Verifikasi Metode Penetapan Kadar Fenol Total Cara Folin-Ciocalteu

The Biofarmaka Laboratory is currently preparing for ISO/IEC 17025 accreditation, which requires the use of validated analytical methods and verified procedures for standard methods. This study aims to support the verification of a method for determining total phenolic content using the Folin–Ciocalteu reagent, as outlined in the Indonesian Herbal Pharmacopoeia, First Edition (2013). Verification was conducted by evaluating linearity, precision, accuracy, limit of detection (LoD), and limit of quantification (LoQ). All parameters met the acceptance criteria based on ICH Q2(R1) and ISO/IEC 17025:2017 guidelines, with a determination coefficient (r²) of 0.9993, precision (%RSD) of 1.67%, and accuracy (% recovery) of 101.40%. The LoD and LoQ values were 7.06 ppm and 21.40 ppm, respectively, indicating the method's sensitivity in detecting and quantifying low concentrations of total phenolics. Although the analysis was conducted on green tea extract (Camellia sinensis), the method is generally applicable to various other plant extracts. These results demonstrate that the method is suitable for routine laboratory use and reliable for implementation in support of ISO/IEC 17025-based quality systems

Rancang Bangun Alat Fermentor Sangat Sederhana (FSS) Pada Perbanyakan Agensia Hayati Media Cair Sebagai Pengendali Organisme Pengganggu Tanaman (OPT)

The practicum of liquid media biological agent multiplication at the Jember State Polytechnic Plant Protection Laboratory still experiences obstacles, especially the FSS installation, which often results in contamination. The main cause is the limitations of the very simple fermenter (FSS) tool, so an effective tool design solution is needed. This research was conducted with the following objectives: (1) Designing the FSS tool for the multiplication of liquid media biological agents, (2) Developing Standard Operating Procedures (SOPs) for the FSS tool for the multiplication of liquid media biological agents and (3) Testing the FSS tool for the multiplication of liquid media biological agents. The research was conducted at the Jember State Polytechnic Plant Protection Laboratory in May - November 2024 with funding from the Jember State Polytechnic PNBP. This research uses a descriptive method, visual observation of the FSS tool installation for the multiplication of several types of liquid media biological agents, observation of the success rate of uncontaminated ECG liquid media, and then the data will be displayed in the form of a graphic diagram. The results of the study indicate that: 1. The design of the FSS installation tool can be used for the multiplication of liquid agents, 2. Standard Operating Procedures (SOP) can be used as a guideline for the multiplication of liquid media biological agents, 3. The test results of the FSS installation tool on the multiplication of several liquid media biological agents showed a success rate of Beauverria bassiana 75%, Trichoderma sp 91.67%, Metarhizium sp. 83.33% and Paenibacillus 83.33%. The constraints are that the propagation room is less sterile and the inoculation process is still separate from the installation tool. The benefits of the FSS tool design on the multiplication of this biological agent can be recommended as a tool for practical activities in the multiplication of liquid media biological agents in the Laboratory of Plant Protection, Research and Community Service

The Effect Of Shoot Growth From Single Stick Rejuvination With Clipping Treatment On Branch Growth Of Robusta Coffee (Coffea Canephora L.) BP308 Clone

Robusta coffee (Coffea canephora L.) is the type of coffee most widely developed by farmers in Indonesia. The BP308 is a clone discovered by the coffee and cocoa research center which is included in the Robusta coffee clone which has a large and sturdy stature. One of the coffee plant maintenance activities that needs to be carried out is pruning so that coffee plant productivity can be optimal by removing old branches that are less productive and replacing them with young branches that are more productive. Pruning is a clipping, especially for plants that have difficulty growing reproductive branches. Clipping is carried out at the beginning of the rainy season so as not to stimulate the formation of flowers on the circumcised branches. The method used T-test calculations from the results of rejuvenation of single stems with treatment of one node and two nodes resulting from clipping on shoot growth and the data is then analyzed using a quantitative descriptive analysis method. The results show that there is no influence on shoot length parameters. However, it has a significant increase in the number of leaves from 1,47 to 2,33, and it has a significant increase in shoot diameter from 1,63 mm to 2,38 mm. This is the first study comparing single vs. double node clipping on BP308 clone under specific microclimate conditions

Evaluation of feed digestibility in goats based on palm oil industry waste and fisheries in Southwest Papua

Palm oil and fishery waste are recognized as potential environmental concerns, necessitating their processing for reuse as feed material. This study aimed to evaluate the digestibility of feed materials based on palm oil waste and fish waste, utilizing EM4 and Aspergillus niger in goats. Six male goats was randomly used to assess the digestibility of dry matter (DM) and crude protein (CP) in six types of feed, each featuring distinct waste and fermenter, employing a 6x6 Latin Square Design. Digestibility data were obtained through in vitro and in vivo methods. The in vivo method showedthat T2, made from palm oil waste and Aspergillus niger, demonstrated the highest DM digestibility of 62.10±7.64%. Conversely, the highest CP digestibility was found in T4, which consists of fish waste with Aspergillus niger, yielding an average protein digestibility value of 67.21±9.81%. In contrast, the in vitro method indicated that the highest DM digestibility occurred in T6, which combines palm and fish waste with Aspergillus niger, averaging 65.25±5.59%. Meanwhile, T4 (fish waste with Aspergillus niger) showed a protein digestibility of 71.69±8.7%. These findings suggest that palm oil waste and fish waste can be tailored to meet livestock nutritional requirements. Furthermore, they indicate that utilizing Aspergillus niger is a more cost-effective approach compared to EM4

Perbandingan Pengolahan Limbah Urin Sapi sebagai Pupuk Organik Cair (POC) dengan Metode Fermentasi Aerob dan Anaerob

Urin sapi merupakan salah satu limbah peternakan yang diproduksi dalam jumlah banyak setiap harinya. Hal ini tentu mempengaruhi kualitas lingkungan sekitar industri peternakan jika tidak ditangani dengan baik. Limbah yang dihasilkan TEFA Sapi Perah Politeknik Negeri Jember juga sangat memerlukan tindakan pengolahan limbah untuk menangani limbah yang dihasilkan oleh ternak sapi perah setiap harinya.  Urin sapi dapat dimanfaatkan dengan cara diolah menjadi pupuk organik cair setelah dicampur dengan bahan tertentu. Pupuk organik cair (POC) merupakan salah satu teknologi inovasi dalam penanganan limbah urin.  Pembuatan pupuk organik cair dapat menggunakan metode fermentasi aerob dan anaerob. Penelitian ini diharapkan dapat memberikan informasi mengenai efektifitas dari metode tersebut dalam pembuatan pupuk organik cair urin sapi. Selain itu penelitian ini diharapkan dapat menjadi titik awal untuk pembuatan produk pupuk organik cair yang dapat diproduksi secara terbatas oleh Laboratorium Teknologi Pakan. Penelitian ini menggunakan 2 perlakuan yaitu metode fermentasi aerob dan anaerob. Pembuatan Pupuk Organik Cair Urine Sapi yang dilakukan baik menggunakan metode fermentasi aerob dan aerob tidak memenuhi standar dari Kementan (2019). Total NPK pada kedua metode tidak memenuhi standar (2-6%) dan pada metode aerob memiliki total bakteri Salmonella yang lebih dari standar (1x102 CFU/g)

Excellence Service Strategy through Laboratory System Development in Health Department

The Health Department has nineteen laboratories that serve various important purposes, including practicums and learning, supporting clinical research, clinical case simulations, research and system development, professional training, and health services. Despite their significance, these laboratories face challenges in presenting their profiles effectively to the public and stakeholders. This research aims to develop an Information System designed specifically for these laboratories to improve visibility and access to information regarding their services, technology, achievements, and advantages. The research follows a prototyping method to develop the system, which is chosen due to its flexibility, the complexity of the laboratories' needs, and the iterative nature of system development. The research was conducted over a period of six months, and the main steps involved included: early prototype development, rapid design and system testing. The web-based Laboratory Information System has been successfully developed and deployed, effectively addressing the challenges of information presentation, and supporting the growth and development of the laboratories

POTENSI BAKTERIOFAG SEBAGAI BIOKONTROL KASUS RESISTENSI ANTIBIOTIK PADA BUDIDAYA UDANG DI INDONESIA: POTENTIAL OF BACTERIOPHAGES AS BIOCONTROL OF ANTIBIOTIC RESISTANCE CASES IN SHRIMP CULTIVATION IN INDONESIA

Indonesia merupakan negara kepulauan yang memiliki kekayaan hayati laut, sehingga memiliki potensi dalam bidang kelautan dan perikanan berkelanjutan. Di  Indonesia  budidaya  udang  telah berkembang  sangat  pesat  dan memiliki peminat yang luas baik di pasar nasional maupun internasional. Namun, terdapat satu permasalahan yang sering terjadi pada bidang akuakultur khususnya pada budidaya udang. Kegagalan produksi panen pada komoditas udang banyak disebabkan oleh bakteri pathogen khususnya jenis Vibrio spp. Salah satu cara yang sering diterapkan dalam mencegah ataupun mengobati infeksi bakteri yaitu dengan penggunaan antibiotik. Penggunaan antibiotic yang berlebihan memiliki dampat yang cukup serius terhadap bidang budidaya udang serta lingkungan sekitarnya. Antibiotik dapat menyebabkan Multiple Antibiotic Resistance yang juga dapat membahayakan manusia. Berdasarkan hal tersebut, maka dibutuhkan alternatif lain yang lebih ramah lingkungan sebagai pengganti antibiotik. Bakteriofag dapat menjadi salah satu alternatif yang menjanjikan dalam mencegah/mengontrol infeksi yang disebabkan oleh bakteri patogen. Bakteriofag merupakan virus yang memiliki sifat spesifik terhadap bakteri inang sehingga tidak akan membahayakan lingkungan ataupun manusia. Penulisan tinjauan ini bertujuan untuk merangkum beberapa potensi yang menjanjikan terkait penerapan bakteriofag pada bidang budidaya udang khususnya di Indonesia

The Production Of Bluder Substitute Made Of Psyllium Husk Flour As Fiber-Rich Food To Prevent Constipation

Continuous constipation can cause several diseases such as hemorrhoids, colon cancer, diverticular disease. It is generally characterized by stool which becomes increasingly harder and larger. As a result, this causes lower frequency of defecation. Consuming highly fibrous food sources is one decent measure to address constipation. One ingredient high in fiber is psyllium husk. This study aims to determine the fiber content in bluder made of psyllium husk flour as a bread substitute to prevent constipation. It employed Completely Randomized Design (CRD) with 5 treatments (flour and psyllium husk flour), inter alia T1 (87.6% and 12.4%), T2 (86.4% and 13.6%), T3 (85.2% and 14.2%), T4 (84% and 16%), and T5 (82.2% and 17.2% flour), each of which was repeated 5 times. The results showed that T5 has the highest fiber content at 9.85 grams/60 grams. The addition of psyllium husk flour had a significant effect on fiber content and the physical properties of bluder, while the best treatment in this study was T3 (85.2% flour and 14.8% psyllium husk flour). One loaf of bluder weighing 65 grams provides energy content of 228 kcal, 9 grams of protein, 8 grams of fat, 30 grams of carbohydrates, and 4.8 grams of fiber

Perancangan Sistem Kontrol Alat Ukur Viscositas Pada Fluida Menggunakan Arduino Sebagai Penunjang Praktikum Di Laboratorium Teknik Tata Air

Salah satu sifat cairan yang menentukan besarnya perlawanan terhadap gaya gesek adalah viskositasnya, yang juga dikenal sebagai ukuran kekentalan fluida, yang menunjukkan besar kecilnya gesekan di dalam fluida. Semakin tinggi viskositas fluida, semakin sulit suatu benda bergerak di dalamnya. Viskositas fluida ditentukan oleh gesekan molekul-molekul fluida yang menghalangi aliran fluida. Jenis fluida yang mudah mengalir memiliki nilai viskositas yang rendah, dan sebaliknya. Ini adalah gesekan yang menghalangi aliran zat cair. Di laboraturium teknik tata air, pengukuran viskositas fluida sederhana biasanya dilakukan dengan menggunakan teknik bola jatuh. Metode ini digunakan untuk memasukkan bola ke dalam tabung yang berisi fluida, seperti pelumas atau oli, dan kemudian menghitung waktu tempuh dengan menggunakan hukum stokes. Praktikum fisik di laboratorium teknik tata air. Desain sistem kontrol alat ukur viscositas fluida dengan arduino alat yang dirancang terdiri dari komponen yang dapat mengukur kekentalan fluida. Jika sensor infra red digunakan, diharapkan mereka dapat menjawab masalah alami. Selain itu, diperlukan pengendali seperti mikrokontroler yang dapat mengontrol sensor secara otomatis

Respon Hasil Produksi Kacang Tanah (Arachis hypogea) terhadap Aplikasi PGPR dan Rhizobium

Increasing peanut production can be done by applying rhizobium and PGPR. This study aims to examine the effect of PGPR and Rhizobium on peanuts. The research was conducted from December 2022 to February 2023 in Patemon Village, Pakusari District, Jember Regency. Experimental design using factorial RAK. The first factor was the concentration of PGPR consisting of 10.5 ml/l, 12.5 ml/l and 14.5 ml/l. The second factor was the dose of Rhizobium inoculants consisting of 25 kg/ha, 50 kg/ha, and 75 kg/ha. The results of this study indicated that there was no real interaction between the PGPR and Rizobum treatments. As a single factor, PGPR with a concentration of 12.5 ml/l gave an effect on the variable number of ginophores (72.1). While the application of Rhizobium inoculant at a dose of 75 kg/ha showed the best results on the number of pods/sample (30.90), weight of pods/sample (62.12 g), weight of seeds/sample (44.74 g), and weight of seeds/plot (850.60 gr). . It is suspected that the application of PGPR to peanuts and inoculation of Rhizobium plays a role in increasing nutrient uptake which in turn increases growth and production.    Peningkatan produksi kacang tanah dapat diakukan dengan aplikasi rhizobium dan PGPR. Penelitian ini bertujuan untuk mengkaji pengaruh pemberian PGPR dan Rizobium pada kacang tanah. Penelitian dilaksanakan pada bulan Desember 2022 hingga Februari 2023 di Desa Patemon, Kecamatan Pakusari, Kabupaten Jember. Rancangan Percobaan menggunakan RAK faktorial. Faktor pertama yaitu konsentrasi PGPR terdiri dari 10,5 ml/l, 12,5 ml/l, dan 14,5 ml/l. Faktor kedua yaitu dosis inokulan Rizobium terdiri dari 25 kg/ha, 50 kg/ha, dan 75 kg/ha. Hasil penelitian ini menunjukkan tidak adanya interaksi nyata antar perlakuan PGPR dan Rizobum. Secara faktor tunggal, PGPR dengan konsentrasi 12,5 ml/l memberikan pengaruh pada variabel jumlah ginofor (72,1). Sedangkan aplikasi inokulan Rizobium dengan dosis 75 kg/ha secara nyata menunjukan hasil terbaik pada jumlah polong/sampel (30,90), berat polong/sampel (62,12 g), berat biji/sampel (44,74 g), dan berat biji/plot (850,60 g). . Diduga pengaplikasian PGPR pada kacang tanah dan penginokulasian Rizobium berperan pada peningkatan serapan hara yang kemudian meningkatkan pertumbuhan dan produksi.   &nbsp