21 research outputs found
O papel de alguns micro-organismos na carcinogĂŞnese / The role of some microorganisms in carcinogenesis
A associação de alguns microrganismos envolvidos diretamente com o processo de carcinogĂŞnese vem aumentando a cada dia. A inflamação crĂ´nica causada por esses micrĂłbios, alĂ©m de fatores relacionados ao hospedeiro e ao ambiente, sĂŁo fatores importantes para a iniciação do câncer. Muitos patĂłgenos tĂŞm sido classificados como cancerĂgenos para os seres humanos, tais como: Helicobacter pylori, VĂrus da hepatite B, vĂrus da hepatite C e o papilomavĂrus humano. O estudo teve como objetivo realizar uma revisĂŁo bibliográfica sobre o papel destes micro-organismos na carcinogĂŞnese, assim como estudar os mecanismos utilizados por esses patĂłgenos no processo de geração do câncer. Foi possĂvel observar que alguns fatores de virulĂŞncias presentes nesses microrganismos sĂŁo de fundamental importância no desenvolvimento da doença, tais como a cag A e Vac A da Helicobacter pylori
Promovendo a consciĂŞncia ambiental em uma escola pĂşblica de JoĂŁo Pessoa / Promoting environmental awareness in a public school in JoĂŁo Pessoa
Educação Ambiental é matéria de importante discussão devido ao fato de se compreender a necessidade de uma melhoria do contexto do mundo em que se vive e principalmente no meio escolar. Despreocupação e desinteresse com as questões ambientais são recorrentes entre as pessoas por inúmeros aspectos, porém torna-se essencial discutir a responsabilidade com temas ambientais e as devidas posturas sustentáveis. Neste sentido, este trabalho tem como objetivo principal compreender a percepção dos estudantes a respeito da educação ambiental, assim como a promoção da sensibilização coletiva sobre ideais de sustentabilidade
Diagnóstico pré-natal de doenças genéticas / Pré natal diagnostic of genetic disease
O diagnĂłstico prĂ©-natal de doenças genĂ©ticas envolve exames invasivos, testes de rastreamento e mĂ©todos de triagem, para acompanhamento da viabilidade fetal durante o perĂodo gestacional. Neste artigo de revisĂŁo foram enfatizadas as principais indicações, contra-indicações, complicações, diferenças entre cada um dos procedimentos. Idade materna, perĂodo gestacional, presença de anomalia genĂ©tica na famĂlia sĂŁo os principais prĂ©-requisitos para a escolha do mĂ©todo mais adequado a ser utilizado, sendo de caráter obrigatĂłrio o prĂ©vio conhecimento dos pais a respeito do risco-benefĂcio do mesmo. O aconselhamento genĂ©tico para este tipo de exame diagnĂłstico Ă© de fundamental importância uma vez que o resultado pode mudar completamente as perspectivas de uma famĂlia. Esse tema Ă© rodeado de questionamentos Ă©ticos visto que a vida de um concepto encontra-se em questĂŁo.
Brominated Compounds from Marine Sponges of the Genus Aplysina and a Compilation of Their 13C NMR Spectral Data
Aplysina is the best representative genus of the family Aplysinidae. Halogenated substances are its main class of metabolites. These substances contribute greatly to the chemotaxonomy and characterization of the sponges belonging to this genus. Due to their pharmacological activities, these alkaloids are of special interest. The chemistry of halogenated substances and of the alkaloids has long been extensively studied in terrestrial organisms, while the number of marine organisms studied has just started to increase in the last decades. This review describes 101 halogenated substances from 14 species of Aplysina from different parts of the world. These substances can be divided into the following classes: bromotyramines (A), cavernicolins (B), hydroverongiaquinols (C), bromotyrosineketals (D), bromotyrosine lactone derivatives (E), oxazolidones (F), spiroisoxazolines (G), verongiabenzenoids (H), verongiaquinols (I), and dibromocyclohexadienes (J). A compilation of their 13C NMR data is also part of the review. For this purpose 138 references were consulted
Antifungal Activity of N-(4-Halobenzyl)amides against Candida spp. and Molecular Modeling Studies
Fungal infections remain a high-incidence worldwide health problem that is aggravated by limited therapeutic options and the emergence of drug-resistant strains. Cinnamic and benzoic acid amides have previously shown bioactivity against different species belonging to the Candida genus. Here, 20 cinnamic and benzoic acid amides were synthesized and tested for inhibition of C. krusei ATCC 14243 and C. parapsilosis ATCC 22019. Five compounds inhibited the Candida strains tested, with compound 16 (MIC = 7.8 µg/mL) producing stronger antifungal activity than fluconazole (MIC = 16 µg/mL) against C. krusei ATCC 14243. It was also tested against eight Candida strains, including five clinical strains resistant to fluconazole, and showed an inhibitory effect against all strains tested (MIC = 85.3–341.3 µg/mL). The MIC value against C. krusei ATCC 6258 was 85.3 mcg/mL, while against C. krusei ATCC 14243, it was 10.9 times smaller. This strain had greater sensitivity to the antifungal action of compound 16. The inhibition of C. krusei ATCC 14243 and C. parapsilosis ATCC 22019 was also achieved by compounds 2, 9, 12, 14 and 15. Computational experiments combining target fishing, molecular docking and molecular dynamics simulations were performed to study the potential mechanism of action of compound 16 against C. krusei. From these, a multi-target mechanism of action is proposed for this compound that involves proteins related to critical cellular processes such as the redox balance, kinases-mediated signaling, protein folding and cell wall synthesis. The modeling results might guide future experiments focusing on the wet-lab investigation of the mechanism of action of this series of compounds, as well as on the optimization of their inhibitory potency
Biological Activity of Yangambin on the Postembryonic Development of Chrysomya megacephala
Phaeophytins from Thyrsacanthus ramosissimus Moric. with inhibitory activity on human dna topoisomerase II-α
Our study reports the extraction and isolation of a new phaeophytin derivative 15¹-hydroxy-(15¹-S)-porphyrinolactone, designated anamariaine (1) herein, isolated from the chloroform fraction of aerial parts of Thyrsacanthus ramosissimus Moric. along with the known 15¹-ethoxy-(15¹-S)-porphyrinolactone (2). These compounds were identified by usual spectroscopic methods. Both compounds were subjected to in vitro (inhibitory activity) tests by means of supercoiled DNA relaxation techniques and were shown to display inhibitory activity against human DNA topoisomerase II-α at 50 µM. Interconversion of these two pigments under the mild conditions of the isolation techniques should be highly unlikely but cannot be entirely ruled out