Impact of Resonant Magnetic Perturbations on Zonal Modes, Drift-Wave Turbulence and the L-H Transition Threshold

We study the effects of Resonant Magnetic Perturbations (RMPs) on turbulence, flows and confinement in the framework of resistive drift-wave turbulence. This work was motivated, in parts, by experiments reported at the IAEA 2010 conference [Y. Xu {\it et al}, Nucl. Fusion \textbf{51}, 062030] which showed a decrease of long-range correlations during the application of RMPs. We derive and apply a zero-dimensional predator-prey model coupling the Drift-Wave Zonal Mode system [M. Leconte and P.H. Diamond, Phys. Plasmas \textbf{19}, 055903] to the evolution of mean quantities. This model has both density gradient drive and RMP amplitude as control parameters and predicts a novel type of transport bifurcation in the presence of RMPs. This model allows a description of the full L-H transition evolution with RMPs, including the mean sheared flow evolution. The key results are: i) The L-I and I-H power thresholds \emph{both} increase with RMP amplitude |\bx|, the relative increase of the L-I threshold scales as \Delta P_{\rm LI} \propto |\bx|^2 \nu_*^{-2} \gyro^{-2}, where $\nu_*$ is edge collisionality and \gyro is the sound gyroradius. ii) RMPs are predicted to \emph{decrease} the hysteresis between the forward and back-transition. iii) Taking into account the mean density evolution, the density profile - sustained by the particle source - has an increased turbulent diffusion compared with the reference case without RMPs which provides one possible explanation for the \emph{density pump-out} effect.Comment: 30 pages, IAEA-based articl

Enzyme microarrays assembled by acoustic dispensing technology

Miniaturizing bioassays to the nanoliter scale for high-throughput screening reduces the consumption of reagents that are expensive or difficult to handle. Through the use of acoustic dispensing technology, nanodroplets containing 10 μM ATP (3 μCi/μL 32P) and reaction buffer in 10% glycerol were positionally dispensed to the surface of glass slides to form 40-nL compartments (100 droplets/slide) for Pim1 (proviral integration site 1) kinase reactions. The reactions were activated by dispensing 4 nL of various levels of a pyridocarbazolo-cyclopentadienyl ruthenium complex Pim1 inhibitor, followed by dispensing 4 nL of a Pim1 kinase and peptide substrate solution to achieve final concentrations of 150 nM enzyme and 10 μM substrate. The microarray was incubated at 30 °C (97% Rh) for 1.5 h. The spots were then blotted to phosphocellulose membranes to capture phosphorylated substrate. With phosphor imaging to quantify the washed membranes, the assay showed that, for doses of inhibitor from 0.75 to 3 μM, Pim1 was increasingly inhibited. Signal-to-background ratios were as high as 165, and average coefficients of variation for the assay were not, vert, ∼20%. Coefficients of variation for dispensing typical working buffers were under 5%. Thus, microarrays assembled by acoustic dispensing are promising as cost-effective tools that can be used in protein assay development

VLBI study of water maser emission in the Seyfert 2 galaxy NGC5793. I: Imaging blueshifted emission and the parsec-scale jet

We present the first result of VLBI observations of the blueshifted water maser emission from the type 2 Seyfert galaxy NGC5793, which we combine with new and previous VLBI observations of continuum emission at 1.7, 5.0, 8.4, 15, and 22 GHz. Maser emission was detected earlier in single-dish observations and found to have both red- and blueshifted features relative to the systemic velocity. We could image only the blueshifted emission, which is located 3.6 pc southwest of the 22 GHz continuum peak. The blueshifted emission was found to originate in two clusters that are separated by 0.7 milliarcsecond (0.16 pc). No compact continuum emission was found within 3.6 pc of the maser spot. A compact continuum source showing a marginally inverted spectrum between 1.7 and 5.0 GHz was found 4.2 pc southwest of the maser position. The spectral turnover might be due to synchrotron self-absorption caused by a shock in the jet owing to collision with dense gas, or it might be due to free-free absorption in an ionized screen possibly the inner part of a disk, foreground to the jet. The water maser may be part of a maser disk. If so, it would be rotating in the opposite sense to the highly inclined galactic disk observed in CO emission. We estimate a binding mass within 1 pc of the presumed nucleus to be on the order of 10^7 Msun. Alternatively, the maser emission could result from the amplification of a radio jet by foreground circumnuclear molecular gas. In this case, the high blueshift of the maser emission might mean that the masing region is moving outward away from the molecular gas surrounding an active nucleus.Comment: 20 pages, 6 figures, to appear in ApJ, Oct. 200

Hemodynamic Regulation of Inflammation at the Endothelial-Neutrophil Interface

Arterial shear stress can regulate endothelial phenotype. The potential for anti-inflammatory effects of shear stress on TNFα-activated endothelium was tested in assays of cytokine expression and neutrophil adhesion. In cultured human aortic endothelial cells (HAEC), arterial shear stress of 10 dyne/cm2 blocked by \u3e 80% the induction by 5 ng/ml TNFα of interleukin-8 (IL-8) and IL-6 secretion (50% and 90% reduction, respectively, in the presence of nitric oxide synthase antagonism with 200 μM nitro-L-arginine methylester, L-NAME). Exposure of TNFα-stimulated HAEC to arterial shear stress for 5 hr also reduced by 60% (P &#; 0.001) the conversion of neutrophil rolling to firm arrest in a venous flow assay conducted at 1 dyne/cm2. Also, neutrophil rolling lengths at 1 dyne/cm2 were longer when TNFα-stimulated HAEC were presheared for 5 hr at arterial stresses. In experiments with a synthetic promoter that provides luciferase induction to detect cis interactions of glucocorticoid receptor (GR) and NFκB, shear stress caused a marked 40-fold induction of luciferase in TNFα-treated cells, suggesting a role for GR pathways in the anti-inflammatory actions of fluid shear stress. Hemodynamic force exerts anti-inflammatory effects on cytokine activated endothelium by attenuation of cytokine expression and neutrophil firm arrest

Sensory Prioritization in Rats: Behavioral Performance and Neuronal Correlates

Operating with some finite quantity of processing resources, an animal would benefit from prioritizing the sensory modality expected to provide key information in a particular context. The present study investigated whether rats dedicate attentional resources to the sensory modality in which a near-threshold event is more likely to occur. We manipulated attention by controlling the likelihood with which a stimulus was presented from one of two modalities. In a whisker session, 80% of trials contained a brief vibration stimulus applied to whiskers and the remaining 20% of trials contained a brief change of luminance. These likelihoods were reversed in a visual session. When a stimulus was presented in the high-likelihood context, detection performance increased and was faster compared with the same stimulus presented in the low-likelihood context. Sensory prioritization was also reflected in neuronal activity in the vibrissal area of primary somatosensory cortex: single units responded differentially to the whisker vibration stimulus when presented with higher probability compared with lower probability. Neuronal activity in the vibrissal cortex displayed signatures of multiplicative gain control and enhanced response to vibration stimuli during the whisker session. In conclusion, rats allocate priority to the more likely stimulus modality and the primary sensory cortex may participate in the redistribution of resources

Edge Shear Flows and Particle Transport near the Density Limit in the HL-2A Tokamak

Edge shear flow and its effect on regulating turbulent transport have long been suspected to play an important role in plasmas operating near the Greenwald density limit $n_G$. In this study, equilibrium profiles as well as the turbulent particle flux and Reynolds stress across the separatrix in the HL-2A tokamak are examined as $n_G$ is approached in ohmic L-mode discharges. As the normalized line-averaged density $\bar{n}_e/n_G$ is raised, the shearing rate of the mean poloidal flow $\omega_{\rm sh}$ drops, and the turbulent drive for the low-frequency zonal flow (the Reynolds power $\mathcal{P}_{Re}$) collapses. Correspondingly, the turbulent particle transport increases drastically with increasing collision rates. The geodesic acoustic modes (GAMs) gain more energy from the ambient turbulence at higher densities, but have smaller shearing rate than low-frequency zonal flows. The increased density also introduces decreased adiabaticity which not only enhances the particle transport but is also related to a reduction in the eddy-tilting and the Reynolds power. Both effects may lead to the cooling of edge plasmas and therefore the onset of MHD instabilities that limit the plasma density

SARS-CoV-2 and interferon blockade

© 2020, The Author(s). The response to viral infection generally includes an activation of the adaptive immune response to produce cytotoxic T cells and neutralizing antibodies. We propose that SARS-CoV-2 activates the innate immune system through the renin-angiotensin and kallikrein-bradykinin pathways, blocks interferon production and reduces an effective adaptive immune response. This model has therapeutic implications

Renminbi Internationalisation: Precedents and Implications

While it is commonly assumed that there are no known precedents against which to benchmark the internationalisation of the Renminbi (RMB), this paper argues that the PRCs own development experience provides a useful perspective on the internationalisation debate. In particular it indicates that lessons can be learnt from both the successes and the shortcomings of efforts to internationalise the RMB in the 1970s. During this period state-owned banks in Hong Kong played a central role in mobilising finance for foreign trade. Access to Hong Kong’s developed financial institutions allowed the PRC to maximise receipts from foreign trade as well as minimise the risks of undue swings in capital flows. The paper shows that although China no longer faces foreign exchange scarcity, economic reforms have not yet resolved vulnerabilities in China’s financial institutions and as such Hong Kong’s role in mitigating the risk of undue capital swings remains

Comparison Between Acute Toxicity And Chemical Analysis Of Natural Gas Drilling Effluent Samples Using Penaeus Monodon

The regulation of point source discharges usually based on specific chemicals which describe thresholds below which no adverse impact on the receiving water. The limitation of this technique includes the fact that it does not take into account interactions among toxicants (e.g. additivity, synergism, antagonism). Therefore, toxicity testing is recommended as integrated approach for identifying toxic pollutants to complement chemical-specific analysis by assessing the sum toxicity of all components in the mixture. The objective of this research was the assessment of Penaeus monodon (PM) as an indicator species for toxicity testing through comparisons between results from 48-h LC50 and chemical analysis of the natural gas drilling effluent. Chemical analysis of effluent samples was performed in order to determine concentrations of BTEX (benzene, toluene, ethyl benzene, xylene) and heavy metals (Cr, Ni, Cu, As, Pb, Cd). Average 48-h LC50\u27s for PM was 2,94 % for effluent and 0.68 g/l for KCl. Comparison between acute toxicity and chemical analysis showed a significant correlation between toluene concentrations and 48-h LC50 in PM (p<0.05). No significant correlation was found between the detectable heavy metal concentrations and acute toxicity data in PM. Results suggest that the lack of correlation between toxicity and almost all chemical parameters indicates that toxicity testing is much more useful for monitoring toxicity of effluents than chemical analysis. Additional studies are needed to identify alternative toxicity endpoints as sensitive indicators of impacts on the environment