Concentrações de ferro, cobre, zinco e manganês em equinos da raça Puro-sangue Lusitano, antes e após exercício

In order to evaluate the sera concentrations of iron, copper, zinc and manganese trace minerals in thirty Pure-bred Lusitano athlete horses before and after exercise certified by clinical and laboratory assessments were studied. Blood samples were taken before and after 20 minutes of a dairy trot and gallop slight training, perfomed in a sandy floor ring, to measure the mineral concentrations, by atomic absorption spectrophotometry. It was observed that after exercising, the serum activity of iron did not change (P=0.2365), whereas copper values have significantly risen after training (P<0.001). On the other hand, zinc and manganese trace minerals decreased after exercise (P<0.001). In conclusion, it is possible to consider that the short time exercise may trigger sweating and consequently to change the seru.Universidade do Oeste Paulista (Unoeste), Rodov. Raposo Tavares Km 572, Bairro Limoeiro, Presidente Prudente, SP 19067-175, Rod. Raposo Tavares Km 572, Bairro Limoeiro. Presidente Prudente, SPDepartamento de Clínica Médica Veterinàra FMVZ, Unesp, Botucatu, SP 18618-970Hospital Veterinário Unoeste, Rodov Raposo Tavares Km 572, Bairro Limoeiro, Presidente Prudente, SPDepartamento de Clínica Médica Veterinàra FMVZ, Unesp, Botucatu, SP 18618-97

Serum activity of creatine kinase and aminotransferase aspartate enzymes of horses submitted to muscle biopsy and incremental jump test

The objective was to evaluate serum activity of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST), which are leakage enzymes responsive to muscle injury, of athletic horses that underwent muscle biopsy and incremental jump test (IJT) involving incremental jumps. The animals were grouped as follows: the first group, horses with history of superior performance (SP); the second, with a history of inferior performance (IP); and lastly, a control group (CG). All groups underwent biopsy of the gluteus medius muscle, while groups SP and IP were also submitted to the incremental jump test (IJT) 24 hours after biopsy. The IJT consisted of three stages with 40 jumps each, where jump height increased progressively, from 40 to 60 and last, 80cm. Blood samples were drawn before biopsy, and 6 and 24 hours after the exercise as well. The levels of CK serum activity increased 6 hours after exercise and decreased 24 hours later in all groups, including CG. AST activity did not increase after biopsy and exercise. There was no increase of both enzyme activities that could be attributed to the exercise, possibly due to exercise short duration and/or low intensity. We conclude that the muscle biopsy was able to show that there was enough stimulus to cause CK enzyme leakage into the plasma, and consequent detection of increased serum activity, while the incremental jump test did not.Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Morfologia e Fisiologia Animal, Jaboticabal, São PauloExército Brasileiro Academia Militar das Agulhas Negras Hospital Veterinário, Rio de JaneiroUniversidade Federal Rural do Rio de Janeiro Instituto de Veterinária Laboratório do Desempenho Equino, Seropédica, Rio de JaneiroUniversidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Departamento de Morfologia e Fisiologia Animal, Jaboticabal, São Paul