 and the nearby
starburst region NGC 1741B1 suggest that the UV light in 4C41.17 is dominated
by young stars. If so, the implied star-formation rate is roughly
140-1100Msun/yr. We discuss the possibility that star formation in 4C41.17 was
triggered by the radio source. Our data are consistent with the hypothesis that
4C41.17 is undergoing its major epoch of star formation at z~4, and that by z~1
it will have evolved to have spectral and morphological properties similar to
those observed in known z~1 powerful radio galaxies.Comment: 28 pages (Latex text + figures); Accepted for publication in The
Astrophysical Journal (Dec 1, 1997 issue
Report on an all-sky LIGO search for periodic gravitational waves in the S4 data
We report on an all-sky search with the LIGO detectors for periodic
gravitational waves in the frequency range 50-1000 Hz and having a negative
frequency time derivative with magnitude between zero and Hz/s. Data
from the fourth LIGO science run have been used in this search. Three different
semi-coherent methods of summing strain power were applied. Observing no
evidence for periodic gravitational radiation, we report upper limits on strain
amplitude and interpret these limits to constrain radiation from rotating
neutron stars.Comment: 5 pages, 1 figure, presented at Amaldi7, Sydney (July 2007
Torsion pairs and rigid objects in tubes
We classify the torsion pairs in a tube category and show that they are in
bijection with maximal rigid objects in the extension of the tube category
containing the Pruefer and adic modules. We show that the annulus geometric
model for the tube category can be extended to the larger category and
interpret torsion pairs, maximal rigid objects and the bijection between them
geometrically. We also give a similar geometric description in the case of the
linear orientation of a Dynkin quiver of type A.Comment: 25 pages, 13 figures. Paper shortened. Minor errors correcte
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