Subchronical treatment with Fluoxetine modifies the activity of the MCHergic and hypocretinergic systems. Evidences from peptide CSF concentration and gene expression

In the postero-lateral hypothalamus are located two neuronal systems that utilize the neuropeptides melanin-concentrating hormone (MCH) and hypocretins (also called orexins) as neuromodulators. These systems have reciprocal connections between them, and project throughout the central nervous system. MCH has been involved in the generation of sleep, mainly REM sleep, while hypocretins have a critical role in the generation of wakefulness. MCHergic activity is also involved in the pathophysiology of major depressive disorder (MD). In this regards, intracerebral administration of MCH promotes pro-depressive behaviors (i.e., immobility in the forced swimming test) and REM sleep hypersomnia, which is an important trait of depression. Furthermore, the antagonism of the MCHR-1 receptor has a reliable antidepressant effect, suggesting that MCH is a pro-depressive factor. Hypocretins have been also involved in mood regulation; however, their role in depression is still on debate. Taking these data into account, we explored whether systemic subchronical treatment with Fluoxetine (FLX), a serotonergic antidepressant, modifies the concentration of MCH in the cerebrospinal fluid (CSF), as well as the preproMCH mRNA expression. We also evaluated the hypocretinergic system by quantifying the hypocretin-levels in the CSF and the preprohypocretin mRNA expression. Compared to control, FLX increased the levels of preprohypocretin mRNA without affecting the hypocretin-1 CSF levels. On the contrary, FLX significantly decreased the MCH CSF concentration without affecting the preproMCH gene expression. This result is in agreement with the fact that MCH serum level diminishes during the antidepressant treatment in MD, and supports the hypothesis that an increase in the MCHergic activity could have pro-depressive consequences. (C) 2016 Brazilian Association of Sleep. Production and Hosting by Elsevier B.V.Proyecto de Cooperacion Bilateral Uruguay- Brasil, Dicyt-CNPqPrograma de Desarrollo de Ciencias Basicas (PEDECIBA)Associacao Fundo de Incentivo a Pesquisa (AFIP)Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)Univ Fed Sao Paulo, Dept Psychobiol, Sao Paulo, BrazilUniv Republica, Dept Physiol, Sch Med, Montevideo, UruguayUniv Fed Sao Paulo, Dept Psychobiol, Sao Paulo, BrazilProyecto de Cooperacion Bilateral Uruguay- Brasil, Dicyt-CNPq: ANII-FCE-1-2011-1-5997Web of Scienc

AcMus: an open, integrated platform for room acoustics research

This article describes the design, implementation, and experiences with AcMus, an open and integrated software platform for room acoustics research, which comprises tools for measurement, analysis, and simulation of rooms for music listening and production. Through use of affordable hardware, such as laptops, consumer audio interfaces and microphones, the software allows evaluation of relevant acoustical parameters with stable and consistent results, thus providing valuable information in the diagnosis of acoustical problems, as well as the possibility of simulating modifications in the room through analytical models. The system is open-source and based on a flexible and extensible Java plug-in framework, allowing for cross-platform portability, accessibility and experimentation, thus fostering collaboration of users, developers and researchers in the field of room acoustics.CNPqFAPES

News records of phoresy and hyperphoresy among treefrogs, ostracods, and ciliates in bromeliad of Atlantic forest

Abstract The bromeliad habitat contributes to a high biodiversity and we recorded three treefrog species as new phoretic agents of ostracods, and a first case of hyperphoresy among treefrogs, ostracods and ciliates, in these habitats of Atlantic Forest. Densities of ciliates and ostracods showed significant relationships suggesting the importance of hyperphoresy for ciliate dispersion. Most bromeliad species are able to amplify and sustain the biodiversity due to water storage in a tank formed by their tightly-overlapping leaf bases. Into these tanks, many aquatic organisms live in the water retained (phytotelm) and for them, the bromeliads function as ecological island

The Viscacha survey - II: Structure of star clusters in the Magellanic Clouds periphery

We provide a homogeneous set of structural parameters of 83 star clusters located at the periphery of the Small Magellanic Cloud (SMC) and the Large Magellanic Cloud (LMC). The clusters’ stellar density and surface brightness profiles were built from deep, AO assisted optical images, and uniform analysis techniques. The structural parameters were obtained from King and Elson et al. model fittings. Integrated magnitudes and masses (for a subsample) are also provided. The sample contains mostly low surface brightness clusters with distances between 4.5 and 6.5 kpc and between 1 and 6.5 kpc from the LMC and SMC centres, respectively. We analysed their spatial distribution and structural properties, comparing them with those of inner clusters. Half-light and Jacobi radii were estimated, allowing an evaluation of the Roche volume tidal filling. We found that: (i) for both MCs, the tidal radii are on average larger than those of inner clusters; (ii) the core radii dispersion tends to be greater for LMC clusters located towards the southwest, with position angles of ∼200 degrees and about ∼5 degrees from the LMC centre, i.e., those LMC clusters nearer to the SMC; (iii) the analysis of clusters with ages available revealed that the core radius evolution is similar to the one of inner clusters; (iv) Roche volumes are overfilled for SMC clusters with galactocentric distances closer than 3 kpc.Fil: Santos, Joao F. C.. Universidad de La Serena; Chile. Universidade Federal de Minas Gerais; BrasilFil: Maia, Francisco. Universidade Federal do Rio de Janeiro; BrasilFil: Dias, Bruno. Millennium Institute of Astrophysics; Chile. Universidad Andrés Bello; ChileFil: de O. Kerber, Leandro. Universidade Estadual de Santa Cruz; BrasilFil: Piatti, Andres Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bica, Eduardo. Universidade Federal do Rio Grande do Sul; BrasilFil: Angelo, Mateus S.. Centro Federal de Educacao Tecnológica de Minas Gerais; BrasilFil: Minniti, Dante. Universidad Andrés Bello; Chile. Millennium Institute of Astrophysics; Chile. Vatican Observatory; ItaliaFil: Pérez Villegas, Angeles. Universidade de Sao Paulo; BrasilFil: Roman Lopes, Alexandre. Universidad de La Serena; ChileFil: Westera, Pieter. Universidad Federal Do Abc; BrasilFil: Fraga, Luciano. Laboratorio Nacional de Astrofísica; BrasilFil: Quint, Bruno. Gemini Observatory; ChileFil: Sanmartim, David. Carnegie Institution of Washington; Chil

The VISCACHA survey - I. Overview and first results

TheVISCACHA (VIsible Soar photometry of star Clusters in tApii and Coxi HuguA) Survey is an ongoing project based on deep photometric observations of Magellanic Cloud star clusters, collected using the SOuthern Astrophysical Research (SOAR) telescope together with the SOAR Adaptive Module Imager. Since 2015 more than 200 h of telescope time were used to observe about 130 stellar clusters, most of them with low mass (M < 104MΘ) and/or located in the outermost regions of the Large Magellanic Cloud and the Small Magellanic Cloud.With this high-quality data set, we homogeneously determine physical properties from statistical analysis of colour-magnitude diagrams, radial density profiles, luminosity functions, and mass functions. Ages, metallicities, reddening, distances, present-day masses, mass function slopes, and structural parameters for these clusters are derived and used as a proxy to investigate the interplay between the environment in theMagellanic Clouds and the evolution of such systems. In this first paper we present the VISCACHA Survey and its initial results, concerning the SMC clusters AM3, K37, HW20, and NGC 796 and the LMC ones KMHK228, OHSC3, SL576, SL61, and SL897, chosen to compose a representative subset of our cluster sample. The project's long-term goals and legacy to the community are also addressed.Fil: Maia, Francisco F. S.. Universidade de Sao Paulo; BrasilFil: Dias, Bruno. Universidad Andrés Bello; Chile. European Southern Observatory Santiago; ChileFil: Santos, Joao F. C.. Universidade Federal de Minas Gerais; BrasilFil: De Kerber, Leandro. Universidade de Sao Paulo; BrasilFil: Bica, Eduardo. Universidade Federal do Rio Grande do Sul; BrasilFil: Piatti, Andres Eduardo. Universidad Nacional de Córdoba. Observatorio Astronómico de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barbuy, Beatriz. Universidade de Sao Paulo; BrasilFil: Quint, Bruno. Gemini Observatory; ChileFil: Fraga, Luciano. Laboratório Nacional de Astrofísica; Brasil. Gemini Observatory; ChileFil: Sanmartim, David. Gemini Observatory; ChileFil: Angelo, Mateus S.. Centro Federal de Educação Tecnológica de Minas Gerais; BrasilFil: Hernandez-Jimenez, Jose A.. Universidade de Sao Paulo; BrasilFil: Santrich, Orlando J. Katime. Universidade Estadual de Santa Cruz; BrasilFil: Oliveira, Raphael A. P.. Universidade de Sao Paulo; BrasilFil: Pérez-Villegas, Angeles. Universidade de Sao Paulo; BrasilFil: Souza, Stefano O.. Universidade de Sao Paulo; BrasilFil: Vieira, Rodrigo G.. Universidade de Sao Paulo; BrasilFil: Westera, Pieter. Universidade Federal do ABC; Brasi