Localization of Minoxidil Sulfotransferase in Rat Liver and the Outer Root Sheath of Anagen Pelage and Vibrissa Follicles

The precise biochemical mechanism and site(s) of action by which minoxidil stimulates hair growth are not yet clear. Minoxidil sulfate is the active metabolite of minoxidil, with regard to smooth muscle vasodilation and hair growth. Formation of minoxidil sulfate is catalyzed by specific PAPS-dependent sulfotransferase(s) and minoxidil-sulfating activities have been previously reported to be present in liver and hair follicles. One of these minoxidil-sulfating enzymes has been purified from rat liver (rat minoxidil sulfotransferase, MST) and a rabbit anti-MST antibody has been prepared. Using this anti-MST antibody, we have immunohistochernically localized minoxidil sulfotransferase in the liver and anagen hair follicles from rat. In rat pelage and vibrissa follicles, this enzyme is localized within the cytoplasm of epithelial cells in the lower outer root sheath. Although the immunolocalization of MST might not necessarily correlate with the MST activity known to be present in anagen follicles, the results of this study strongly suggest that the lower outer root sheath of the hair follicle may serve as a site for the sulfation of topically applied minoxidil

FORMULASI TEPUNG KECAMBAH KEDELAI DAN TEPUNG IKAN TUNA SEBAGAI BAHAN MP-ASI BUBUK INSTAN UNTUK BAYI USIA 6 - 8 BULAN

Makanan Pendamping Air Susu Ibu (MP-ASI) adalah makanan atau minuman yang diberikan pada bayi atau anak usia 6-24 bulan guna memenuhi kebutuhan gizi selain dari Air Susu Ibu. Pada umur 6-8 bulan asupan energi dari ASI yaitu 437 Kal/hari, sedangkan yang dianjurkan 783 Kal, jadi energi yang dibutuhkan dari MP-ASI yaitu 346 Kal/hari. Salah satu upaya yang diharapkan dapat membantu meningkatkan mutu gizi yang ada dalam MP-ASI adalah dengan menggunakan tepung kecambah kedelai dan tepung ikan tuna.Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) menggunakan faktor tunggal yaitu formulasi tepung kecambah kedelai dan tepung ikan tuna (F) yang terdiri dari delapan perlakuan yaitu F1 (15%:85%), F2 (25%:75%), F3 (35%:65%), F4 (45%:55%), F5 (55%:45%, F6 (65%:35%), F7 (75%:25%), dan F8 (85%:15%).Hasil penelitian menunjukkan bahwa semakin besar proporsi tepung kecambah kedelai maka kadar abu, lemak dan nilai energi semakin meningkat, sedangkan semakin tinggi proporsi tepung ikan tuna maka kadar protein dalam formula MP-ASI juga semakin meningkat secara signifikan. Berdasarkan hasil analisa kandungan gizi dan nilai energi dapat disimpulkan bahwa MP-ASI Bubuk Instan dengan proporsi F1 (15%:85%), F2 (25%:75%), F3 (35%:65%), F4 (45%:55%), F5 (55%:45%) dan F6 (65%:35%) merupakan MP-ASI Bubuk Instan yang memenuhi persyaratan MP-ASI diantara delapan formulasi yang ada

In-situ surface technique analyses and ex-situ characterization of Si1-xGex epilayers grown on Si(001)-2 ×1 by molecular beam epitaxy

Si1-xGex epilayers grown by Molecular Beam Epitaxy on Si(001) at 400 ○C have been analyzed in-situ by surface techniques such as X-ray and Ultraviolet Photoelectron Spectroscopies (XPS and UPS), Low Energy Electron Diffraction (LEED) and photoelectron diffraction (XPD). The Ge surface concentrations (x) obtained from the ratios of Ge and Si core level intensities are systematically higher than those obtained by the respective evaporation fluxes. This indicates a Ge enrichment in the first overlayers confirmed by Ge-like UPS valence band spectra. The structured crystallographic character of the epilayers is ascertained by LEED and XPD polar scans in the (100) plane since the Ge Auger LMM and the Si 2p XPD intensity patterns from the Si1-xGex epilayers are identical to those of the Si substrate. The residual stress in the epilayer is determined by ex-situ X-ray diffraction (XRD) which also allows, as Rutherford Back Scattering (RBS), Ge concentration determinations

Ubiquitination and sumoylation of the HTLV-2 Tax-2B protein regulate its NF-κB activity: a comparative study with the HTLV-1 Tax-1 protein.

BACKGROUND:Retroviruses HTLV-1 and HTLV-2 have homologous genomic structures but differ significantly in pathogenicity. HTLV-1 is associated with Adult T cell Leukemia (ATL), whereas infection by HTLV-2 has no association with neoplasia. Transformation of T lymphocytes by HTLV-1 is linked to the capacity of its oncoprotein Tax-1 to alter cell survival and cell cycle control mechanisms. Among these functions, Tax-1-mediated activation of cellular gene expression via the NF-κB pathway depends on Tax-1 post-translational modifications by ubiquitination and sumoylation. The Tax-2 protein of HTLV-2B (Tax-2B) is also modified by ubiquitination and sumoylation and activates the NF-κB pathway to a level similar to that of Tax-1. The present study aims to understand whether ubiquitination and sumoylation modifications are involved in Tax-2B-mediated activation of the NF-κB pathway.RESULTS:The comparison of Tax-1 and Tax-2B lysine to arginine substitution mutants revealed conserved patterns and levels of ubiquitination with notable difference in the lysine usage for sumoylation. Neither Tax-1 nor Tax-2B ubiquitination and sumoylation deficient mutants could activate the NF-κB pathway and fusion of ubiquitin or SUMO-1 to the C-terminus of the ubiquitination and sumoylation deficient Tax-2B mutant strikingly restored transcriptional activity. In addition, ubiquitinated forms of Tax-2B colocalized with RelA and IKKγ in prominent cytoplasmic structures associated with the Golgi apparatus, whereas colocalization of Tax-2B with the RelA subunit of NF-κB and the transcriptional coactivator p300 in punctate nuclear structures was dependent on Tax-2B sumoylation, as previously observed for Tax-1.CONCLUSIONS:Both Tax-1 and Tax-2 activate the NF-κB pathway via similar mechanisms involving ubiquitination and sumoylation. Therefore, the different transforming potential of HTLV-1 and HTLV-2 is unlikely to be related to different modes of activation of the canonical NF-κB pathway

Tuning electrical properties of hierarchically assembled Al-doped ZnO nanoforests by room temperature Pulsed Laser Deposition

Large surface area, 3D structured transparent electrodes with effective light management capability may represent a key component in the development of new generation optoelectronic and energy harvesting devices. We present an approach to obtain forest-like nanoporous/hierarchical Al-doped ZnO conducting layers with tunable transparency and light scattering properties, by means of room temperature Pulsed Laser Deposition in a mixed Ar:O2 atmosphere. The composition of the background atmosphere during deposition can be varied to modify stoichiometry-related defects, and therefore achieve control of electrical and optical properties, while the total background pressure controls the material morphology at the nano- and mesoscale and thus the light scattering properties. This approach allows to tune electrical resistivity over a very wide range (10^-1 - 10^6 Ohm*cm), both in the in-plane and cross-plane directions. Optical transparency and haze can also be tuned by varying the stoichiometry and thickness of the nano-forests

Determining Material Response for Polyvinyl Butyral (PVB) in Blast Loading Situations

Protecting structures from the effect of blast loads requires the careful design of all building components. In this context, the mechanical properties of Polyvinyl Butyral (PVB) are of interest to designers as the membrane behaviour will affect the performance of laminated glass glazing when loaded by explosion pressure waves. This polymer behaves in a complex manner and is difficult to model over the wide range of strain rates relevant to blast analysis. In this study, data from experimental tests conducted at strain rates from 0.01 s−1 to 400 s−1 were used to develop material models accounting for the rate dependency of the material. Firstly, two models were derived assuming Prony series formulations. A reduced polynomial spring and a spring derived from the model proposed by Hoo Fatt and Ouyang were used. Two fits were produced for each of these models, one for low rate cases, up to 8 s−1, and one for high rate cases, from 20 s−1. Afterwards, a single model representing all rates was produced using a finite deformation viscoelastic model. This assumed two hyperelastic springs in parallel, one of which was in series with a non-linear damper. The results were compared with the experimental results, assessing the quality of the fits in the strain range of interest for blast loading situations. This should provide designers with the information to choose between the available models depending on their design needs

Enhanced follicular delivery of finasteride to human scalp skin using heat and chemical penetration enhancers

© The Author(s) 2020. This article is an open access publication. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.Purpose The aim of this work was to evaluate whether improved topical delivery of finasteride, focussed to the hair follicles of human scalp skin could be achieved with application of short durations of heat and use of specific chemical penetration enhancers. Methods Franz cell experiments with human scalp skin were performed with a range of chemical penetration enhancers at 32°C and 45°C to simulate normal and heated conditions. Selected chemical penetration enhancers were taken forward for finite dose Franz cell studies which examined the effect of heat produced by a prototype external heating system that supplied either 20 or 30 min of additional heat over both a 24 h and a 1 h time period. Results Short durations of externally applied heat significantly increased finasteride penetration into human scalp skin after 24 h. Analysis of drug distribution in the skin after 1 h and 24 h indicated that both heat and chemical penetration enhancer selection influenced drug delivery to the hair follicles. Conclusion The use of short durations of heat in combination with specific chemical penetration enhancers was able to increase the delivery of finasteride to human scalp skin and provide focussed drug delivery to the hair follicles.Peer reviewe

A small-N cross-sectional study of British unions' environmental attitudes and activism - and the prospect of a green-led renewal

Unions understand the environmental agenda as a technocentric one but also believe it can function as a vehicle for renewal. It is developing slowly, with unions behaving cautiously—resources are scarce. Although popular with members, there is limited evidence that it is effective as a recruitment tool and whilst employers are willing to work in partnership with unions on it, this may confer only phony insider status. Overall, the agenda has limited appeal to the types of employees and employers unions must recruit in order to grow. Identifying a clear environmental premium for members may help

Democracy, protest and public sphere in Russia after the 2011–2012 anti-government protests: digital media at stake

The 2011–2012 Russian protest mobilisations were largely enabled by the rise of social networks. Social and technological advancements paired to pave the way for the ‘biggest protests since the fall of USSR’. Ubiquitous and uncensored social media facilitated the networking and mobilisation for this protest activity: Liberal masses were able to share and discuss their grievances, unite and coordinate online for the offline protest. The digitally savvy protest public developed to confront the government, which appeared to be astonished by the scale of protest. Those mobilisations marked an important gap between the government’s conception of the society and the real state of resistance. This article studies three main hypotheses regarding the potential of the protest movement in Russia. The hypotheses were drawn from recent sociological, political and media studies on Russian resistance. Current research aims to contribute to the debate from the digital media perspective. It therefore evaluates three main assumptions: Digital media have the potential to empower, dependent upon the relevant political, social and economic factors; digital media isolates protest publics and therefore may be more useful for the government than the resistance; and recent censorship of digital media communication signals a tightening of both formal and informal restrictions against opposition and protest politics. This article uses theoretical and factual evidence on the limitations of democracy and the public sphere and conceptualises the government’s management of resistance in Russia during and after the 2011–2012 protests. It studies how the hybrid political regime in Russia balances restrictions on freedom of speech with strengthened state propaganda and how it mediates media oppression and invites self-censorship. Finally, it examines how the state communication watchdog has recently focused its attention at the digital realm. This move confirms the importance of the online protest communication for the Russian political environment. Yet the state’s acknowledgement of digital political resistance may lead to further oppression and curbing of this emerging component of Russian politics